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Les protéines du SRAS-CoV-2 régulent les réponses inflammatoires, thrombotiques

Les protéines du SRAS-CoV-2 régulent les réponses inflammatoires, thrombotiques et diabétiques dans les fibroblastes artériels humains 

Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is accountable for many pathological processes, together with altered vascular illness growth, dysfunctional thrombosis and a heightened irritation. Nonetheless, there’s restricted work to find out the underlying mobile responses induced by publicity to SARS-CoV-2 proteins. Thus, our goal was to analyze how human arterial adventitial fibroblasts irritation, thrombosis and diabetic illness markers are altered in response to Spike, Nucleocapsid and Membrane-Envelope Proteins.

We hypothesized that after a short-term publicity to SARS-CoV-2 proteins, adventitial fibroblasts would have the next expression of inflammatory, thrombotic and diabetic proteins, which might assist a mechanism for altered vascular illness development. After incubation, the expression of gC1qR, ICAM-1, tissue issue, RAGE and GLUT-Four was considerably up-regulated. Generally, the extent of expression was completely different for every SARS-CoV-2 proteins, suggesting that SARS-CoV-2 proteins interacts with cells by completely different mechanisms. Thus, SARS-CoV-2 protein interplay with vascular cells might regulate vascular illness responses.

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Immortalized Human Pulmonary Fibroblasts Cells

T0490 1x106 cells / 1.0 ml Ask for price

Retinal Pigment Epithelial Cells

RPE001 500,000 Cells
EUR 1288

Immortalized Human Mammary Epithelial Cells

T0450 1x106 cells / 1.0 ml
EUR 1036

Immortalized Human Lung Epithelial Cells

T0488 1x106 cells / 1.0 ml Ask for price

Immortalized Human Tracheal Epithelial Cells

T0489 1x106 cells / 1.0 ml Ask for price

Immortalized Human Corneal Epithelial Cells

T0572 1x106 cells / 1.0 ml
EUR 746

Immortalized Human Prostate Epithelial Cells

T0600 1x106 cells / 1.0 ml
EUR 630

Immortalized Human Renal Epithelial Cells

T0616 1x106 cells / 1.0 ml Ask for price

Immortalized Human Urethral Epithelial Cells

T0629 1x106 cells / 1.0 ml Ask for price

Epithelial Dissociation System 11 (Epithelial Cells), Mouse and Rat

4-20261 ea Ask for price

Mouse Thyroid PrimaCell: Thyroid Epithelial Cells

2-82103 1 Kit Ask for price

Rat Thyroid PrimaCell: Thyroid Epithelial Cells

2-82598 1 Kit Ask for price

Human Thyroid PrimaCell: Thyroid Epithelial Cells

2-96124 1 Kit Ask for price

Human Renal Proximal Tubule Epithelial Cells

SC00A1-PTEC 500,000 Cells
EUR 1321

Immortalized Human Pterygium Epithelial Cells - SV40

T0360 1x106 cells / 1.0 ml Ask for price

Immortalized Human Conjuctival Epithelial Cells - SV40

T0361 1x106 cells / 1.0 ml Ask for price

Immortalized Human Nasal Epithelial Cells - SV40

T0440 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Mammary Epithelial Cells (iMMEC)

T0451 1x106 cells / 1.0 ml Ask for price

Immortalized Human Bronchial Epithelial Cells - SV40

T0486 1x106 cells / 1.0 ml
EUR 1036

Immortalized Human Bronchial Epithelial Cells-hTERT

T0496 1x106 cells / 1.0 ml
EUR 1036

Immortalized Human Placental Epithelial Cells - SV40

T0530 1x106 cells / 1.0 ml Ask for price

Immortalized Human Amniotic Epithelial Cells - SV40

T0531 1x106 cells / 1.0 ml Ask for price

Immortalized Human Retinal Pigment Epithelial Cells

T0571 1x106 cells / 1.0 ml
EUR 630

Immortalized Human Lens Epithelial Cells - SV40

T0573 1x106 cells / 1.0 ml
EUR 746

Immortalized Mouse Prostate Epithelial Cells (iMPEC)

T0607 1x106 cells / 1.0 ml Ask for price

Immortalized Ovarian Surface Epithelial Cells (iMOSEC)

T0608 1x106 cells / 1.0 ml Ask for price

Immortalized Human Renal Cortical Epithelial Cells

T0614 1x106 cells / 1.0 ml Ask for price

Immortalized Human Renal Medullary Epithelial Cells

T0615 1x106 cells / 1.0 ml Ask for price

Immortalized Human Nasopharyngeal Epithelial Cells (NP69SV40T)

T0634 1x106 cells / 1.0 ml Ask for price

Immortalized Human Bronchial Epithelial Cells (HBE1)

T0680 1x106 cells / 1.0 ml
EUR 572

Immortalized Human Ovarian Epithelial Cells - SV40

T1074 1x106 cells / 1.0 ml Ask for price

Goat Anti-Camel IgG-RPE conjugate

30835-RPE 0.5 ml
EUR 263

Pan B-Cells (Ig Light Chain) Antibody (RPE)

abx413492-100tests 100 tests
EUR 718
  • Shipped within 1 week.

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-82006 1 Kit Ask for price

Mouse Cervix PrimaCell1: Normal Cervical Epithelial Cells

2-82023 1 Kit Ask for price

Mouse Cervix PrimaCell4: Normal Endometrial Epithelial Cells

2-82026 1 Kit Ask for price

Mouse Colon PrimaCell: Normal Colorectal Epithelial Cells

2-82028 1 Kit Ask for price

Mouse Esophagus PrimaCell1: Normal Esophageal Epithelial Cells

2-82035 1 Kit Ask for price

Mouse Eye PrimaCell2: Normal Corneal Epithelial Cells

2-82038 1 Kit Ask for price

Mouse Eye PrimaCell4: Normal Lens Epithelial Cells

2-82040 1 Kit Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82057 1 Kit Ask for price

Mouse Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-82077 1 Kit Ask for price

Mouse Prostate PrimaCell: Normal Prostate Epithelial Cells

2-82083 1 Kit Ask for price

Mouse Ureter PrimaCell: Normal Ureter Epithelial Cells

2-82092 1 Kit Ask for price

Mouse Urethral PrimaCell: Normal Urethral Epithelial Cells

2-82093 1 Kit Ask for price

Mouse Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82102 1 Kit Ask for price

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-82506 1 Kit Ask for price

Rat Cervix PrimaCell1: Normal Cervical Epithelial Cells

2-82524 1 Kit Ask for price

Rat Cervix PrimaCell4: Normal Endometrial Epithelial Cells

2-82527 1 Kit Ask for price

Rat Colon PrimaCell1: Normal Colorectal Epithelial Cells

2-82529 1 Kit Ask for price

Rat Esophagus PrimaCell1: Normal Esophageal Epithelial Cells

2-82536 1 Kit Ask for price

Rat Eye PrimaCell2: Normal Corneal Epithelial Cells

2-82539 1 Kit Ask for price

Rat Eye PrimaCell4: Normal Lens Epithelial Cells

2-82541 1 Kit Ask for price

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82556 1 Kit Ask for price

Rat Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-82576 1 Kit Ask for price

Rat Prostate PrimaCell: Normal Prostate Epithelial Cells

2-82580 1 Kit Ask for price

Rat Ureter PrimaCell: Normal Ureter Epithelial Cells

2-82588 1 Kit Ask for price

Rat Urethral PrimaCell: Normal Urethral Epithelial Cells

2-82589 1 Kit Ask for price

Rat Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82597 1 Kit Ask for price

Human Airway PrimaCell1: Normal Airway Epithelial Cells

2-96001 1 Kit Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-96007 1 Kit Ask for price

Human Cervix PrimaCell1: Normal Cervical Epithelial Cells

2-96044 1 Kit Ask for price

Human Cervix PrimaCell4: Normal Endometrial Epithelial Cells

2-96047 1 Kit Ask for price

Human Esophagus PrimaCell1: Normal Esophageal Epithelial Cells

2-96055 1 Kit Ask for price

Human Eye PrimaCell2: Normal Corneal Epithelial Cells

2-96058 1 Kit Ask for price

Human Eye PrimaCell4: Normal Lens Epithelial Cells

2-96060 1 Kit Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-96075 1 Kit Ask for price

Human Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-96094 1 Kit Ask for price

Human Prostate PrimaCell: Normal Prostate Epithelial Cells

2-96099 1 Kit Ask for price

Human Ureter PrimaCell2: Normal Ureter Epithelial Cells

2-96112 1 Kit Ask for price

Human Urethral PrimaCell: Normal Urethral Epithelial Cells

2-96113 1 Kit Ask for price

Human Colon PrimaCell: Normal Colorectal Epithelial Cells

2-96115 1 Kit Ask for price

Human Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-96123 1 Kit Ask for price

Immortalized Baby Mouse Kidney Epithelial Cells (iBMK)

T0082 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Pancreas Epithelial Cells-Conditionally (IMPE)

T0156 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A) - SV40

T0368 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) - SV40

T0369 1x106 cells / 1.0 ml Ask for price

Immortalized Human Mammary MUC+ESA+ Epithelial Cells

T0453 1x106 cells / 1.0 ml Ask for price

Immortalized Human Mammary Epithelial Cells (HMEC 2.6)

T0454 1x106 cells / 1.0 ml
EUR 1036

Immortalized Human Small Airway Epithelial Cells - SV40

T0485 1x106 cells / 1.0 ml
EUR 1036

Immortalized Mouse Lung Epithelial Cells (MLE-15)

T0495 1x106 cells / 1.0 ml
EUR 572

Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4

T0497 1x106 cells / 1.0 ml
EUR 1036

Immortalized Human Bronchial Epithelial Cells-hTERT/BMI

T0498 1x106 cells / 1.0 ml
EUR 1036

Immortalized Mouse Colonic Epithelial Cells-Conditionally (YAMC)

T0567 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Stomach Epithelial Cells-Conditionally (ImSt)

T0569 1x106 cells / 1.0 ml Ask for price

Immortalized Human Esophageal Epithelial Cells (NE2-hTERT)

T0632 1x106 cells / 1.0 ml
EUR 1036

Immortalized Human Nasopharyngeal Epithelial Cells (NPEC1/Bmi1)

T0765 1x106 cells / 1.0 ml Ask for price

Immortalized Human Nasopharyngeal Epithelial Cells (NPEC2/Bmi1)

T0766 1x106 cells / 1.0 ml Ask for price

Cartilage Dissociation System 1 (Mesenchymal, Fibroblasts, Epithelial-like), Mouse and Rat

4-20231 ea Ask for price

Goat Anti-Llama IgG (H+L chain)-RPE conjugate

30817-RPE 0.5 ml
EUR 263

Native Human Fibroblasts

SC00A5 500,000 Cells
EUR 905

Mouse Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82021 1 Kit Ask for price

Mouse Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells

2-82041 1 Kit Ask for price

Mouse Intestine PrimaCell3: Normal Intestinal Mucosa Epithelial Cells

2-82053 1 Kit Ask for price

Mouse Kidney PrimaCell8: Normal Renal Tubular Epithelial Cells

2-82063 1 Kit Ask for price

Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-82088 1 Kit Ask for price

Mouse Lung PrimaCell 7: Alveolar Epithelial Cells II

2-82101 1 Kit Ask for price

Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82522 1 Kit Ask for price

Rat Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells

2-82542 1 Kit Ask for price

Rat Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells

2-82552 1 Kit Ask for price

Rat Kidney PrimaCell8: Normal Renal Tubular Epithelial Cells

2-82562 1 Kit Ask for price

Rat Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-82585 1 Kit Ask for price

Rat Lung PrimaCell 7: Alveolar Epithelial Cells II

2-82596 1 Kit Ask for price

Human Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-96023 1 Kit Ask for price

Human Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells

2-96061 1 Kit Ask for price

Human Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells

2-96071 1 Kit Ask for price

Human Kidney PrimaCell7: Normal Renal Tubular Epithelial Cells

2-96080 1 Kit Ask for price

Human Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-96104 1 Kit Ask for price

Human Lung PrimaCell 7: Alveolar Epithelial Cells II

2-96122 1 Kit Ask for price

Immortalized Human Mammary Epithelial Cells (HMEC 2.6) - SV40

T0455 1x106 cells / 1.0 ml
EUR 1036

Immortalized Mouse Small Intestinal Epithelial Cells-Conditionally (MSIE)

T0568 1x106 cells / 1.0 ml Ask for price

Immortalized Human Tonsillar Epithelial Cells (E6/E7 HTE)

T0660 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Prolactin-Responsive Mammary Epithelial Cells (HC11)

T0672 1x106 cells / 1.0 ml Ask for price

Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1)

T0681 1x106 cells / 1.0 ml
EUR 572

Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) Antibody (RPE)

abx414935-100tests 100 tests
EUR 690
  • Shipped within 1 week.

Mouse Thyroid PrimaCell: Normal Thyroid Epithelial Cells Growth Medium

9-32103 5 x 100 ml Ask for price

Human Airway PrimaCell1: Normal Airway Epithelial Cells Growth Medium

9-46001 5 x 100 ml Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-46007 5 x 100 ml Ask for price

Human Cervix PrimaCell1: Normal Cervical Epithelial Cells Growth Medium

9-46044 5 x 100 ml Ask for price

Human Cervix PrimaCell4: Normal Endometrial Epithelial Cells Growth Medium

9-46047 5 x 100 ml Ask for price

Human Esophagus PrimaCell1: Normal Esophageal Epithelial Cells Growth Medium

9-46055 5 x 100 ml Ask for price

Human Eye PrimaCell2: Normal Corneal Epithelial Cells Growth Medium

9-46058 5 x 100 ml Ask for price

Human Eye PrimaCell4: Normal Lens Epithelial Cells Growth Medium

9-46060 5 x 100 ml Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-46075 5 x 100 ml Ask for price

Human Ovary PrimaCell1: Normal Ovarian Epithelial Cells Growth Medium

9-46094 5 x 100 ml Ask for price

Human Prostate PrimaCell: Normal Prostate Epithelial Cells Growth Medium

9-46099 5 x 100 ml Ask for price

Human Ureter PrimaCell2: Normal Ureter Epithelial Cells Growth Medium

9-46112 5 x 100 ml Ask for price

Human Urethral PrimaCell: Normal Urethral Epithelial Cells Growth Medium

9-46113 5 x 100 ml Ask for price

Human Colon PrimaCell: Normal Colorectal Epithelial Cells Growth Medium

9-46115 5 x 100 ml Ask for price

Human Thyroid PrimaCell: Normal Thyroid Epithelial Cells Growth Medium

9-46124 5 x 100 ml Ask for price

Human Prostate Tissue Preparation Buffer: Normal Prostate Epithelial Cells

9-80099 1 x 100 ml Ask for price

Human Urethral Tissue Preparation Buffer: Normal Urethral Epithelial Cells

9-80113 1 x 100 ml Ask for price

Mouse Colon Tissue Preparation Buffer: Normal Colorectal Epithelial Cells

9-80142 1 x 100 ml Ask for price

Mouse Prostate Tissue Preparation Buffer: Normal Prostate Epithelial Cells

9-80197 1 x 100 ml Ask for price

Mouse Ureter Tissue Preparation Buffer: Normal Ureter Epithelial Cells

9-80206 1 x 100 ml Ask for price

Mouse Urethral Tissue Preparation Buffer: Normal Urethral Epithelial Cells

9-80207 1 x 100 ml Ask for price

Rat Prostate Tissue Preparation Buffer: Normal Prostate Epithelial Cells

9-80287 1 x 100 ml Ask for price

Rat Ureter Tissue Preparation Buffer: Normal Ureter Epithelial Cells

9-80295 1 x 100 ml Ask for price

Rat Urethral Tissue Preparation Buffer: Normal Urethral Epithelial Cells

9-80296 1 x 100 ml Ask for price

Human Colon Tissue Preparation Buffer: Normal Colorectal Epithelial Cells

9-80297 1 x 100 ml Ask for price

Mouse Thyroid Tissue Preparation Buffer: Normal Thyroid Epithelial Cells

9-80309 1 x 100 ml Ask for price

Human Thyroid Tissue Preparation Buffer: Normal Thyroid Epithelial Cells

9-80317 1 x 100 ml Ask for price

Rat Thyroid Tissue Preparation Buffer: Normal Thyroid Epithelial Cells

9-80325 1 x 100 ml Ask for price

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-25006 5 x 100 ml Ask for price

Rat Cervix PrimaCell1: Normal Cervical Epithelial Cells Growth Medium

9-25024 5 x 100 ml Ask for price

Rat Cervix PrimaCell4: Normal Endometrial Epithelial Cells Growth Medium

9-25027 5 x 100 ml Ask for price

Rat Colon PrimaCell1: Normal Colorectal Epithelial Cells Growth Medium

9-25029 5 x 100 ml Ask for price

Rat Esophagus PrimaCell1: Normal Esophageal Epithelial Cells Growth Medium

9-25036 5 x 100 ml Ask for price

Rat Eye PrimaCell2: Normal Corneal Epithelial Cells Growth Medium

9-25039 5 x 100 ml Ask for price

Rat Eye PrimaCell4: Normal Lens Epithelial Cells Growth Medium

9-25041 5 x 100 ml Ask for price

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-25056 5 x 100 ml Ask for price

Rat Ovary PrimaCell1: Normal Ovarian Epithelial Cells Growth Medium

9-25076 5 x 100 ml Ask for price

Rat Prostate PrimaCell: Normal Prostate Epithelial Cells Growth Medium

9-25080 5 x 100 ml Ask for price

Rat Ureter PrimaCell: Normal Ureter Epithelial Cells Growth Medium

9-25088 5 x 100 ml Ask for price

Rat Urethral PrimaCell: Normal Urethral Epithelial Cells Growth Medium

9-25089 5 x 100 ml Ask for price

Rat Thyroid PrimaCell: Normal Thyroid Epithelial Cells Growth Medium

9-25098 5 x 100 ml Ask for price

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-32006 5 x 100 ml Ask for price

Mouse Cervix PrimaCell1: Normal Cervical Epithelial Cells Growth Medium

9-32023 5 x 100 ml Ask for price

Mouse Cervix PrimaCell4: Normal Endometrial Epithelial Cells Growth Medium

9-32026 5 x 100 ml Ask for price

Mouse Colon PrimaCell: Normal Colorectal Epithelial Cells Growth Medium

9-32028 5 x 100 ml Ask for price

Mouse Esophagus PrimaCell1: Normal Esophageal Epithelial Cells Growth Medium

9-32035 5 x 100 ml Ask for price

Mouse Eye PrimaCell2: Normal Corneal Epithelial Cells Growth Medium

9-32038 5 x 100 ml Ask for price

Mouse Eye PrimaCell4: Normal Lens Epithelial Cells Growth Medium

9-32040 5 x 100 ml Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-32057 5 x 100 ml Ask for price

Mouse Ovary PrimaCell1: Normal Ovarian Epithelial Cells Growth Medium

9-32077 5 x 100 ml Ask for price

Mouse Prostate PrimaCell: Normal Prostate Epithelial Cells Growth Medium

9-32083 5 x 100 ml Ask for price

Mouse Ureter PrimaCell: Normal Ureter Epithelial Cells Growth Medium

9-32092 5 x 100 ml Ask for price

Mouse Urethral PrimaCell: Normal Urethral Epithelial Cells Growth Medium

9-32093 5 x 100 ml Ask for price

Mouse Airway PrimaCell1: Normal Bronchial and Tracheal Epithelial Cells

2-82001 1 Kit Ask for price

Rat Airway PrimaCell1: Normal Bronchial and Tracheal Epithelial Cells

2-82501 1 Kit Ask for price

Human Airway PrimaCell2: Normal Bronchial and Tracheal Epithelial Cells

2-96002 1 Kit Ask for price

Immortalized Human Mammary Epithelial Progenitor (K5+/K19+) Cells - hTERT

T0452 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Retinal Pigmented Epithelial Cells - HPV16 E6/E7

T0574 1x106 cells / 1.0 ml Ask for price

Immortalized Human Endocervical Epithelial Cells- HPV E6/E7 (A2EN)

T0595 1x106 cells / 1.0 ml
EUR 688

Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) Antibody (RPE)

abx414364-100tests 100 tests
EUR 592
  • Shipped within 1 week.

Nuclear Factor of Activated T-Cells, Cytoplasmic 1 (NFATC1) Antibody (RPE)

abx445938-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) Antibody (RPE)

abx414846-25tests 25 tests
EUR 314
  • Shipped within 1 week.

Human Fibroblasts-GFP Expressing

GFP07 500,000 Cells in T25 flask
EUR 1354

FBS, Fibroblasts Optimized I

F0691-050 500ml Ask for price

TIMP-2, human fibroblasts

7788-5
EUR 338

Immortalized Human Cardiac Fibroblasts

T0446 1x106 cells / 1.0 ml Ask for price

Immortalized Human Uterine Fibroblasts

T0610 1x106 cells / 1.0 ml Ask for price

Dendritic Cells/B Cells Antibody

abx412775-02mg 0.2 mg
EUR 565
  • Shipped within 1 week.

RPE antibody

70R-4057 50 ug
EUR 467
Description: Rabbit polyclonal RPE antibody raised against the N terminal of RPE

RPE antibody

70R-4336 50 ug
EUR 467
Description: Rabbit polyclonal RPE antibody raised against the middle region of RPE

RPE antibody

70R-19953 50 ul
EUR 435
Description: Rabbit polyclonal RPE antibody

RPE Antibody

1-CSB-PA853385LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against RPE. Recognizes RPE from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

RPE Antibody

1-CSB-PA020097GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity Purified
Description: A polyclonal antibody against RPE. Recognizes RPE from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

Mouse Pancreas PrimaCell 2: Normal Pancreatic Epithelial Cells Growth Medium

9-32102 5 x 100 ml Ask for price

Human Breast PrimaCell: Normal Mammary Epithelial Primary Cells Growth Medium

9-46023 5 x 100 ml Ask for price

La myostatine régule la manufacturing du facteur de croissance 23 des fibroblastes (FGF23) dans les cellules de kind ostéoblaste UMR106 

Myostatin is a signaling molecule produced by skeletal muscle cells (myokine) that inhibits muscle hypertrophy and has additional paracrine and endocrine results in different organs together with bone. Myostatin binds to activin receptor kind 2B which varieties a posh with remodeling development factor-β kind I receptor (TGF-βRI) and induces intracellular p38MAPK and NFκB signaling. Fibroblast development issue 23 (FGF23) is a paracrine and endocrine mediator produced by bone cells and regulates phosphate and vitamin D metabolism within the kidney. P38MAPK and NFκB-dependent store-operated Ca2+ entry (SOCE) are optimistic regulators of FGF23 manufacturing.

Right here, we explored whether or not myostatin influences the synthesis of FGF23. Fgf23 gene expression was decided by qRT-PCR and FGF23 protein by ELISA in UMR106 osteoblast-like cells. UMR106 cells expressed activin receptor kind 2A and B. Myostatin upregulated Fgf23 gene expression and protein manufacturing. The myostatin impact on Fgf23 was considerably attenuated by TGF-βRI inhibitor SB431542, p38MAPK inhibitor SB202190, and NFκB inhibitor withaferin A. Furthermore, SOCE inhibitor 2-APB blunted the myostatin impact on Fgf23. Taken collectively, myostatin is a stimulator of Fgf23 expression in UMR106 cells, an impact not less than partially mediated by downstream TGF-βRI/p38MAPK signaling in addition to NFκB-dependent SOCE.

La diaphonie desmoplasique dans l’adénocarcinome canalaire pancréatique est reflétée par différentes réponses des lignées cellulaires Panc-1, MIAPaCa-2, PaTu-8902 et CAPAN-2 aux fibroblastes normaux associés au most cancers 

Background/intention: Pancreatic ductal adenocarcinoma (PDAC) nonetheless represents one of the vital aggressive cancers. Understanding of the epithelial-mesenchymal crosstalk as a vital a part of the tumor microenvironment ought to pave the best way for therapies to enhance affected person survival charges. Nicely-established cell strains current a helpful and reproducible mannequin to review PDAC biology. Nonetheless, the tumor-stromal interactions between most cancers cells and cancer-associated fibroblasts (CAFs) are nonetheless poorly understood.

Supplies and strategies: We studied interactions between 4 PDAC cell strains (Panc-1, CAPAN-2, MIAPaCa-2, and PaTu-8902) and conditioned media derived from major cultures of regular fibroblasts/PDAC-derived CAFs (PANFs).

Outcomes: When the examined PDAC cell strains have been stimulated by PANF-derived conditioned media, probably the most aggressive habits was acquired by the Panc-1 cell line (elevated quantity and dimension of colonies, remaining expression of vimentin and keratin eight in addition to improve of epithelial-to-mesenchymal polarization markers), whereas PaTu-8902 cells have been somewhat inhibited. Of be aware, administration of the conditioned media to MIAPaCa-2 cells resulted in an inverse impact on the dimensions and variety of colonies, whereas CAPAN-2 cells have been somewhat stimulated.

To clarify the heterogeneous sample of the noticed PDAC crosstalk on the in vitro stage, we additional in contrast the phenotype of major cultures of cells derived from ascitic fluid with that of the examined PDAC cell strains, analyzed tumor samples of PDAC sufferers, and carried out gene expression profiling of PANFs. Immuno-cyto/histo-chemical evaluation discovered particular phenotype variations inside the group of examined sufferers and examined PDAC cell strains, whereas the genomic strategy in PANFs discovered the important thing molecules (IL6, IL8, MFGE8 and periostin) which will contribute to the most cancers aggressive habits.

Conclusion: The desmoplastic patient-specific regulation of most cancers cells by CAFs (additionally demonstrated by the heterogeneous response of PDAC cell strains to fibroblasts) precludes easy focusing on and growth of an efficient remedy technique and somewhat requires institution of an individualized tumor-specific remedy protocol.

 

Analyse protéomique quantitative sans étiquette des vésicules extracellulaires libérées par des fibroblastes provenant de sufferers atteints d’amyotrophie spinale

 

Spinal muscular atrophy (SMA) is an autosomal recessive dysfunction that represents a big reason for toddler mortality. SMA is characterised by decreased ranges of the Survival Motor Neuron protein resulting in the lack of alpha motor neurons within the spinal wire and mind stem in addition to defects in peripheral tissues equivalent to skeletal muscle and liver.

With progress in promising therapies equivalent to antisense oligonucleotide and gene alternative, there stays a necessity to raised perceive illness subtypes and develop biomarkers for improved diagnostics and therapeutic monitoring. On this examine, we’ve got examined the utility of extracellular vesicles as a supply of biomarker discovery in patient-derived fibroblast cells. Proteome examination using data-independent acquisition and ion mobility mass spectrometry recognized 684 protein teams current in all organic replicates examined.

Label-free quantitative evaluation recognized 116 statistically important protein alterations in comparison with management cells, together with a number of identified SMA biomarkers. Protein stage variations have been additionally noticed in regulators of Wnt signaling and Cajal our bodies. Lastly, ranges of insulin development issue binding protein-Three was validated as being considerably greater in extracellular vesicles remoted from SMA cells.

We conclude that extracellular vesicles signify a promising supply for SMA biomarker discovery in addition to a related constituent for advancing our understanding of SMA pathophysiology. This text is protected by copyright. All rights reserved.

 

Sos1 module la synthèse, la prolifération et la migration de la matrice extracellulaire dans les fibroblastes 

Non-reversible fibrosis is frequent in varied illnesses equivalent to persistent renal failure, liver cirrhosis, persistent pancreatitis, pulmonary fibrosis, rheumatoid arthritis and atherosclerosis. Reworking development issue beta 1 (TGF-β1) is concerned in nearly all varieties of fibrosis. We beforehand described the involvement of Ras GTPase isoforms within the regulation of TGF-β1-induced fibrosis.

The guanine nucleotide trade issue Son of Sevenless (Sos) is the principle Ras activator, however the position of the ubiquitously expressed Sos1 within the growth of fibrosis has not been studied. For this function, we remoted and cultured Sos1 knock-out (KO) mouse embryonic fibroblasts, the principle extracellular matrix proteins (ECM)-producing cells, and we analyzed ECM synthesis, cell proliferation and migration within the absence of Sos1, in addition to the position of the principle Sos1-Ras effectors, Erk1/2 and Akt, in these processes.

The absence of Sos1 will increase collagen I expression (by the PI3K-Akt signaling pathway), complete collagen proteins, and barely will increase fibronectin expression; Sos1 regulates fibroblast proliferation by each PI3K-Akt and Raf-Erk pathways, and Sos1-PI3K-Akt signaling regulates fibroblast migration.

These examine exhibits that Sos1 regulates ECM synthesis and migration (by Ras-PI3K-Akt) and proliferation (by Ras-PI3K-Akt and Ras-Raf-Erk) in fibroblasts, and describe for the primary time the position of the Sos1-Ras signaling axis within the regulation of mobile processes concerned within the growth of fibrosis.

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