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Les protéines du SRAS-CoV-2 régulent les réponses inflammatoires, thrombotiques

Les protéines du SRAS-CoV-2 régulent les réponses inflammatoires, thrombotiques et diabétiques dans les fibroblastes artériels humains 

Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is accountable for many pathological processes, together with altered vascular illness growth, dysfunctional thrombosis and a heightened irritation. Nonetheless, there’s restricted work to find out the underlying mobile responses induced by publicity to SARS-CoV-2 proteins. Thus, our goal was to analyze how human arterial adventitial fibroblasts irritation, thrombosis and diabetic illness markers are altered in response to Spike, Nucleocapsid and Membrane-Envelope Proteins.

We hypothesized that after a short-term publicity to SARS-CoV-2 proteins, adventitial fibroblasts would have the next expression of inflammatory, thrombotic and diabetic proteins, which might assist a mechanism for altered vascular illness development. After incubation, the expression of gC1qR, ICAM-1, tissue issue, RAGE and GLUT-Four was considerably up-regulated. Generally, the extent of expression was completely different for every SARS-CoV-2 proteins, suggesting that SARS-CoV-2 proteins interacts with cells by completely different mechanisms. Thus, SARS-CoV-2 protein interplay with vascular cells might regulate vascular illness responses.

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Mouse Anti Human Fibroblasts/Epithelial Cells Monoclonal Antibody,RPE

DMABT-49595MH 25 TEST
EUR 514.8

Fibroblasts, Epithelial Cells

MBS609129-02mg 0.2mg
EUR 820

Fibroblasts, Epithelial Cells

MBS609129-5x02mg 5x0.2mg
EUR 3550

Fibroblasts, Epithelial Cells (AP)

MBS6247938-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (AP)

MBS6247938-5x01mL 5x0.1mL
EUR 4815

OASA02809-25UG - FIBROBLASTS/EPITHELIAL CELLS Antibody

OASA02809-25UG 25ug
EUR 149

OASA02808-200UG - FIBROBLASTS/EPITHELIAL CELLS Antibody

OASA02808-200UG 0.2mg
EUR 499

Fibroblasts, Epithelial Cells (HRP)

MBS6250880-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (HRP)

MBS6250880-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (APC)

MBS6268791-01mL 0.1mL
EUR 1105

Fibroblasts, Epithelial Cells (APC)

MBS6268791-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (FITC)

MBS6249884-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (FITC)

MBS6249884-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (Biotin)

MBS6248958-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (Biotin)

MBS6248958-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (MaxLight 490)

MBS6253886-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (MaxLight 490)

MBS6253886-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (MaxLight 550)

MBS6254926-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (MaxLight 550)

MBS6254926-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (MaxLight 405)

MBS6252846-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (MaxLight 405)

MBS6252846-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (MaxLight 750)

MBS6257005-01mL 0.1mL
EUR 1105

Fibroblasts, Epithelial Cells (MaxLight 750)

MBS6257005-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (MaxLight 650)

MBS6255966-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (MaxLight 650)

MBS6255966-5x01mL 5x0.1mL
EUR 4815

MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS

MBS214991-02mg 0.2mg
EUR 550

MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS

MBS214991-5x02mg 5x0.2mg
EUR 2295

MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS

MBS214992-0025mg 0.025mg
EUR 225

MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS

MBS214992-5x0025mg 5x0.025mg
EUR 835

OASA02810-100T - Fibroblasts/Epithelial Cells Antibody - PE Conjugated

OASA02810-100T 100Tests
EUR 519

OASA02811-25T - Fibroblasts/Epithelial Cells Antibody - PE Conjugated

OASA02811-25T 25Tests
EUR 219

Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, PE

SM1214R 100 Tests Ask for price

Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, PE

SM1214RT 25 Tests Ask for price

MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE

MBS214993-100Tests 100Tests
EUR 575

MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE

MBS214993-5x100Tests 5x100Tests
EUR 2410

MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE

MBS214994-25Tests 25Tests
EUR 270

MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE

MBS214994-5x25Tests 5x25Tests
EUR 1035

Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, Purified

SM1214P 200 µg Ask for price

Cartilage Dissociation System 1 (Mesenchymal, Fibroblasts, Epithelial-like), Mouse and Rat

4-20231 ea Ask for price

Human Skin Cells (Fibroblasts)

ABC-TC3807 1 vial Ask for price
Description: Human fibroblasts are derived from cultured skin explants. These fibroblasts are from a single donor.

H-RPE (Human Retinal Pigment Epithelial Cells)

LO194987 each
EUR 1380.98

Human Primary Pulmonary Fibrosis Bronchial Epithelial Cells

T5462 1 x 10^6 / 1.0 ml Ask for price

Human Skin Cells (Fibroblasts, Prenata)

ABC-TC3809 1 vial Ask for price
Description: Human fibroblasts are derived from cultured skin explants. Human fibroblasts are from a single donor. These cells enable researchers to study skin diseases such as dermatitis, wound healing, and other diseases that are expressed in fibroblast. In addition, they may be used to study the development of skin and production of inducible pluripotent stem cells. Development period: Prenata

Human Skin Cells (Fibroblasts, Postnatal)

ABC-TC3808 1 vial Ask for price
Description: Human fibroblasts are derived from cultured skin explants. Human fibroblasts are from a single donor. These cells enable researchers to study skin diseases such as dermatitis, wound healing, and other diseases that are expressed in fibroblast. In addition, they may be used to study the development of skin and production of inducible pluripotent stem cells. Development period: Postnatal

Human Primary ∆F508/∆1152H Cystic Fibrosis Bronchial Epithelial Cells

T5425 5 x 10^5 / 1.0 ml Ask for price

DHBE-CF (Diseased Human Bronchial Epithelial Cells - Cystic Fibrosis)

LO196979 each
EUR 2914.41

Human Primary DF508/DF508 Cystic Fibrosis Bronchial Epithelial Cells

T5423 5 x 10^5 / 1.0 ml Ask for price

Human Primary DF508/N1303K Cystic Fibrosis Bronchial Epithelial Cells

T5424 5 x 10^5 / 1.0 ml Ask for price

Human Primary DF508/S549N Cystic Fibrosis Bronchial Epithelial Cells

T5427 1 x 10^6 / 1.0 ml Ask for price

D-SAEC (Diseased Small Airway Epithelial Cells – Cystic Fibrosis), cryo

LOCC-2933 each
EUR 2181.55

Immortalized Human Pulmonary Fibroblasts Cells

T0490 1x106 cells / 1.0 ml
EUR 2750

Immortalized Human Pulmonary Fibroblasts Cells

CSC-I9127L One Frozen vial Ask for price

Mouse Fibroblasts cryo amp, 4 mil cells

LOM-FB-481 each
EUR 895.66

Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1)

T0681 1x106 cells / 1.0 ml
EUR 686.4

Rat Lens Epithelial Cells

ABC-TC4150 1 vial Ask for price
Description: The cells that make up the mammalian lens consist of two types: lens fiber cells, which form the bulk of the lens, and a monolayer of epithelial cells cover the anterior surface of the fibers.

Human Primary DF508/621+1G>T Cystic Fibrosis Bronchial Epithelial Cells

T5426 5 x 10^5 / 1.0 ml Ask for price

Rat Liver Epithelial Cells

ABC-TC4152 1 vial Ask for price
Description: Rat Liver Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Liver Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Liver Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Liver Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells-SV40

CSC-I2271Z One Frozen vial Ask for price

Human Dermal Fibroblasts-fetal Feeder Cells

T2002 10^6 cells/1.0 ml
EUR 200

Human Lens Epithelial Cells

ABC-TC3669 1 vial Ask for price
Description: Human Lens Epithelial Cells from Gentaur Research Laboratories are isolated from the human lens. HLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLEpiC are guaranteed to further culture in the conditions provided by Gentaur Research Laboratories.

Rat Cornea Epithelial Cells

ABC-TC4115 1 vial Ask for price
Description: Rat cornea epithelial cells, 3-week Wistar rat

Rat Dermal Epithelial Cells

ABC-TC4118 1 vial Ask for price
Description: Rat Dermal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Dermal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Dermal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Dermal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Kidney Epithelial Cells

ABC-TC4146 1 vial Ask for price
Description: Rat Kidney Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Kidney Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Kidney Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Kidney Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Spleen Epithelial Cells

ABC-TC4221 1 vial Ask for price
Description: Rat Spleen Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Spleen Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Spleen Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Spleen Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Human Oral Epithelial Cells

ABC-TC4365 1 vial Ask for price
Description: Cells are only guaranteed with purchase of Gentaur Media, Extra Cellular Matrix, Trypsin EDTA, 1X PBS, and freezing media for the appropriate cell culture, for 30 days from the date of shipment.

Human Lens Epithelial Cells

CSC-7764W One Frozen vial Ask for price

Human Nasal Epithelial Cells

ABC-TC3719 1 vial Ask for price
Description: The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane). Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) . Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Renal Epithelial Cells

ABC-TC3782 1 vial Ask for price
Description: Human Renal Epithelial Cells(Human kidney epithelial cells) are derived from whole kidneys that have been dissociated into single cells and purified in culture. Human kidney epithelial cells are from a single donor. These are highly purified epithelial cells as determined by specific epithelial cell markers. Human kidney epithelial cells enable researchers to study the development and biochemical function of the kidneys. In addition, they are an ideal cell for studying absorption and secretion that occurs in the kidneys. Development period: Prenatal

Rat Colonic Epithelial Cells

ABC-TC4113 1 vial Ask for price
Description: Rat Primary Colonic Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Colonic Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Colonic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Colonic Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Corneal Epithelial Cells

ABC-TC4116 1 vial Ask for price
Description: Rat Corneal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Corneal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Corneal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Corneal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Mammary Epithelial Cells

ABC-TC4166 1 vial Ask for price
Description: Rat Mammary Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Mammary Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Mammary Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Mammary Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Ovarian Epithelial Cells

ABC-TC4186 1 vial Ask for price
Description: Rat Ovarian Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Ovarian Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Ovarian Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Ovarian Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Stomach Epithelial Cells

ABC-TC4228 1 vial Ask for price
Description: Rat Stomach Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Stomach Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Stomach Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Stomach Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Thyroid Epithelial Cells

ABC-TC4231 1 vial Ask for price
Description: Rat Thyroid Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Thyroid Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Thyroid Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Thyroid Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Bladder Epithelial Cells

ABC-TC5507 1 vial Ask for price
Description: Rat Primary Bladder Epithelial Cells from Gentaur are isolated from bladder tissue of 6-8 week old laboratory Sprague–Dawley rat. Rat Primary Bladder Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5x106 cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Nasal Epithelial Cells 

CSC-C1511 One Frozen vial Ask for price

Human Renal Epithelial Cells

ALHE35 1ml frozen Vial
EUR 620

Human Tonsil Epithelial Cells

ABC-TC3834 1 vial Ask for price
Description: Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of actively differentiating epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium. The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues. In Situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial culture in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ. HTonEpiC from Gentaur Research Laboratories are isolated from human normal tonsil tissue. HTonEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HTonEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HTonEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTonEpiC are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.

Porcine Lens Epithelial Cells

ABC-TC4033 1 vial Ask for price
Description: The cells that make up the mammalian lens consist of two types: lens fiber cells, which form the bulk of the lens, and a monolayer of epithelial cells that cover the anterior surface of the fibers.

Rat Prostate Epithelial Cells

ABC-TC4197 1 vial Ask for price
Description: Rat Prostate Epithelial Cells from Gentaur are isolated from tissue of adult laboratory Sprague–Dawley Rats. Rat Prostate Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Prostate Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Prostate Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Tracheal Epithelial Cells

ABC-TC4233 1 vial Ask for price
Description: Rat Primary Tracheal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Tracheal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Tracheal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Tracheal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Human Thymic Epithelial Cells

ABC-TC5517 1 vial Ask for price
Description: Thymic epithelial cells (TyEpiC) are vital regulators of thymocyte development and T lymphocyte (T-cell) tolerance. The thymus contains two types of TyEpiC, cortical and medullary thymic epithelial cells, which regulate the positive selection of thymocytes and the negative selection of autoreactive T-cells. The inability of TyEpiC to remove autoreactive T-cells in the thymus can affect self-tolerance and contribute to the development of autoimmune diseases such as myasthenia gravis, type 1 diabetes, rheumatoid arthritis, and multiple sclerosis [3]. Studies have shown that changes in the expression of tumor necrosis factor receptor family members and transcription factors Foxn1 and autoimmune regulator (Aire) can alter the thymic microenvironment and self-tolerance. Human TyEpiC are an excellent in vitro model to study the mechanisms that modulate TyEpiC functionality and to develop targeted treatments for autoimmune disorders.

Human Colonic Epithelial Cells

ABC-TC3570 1 vial Ask for price
Description: The colorectum is a major organ for both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host’s internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component of the mucosal immune system. Human colon epithelial cells can process and present antigens to T cells in vitro, and can be stimulated to express HLA class II and intercellular adhesion molecules in vivo. They also respond to a broad array of cytokines with altered gene expression and growth characteristics. In addition, normal colonic epithelial cells were found to express the integrin α3, α5, α6, β1 and β4 chains. HCoEpiC from Gentaur Research Laboratories are isolated from human colonic tissue. HCoEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCoEpiC are characterized by immunofluorescent method with antibodies to CK18 and CK19. HCoEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoEpiC are guaranteed to further culture for 15 population doublings in the conditions provided by Gentaur Research Laboratories.

Human Corneal Epithelial Cells

ABC-TC3577 1 vial Ask for price
Description: The cornea is a unique tissue for two reasons: the transparency function and the synthesis of most of its proteins due to the cornea being an avascular tissue. The cornea consists of three distinct cell layers, the outer epithelium, the inner endothelium and the central stroma (the keratocytes). The corneal epithelium plays a role in the innate immune response by sensing the presence of pathogens and providing signals that activate the corneal defense system. It also highly expresses aldehyde dehydrogenase to protect against UV- and 4-hydroxynonenal-induced cellular damage. Corneal epithelial basal cell proliferation is controlled by a host of cytokines, e.g., epidermal growth factor, that activate their cognate receptors in the deeper layers of the epithelium. Instead of testing products in the eyes of animals, cultured corneal epithelial cells could be used for toxicology tests in in vitro models and for studying the molecular mechanisms involved in regulating human corneal epithelial cell differentiation.HCEpiC from Gentaur Research Laboratories are isolated from human cornea. HCEpiC are cryopreserved from passage one culture. Each vial contains 5×10^5 cells in 1 ml volume. HCEpiC are characterized by their cobble stone morphology in serum-free culture and immunofluorescent method with antibody to CK-18 and -19. HCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCEpiC are guaranteed to further expand for 10 population doublings at the condition provided by Gentaur Research Laboratories.

Human Urothel Epithelial Cells

ABC-TC3860 1 vial Ask for price
Description: The HUEPC cultures are produced on the base of human tissues. The HUEPC are isolated according to known procedures. Then primary cultures are produced. After further culturing, the HUEPC are frozen in a computer controlled freezing unit.

Rat Bronchial Epithelial Cells

ABC-TC4284 1 vial Ask for price
Description: Rat Primary Bronchial Epithelial Cells from Gentaur are isolated from bronchial tissue of 6-8 week old laboratory Sprague–Dawley rat. Rat Primary Bronchial Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur’ Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5×10^6 cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Bladder Epithelial Cells

ABC-TC4367 1 vial Ask for price
Description: Human Primary Bladder Epithelial Cells from Gentaur are isolated from normal human bladder tissue. Human Primary Bladder Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaurandrsquo; Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Stomach Epithelial Cells

ABC-HP021X 1 vial Ask for price
Description: Human Stomach Epithelial Cells are isolated from normal human stomach tissue. Cells are cryopreserved at passage 3 and delivered frozen.

Human Corneal Epithelial Cells

cAP-0211 1Frozen Vial
EUR 594

Human Colonic Epithelial Cells

CSC-C1488 One Frozen vial Ask for price

Human Stomach Epithelial Cells

CSC-C9381W One Frozen vial Ask for price

Human Bladder Epithelial Cells

ALHE36 1ml frozen Vial
EUR 620

Human Amniotic Epithelial Cells

ABC-TC3486 1 vial Ask for price
Description: Human amniotic membrane is composed of an epithelial cell layer, a basement membrane and an avascular matrix. The amniotic epithelial cells (AEC) are formed from epiblasts on the 8th day after fertilization. A probable result of their embryonic origin, AEC lack major histocompatibility complex antigens and have been used for allotranplantation to treat patients with lysosomal diseases. Studies have shown that AEC have multiple functions such as synthesis and release of acetylcholine and catecholamine as well as expressing mRNA coding for DA receptors and DA transporters. They express neuronal and glial cell marks, produce basic fibroblast growth factors and hepatocyte growth factors and transform growth factor-beta. Human AEC has been suggested as an appropriate human cell model for studying DA release and uptake processes, receptor signal transduction and exploring newly developed drugs acting at these receptors.HAEpiC from Gentaur Research Laboratories are isolated from human amniotic membranes. HAEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HAEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Tracheal Epithelial Cells

ABC-TC3839 1 vial Ask for price
Description: The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelia Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi,and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Prostate Epithelial Cells

ABC-TC3995 1 vial Ask for price
Description: Human Prostate Epithelial Cells (HPrEC), when grown in LIPro Medium, provides an ideal serum-free culture model, without retinoic acid, for the study of prostate cells. Common uses for HPrEC are studies of hormonal regulation of the prostate, regulation and control of the secretory function of prostate cells and as a control for the study of prostate cancer. HPrEC are cryopreserved as secondary cells to ensure the highest viability and plating efficiency. Our HPrEC are quality tested in LIPro Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented media. Cell Features:HPrEC are cryopreserved as secondary cells, e.g. cells are isolated fromhuman prostate tissue and expanded twice in culture vessels before beingharvested for cryopreservation.HPrEC proliferate in a serum-free, retinoic acid-free medium withoutphenol red or antimicrobials when cultured in ProstaLifeTM medium.HPrEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Rat Esophageal Epithelial Cells

ABC-TC4133 1 vial Ask for price
Description: Rat Primary Esophageal Epithelial Cells from Gentaur are isolated from tissue of adult laboratory Sprague–Dawley Rats. Rat Primary Esophageal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Esophageal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Esophageal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Intestinal Epithelial Cells

ABC-TC4140 1 vial Ask for price
Description: Rat Intestinal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Intestinal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Intestinal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Intestinal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Pancreatic Epithelial Cells

ABC-TC4190 1 vial Ask for price
Description: Rat Pancreatic Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Pancreatic Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Pancreatic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Pancreatic Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Human Cervical Epithelial Cells

ABC-TC5466 1 vial Ask for price
Description: Cervical Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreserved to ensure the highest purity, viability and plating efficiency. HCxEC are quality tested in Epithelial Cell Medium to ensure optimal morphology and growth over a period of at least 5 population doublings. Cervical Epithelial Cells are not exposed to antimicrobials or phenol red when cultured in Epithelial Cell Medium. Gentaur offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation.

Human Alveolar Epithelial Cells

cAP-0208 1Frozen Vial
EUR 643.5

Human Alveolar Epithelial Cells

CSC-7845W One Frozen vial Ask for price

Human Prostate Epithelial Cells

ALHE25 1ml frozen Vial
EUR 620

Human Bronchial Epithelial Cells

ABC-TC3528 1 vial Ask for price
Description: The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelial Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Placental Epithelial Cells

ABC-TC3753 1 vial Ask for price
Description: The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelial Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Porcine Corneal Epithelial Cells

ABC-TC4029 1 vial Ask for price
Description: The cornea is a unique tissue for two reasons: the transparency function and the synthesis of most of its proteins due to the cornea being an avascular tissue.

Canine Alveolar Epithelial Cells

ABC-TC5511 1 vial Ask for price
Description: Canine Primary Alveolar Epithelial Cells are isolated from alveolar tissue of beagle dog. Canine Primary Alveolar Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Culture Complete Growth Medium for 3-5 days. Prior to shipping, cells at passage 2 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods.

Human Laryngeal Epithelial Cells

ABC-TC5518 1 vial Ask for price
Description: Laryngeal Epithelial Cells are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency.

Retinal Pigment Epithelial Cells

RPE001 500,000 Cells
EUR 1545.6

Human Bronchial Epithelial Cells

CSC-C4388X One Frozen vial Ask for price

Human Bronchial Epithelial Cells

ALHE47 1ml frozen Vial
EUR 620

Retinal Pigment Epithelial Cells

MBS556451-INQUIRE INQUIRE Ask for price

Human Esophageal Epithelial Cells

ABC-TC3612 1 vial Ask for price
Description: The human esophagus is lined by a non-keratinizing, moist stratified squamous epithelium whose apical cell membranes and intercellular junctional complexes combine to produce an effective permeability barrier against the influx of luminal content. In particular, the barrier created by these structures limits exposure of the surface cells' basolateral cell membranes and entire membrane of cells of the deeper layers to the wide swings in osmolality occurring regularly within the esophageal lumen. Histologically, the esophageal epithelium consists of two zones, the basal and differentiated zones. Cellular proliferation is limited to the basal zone, and cells are thought to migrate from this area towards the esophageal lumen. Migration is associated with the initiation of differentiation and the sequential expression of differentiation markers. The availability of human esophageal epithelial cell culture provides an excellent in vitro model in the study of the physiology of esophageal epithelium and the mechanisms of the esophageal carcinogenesis.HEEC from Gentaur Research Laboratories are isolated from the human esophagus. HEEC are cryopreserved on passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEEC are characterized by immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. HEEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEEC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Pancreatic Epithelial Cells

ABC-TC3744 1 vial Ask for price
Description: Human Pancreatic Epithelial Cell were initiated by elutriation of dispase dissociated normal pancreatic tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

Human Intestinal Epithelial Cells

ABC-TC3879 1 vial Ask for price
Description: Cryopreserved InEpC are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures. In combination with human intestinal myofibroblasts (InMyoFib).

Human Esophageal Epithelial Cells 

CSC-7783W One Frozen vial Ask for price

Human Pancreatic Epithelial Cells

CSC-8246W One Frozen vial Ask for price

Human Endometrial Epithelial Cells

ABC-TC4601 1 vial Ask for price
Description: Proliferation and Morphology: Normal morphology for 5 population doublings, minimum 70% viability when thawed from cryopreservation. Normal Human Endometrial (Uterine) Epithelial Cells provide an ideal serum-free culture model for many areas of research. These cells may be used to study cellular physiology of the reproductive tract, the cellular response to infectious agents, and other areas of research, Endometrial Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.

Human Conjunctival Epithelial Cells

ABC-TC3573 1 vial Ask for price
Description: The conjunctival epithelium is a stratified, non-keratinized epithelium. It can be distinguished by the corneal epithelium by the expression of different cytokeratins, mucins and by the presence of glycocalix. Decrease or loss of mucin/glycocalix production generates squamous metaplasia, which may lead to dry eye and ocular surface diseases. Human conjunctival epithelial cells represent potential target cells for mast cell mediators. They respond to histamine by generating inositol phosphates, mobilizing intracellular calcium, and then secreting proinflammatory cytokines like interleukin-6 and interleukin-8.HConEC from Gentaur Research Laboratories are isolated from human conjunctiva. HConEC are cryopreserved from passage one culture. Each vial contains 5×10^5 cells in 1 ml volume. HConEC are characterized by their cobble stone morphology in serum-free culture and immunofluorescent method with antibody to CK-18 and -19. HConEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HConEC are guaranteed to further expand for 10 population doublings at the condition provided by Gentaur Research Laboratories.

Bovine Endometrium Epithelial Cells

ABC-TC3131 1 vial Ask for price
Description: Bovine Endometrial Epithelial Cells (BEnEpC) are isolated from epithelium of the bovine endometrium. The cells are cryopreserved at second passage. BEnEpC can be cultured and propagated for at least 10 population doublings.

Human Conjunctival Epithelial Cells 

CSC-7802W One Frozen vial Ask for price

Human Nasopharyngeal Epithelial Cells

cAP-0222 1Frozen Vial
EUR 4455

Epithelial Dissociation System 11 (Epithelial Cells), Mouse and Rat

4-20261 ea Ask for price

Rat Primary Lung Epithelial Cells

T4800 5x10^5 cells / 1.0 ml
EUR 625

Primary Rabbit Retinal Pigment Epithelial Cells (RPEC)

CSI146Rb01 5X10^5
EUR 1760

Rat Renal Tubular Epithelial Cells

ABC-TC4210 1 vial Ask for price
Description: Renal tubular epithelial cells (RTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine.

Human Renal Mixed Epithelial Cells

ABC-TC4245 1 vial Ask for price
Description: Normal Human Renal Epithelial Cells, when grown in LIRen Medium, provide an ideal low-serum culture model for the study of renal function, metabolism, nephrotoxicity or cancer research. Renal Mixed Epithelial Cells are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our Renal Epithelial Cells are quality tested in LIRen Medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium. Cell Features:Mixed Renal Epithelial, Renal Cortical Epithelial, and Renal Medullary Epithelial are cryopreserved as primary cells isolated from human kidney tissue and expanded in culture vessels once before cryopreservation.Renal Proximal Tubule Epithelial are cryopreserved as secondary cells isolated from human kidney tissue and expanded in culture vessels twice before cryopreservation.All Renal Epithelial Cell types can be grown in a 0.5% serum medium without phenol red or antimicrobials when cultured in LIRen Medium.All Renal Epithelial Cell types are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Rat Primary Renal Epithelial Cells

T4936 5x10^5 cells / 1.0 ml Ask for price

Human Iris Pigment Epithelial Cells

ABC-TC3658 1 vial Ask for price
Description: The iris is a pigmented disk with a variable aperture which forms the pupil of the eye. It is covered by squamous epithelium on anterior surface and 2 layers of pigmented epithelium on posterior. The iris pigment epithelial cells (IPE) share functional properties with retinal pigment epithelial cells (RPE) such as phagocytosis, degradation of rod outer segments and synthesis of trophic factors. In recent years, IPE have been transplanted into the subretinal space of eye to treat RPE defects. The grafted IPE were seen to survive for months. There was a remodeling of cellular layers in the subretinal space over time where grafted IPE joined the native RPE. The cellular response that developed exhibited macrophages and were similar to that observed following RPE transplantation. In vitro study also show that IPE expressing CD86, directly suppresses T cell activation via binding to cytotoxic T lymphocyte-associated antigen 4.HIPEpiC from Gentaur Research Laboratories are isolated from the human iris. HIPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HIPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, vimentin and fibronectin. HIPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIPEpiC are guaranteed to further expand for two passages (around 10 population doublings) in the conditions provided by Gentaur Research Laboratories.

Human Mammary Epithelial Cells, Male

ABC-TC3693 1 vial Ask for price
Description: Human Mammary Epithelial Cells, when grown in LIMam Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.Cell Features:HMEC are cryopreserved as tertiary cells, e.g. cells are isolated from human mammary tissue and expanded three times in culture vessels before being harvested for cryopreservation.HMEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Human Small Airway Epithelial Cells

ABC-TC3811 1 vial Ask for price
Description: The small airways are located at the interface between alveoli and conducting airways. Airway epithelial cells forming a continuous lining to the airways play a unique role as a protective physical and functional barrier to external deleterious agents. These cells function in the regulation of immune responses by contributing to host defense through chemokine production, adhesion molecule expression, and possibly antigen presentation via HLA-DR expression. They also produce liquids contributing to pulmonary fluid balance. Many airway diseases, such as asthma, bronchiolitis, chronic obstructive pulmonary disease, and cystic fibrosis, involve damage to the airway surface epithelium. The human small airway epithelial cell culture may identify new therapeutic options in preventing amplified airway ailment and remodeling.HSAEpiC from Gentaur Research Laboratories are isolated from human lung tissue. HSAEpiC are cryopreserved at either primary culture or passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HSAEpiC are characterized by immunofluorescent method with antibodies CK-18, -19, and vimentin. HSAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSAEpiC are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

Human Small Airway Epithelial Cells

CSC-C9229J One Frozen vial Ask for price

Human Small Airway Epithelial Cells

ALHE50 1ml frozen Vial
EUR 620

Human Mammary Basal Epithelial Cells

ABC-TC3690 1 vial Ask for price
Description: human mammary basal epithelial cells are isolated from mammary tissue of healthy non-diabetic donors between 18 and 60 years old who have undergone elective surgery. The cells are isolated by digestion of the tissue to generate organoid structures.

Human Mammary Epithelial Cells, Adult

ABC-TC3691 1 vial Ask for price
Description: The mammary gland consists of pseudostratified epithelia, with myoepithelila cells resting upon a basement membrane and apical glandular epithelial cells facing the lumen of the ducts and alveoli. Mammary epithelial cells undergo changes in growth, invasion, and differentiation throughout much of adulthood, and most strikingly during pregnancy, lactation, and involution. They proliferate and invade the surrounding extracellular matrix during the fetal and postnatal development of the gland, and then more vigorously at puberty as the branches of the mammary epithelial tree are formed.HMEpiC from Gentaur Research Laboratories are isolated from normal human breast tissue. HMEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HMEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HMEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMEpiC are guaranteed to further expand for 15 population doublings in the conditions provided by Gentaur Research Laboratories

Human Primary Nasal Epithelial Cells

T4014 5x10^5 cells / 1.0 ml
EUR 1375

Human Primary Renal Epithelial Cells

T4090 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Bladder Epithelial Cells

T4940 5x10^5 cells / 1.0 ml Ask for price

GFP-Human Corneal Epithelial Cells

cAP-0211GFP 1Frozen Vial
EUR 841.5

Human Choroid Plexus Epithelial Cells

ABC-TC3568 1 vial Ask for price
Description: The choroid plexus epithelial cells (CPEpiC) are the structural basis, form a selective barrier between the nervous system and the blood, and secrete cerebrospinal fluid into the ventricles of the brain. CPEpiC express inward-rectifying anion channels which are thought to have an important role in the secretion of cerebrospinal fluid. CPEpiC synthesize and secrete many growth factors and other peptides that are of trophic benefit following injury to regions of the cerebroventricular system. For example, several growth factors are upregulated in choroid plexus after ischemic and traumatic insults to the central nervous system. The presence of numerous types of growth factor receptors in choroid plexus allows growth factor mediation of recovery processes by autocrine and paracrine mechanisms. CPEpiC culture has been widely used to evaluate their morphological and physiological properties, and its capacity to serve as an in vitro model to access the cellular mechanisms of organic cation transport across the cerebrospinal fluid-blood barrier.HCPEpiC from Gentaur Research Laboratories are isolated from human choroid plexus. HCPEpiC are cryopreserved after purification and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HCPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCPEpiC are guaranteed to further expand for 15 population doublings in the conditions specified by Gentaur Research Laboratories.

Human Mammary Epithelial Cells, Female

ABC-TC3692 1 vial Ask for price
Description: Human Mammary Epithelial Cells, when grown in LIMam Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HMEC include the stages of breast cancer development, three dimensional culture and carcinogen screening.Cell Features:HMEC are cryopreserved as tertiary cells, e.g. cells are isolated from human mammary tissue and expanded three times in culture vessels before being harvested for cryopreservation.HMEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Human Renal Cortical Epithelial Cells

ABC-TC4242 1 vial Ask for price
Description: Renal epithelial cells (REpiC) play a crucial role in renal function. Similar to most other epithelial cells, renal epithelial cells display a polarized morphology which is essential for their function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. Human REpiC are capable of internalizing bacteria. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. REpiC can produce inflammatory mediators such as cytokines and chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. REpiC express IL-2R alpha and MHC class II antigens during inflammation, indicating their capacity to participate in the pathogenesis of immune renal injury. To be able to study the relationship between renal epithelial cells and a variety of renal diseases, the HREpiC culture provides a useful in vitro model.HRCEpiC from Gentaur Research Laboratories are isolated from human kidneys. HRCEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HRCEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HRCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRCEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Fallopian Tube Epithelial Cells

ABC-TC5521 1 vial Ask for price
Description: Fallopian Tube Epithelial Cells have been isolated, plated and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest viability and plating efficiency. Fallopian Tube Cells are quality tested in our Medium to ensure optimal morphology and growth over a period of at least 5 population doublings.

Rat Primary Cervical Epithelial Cells

T4952 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Prostate Epithelial Cells

T4956 5x10^5 cells / 1.0 ml Ask for price

Human Primary Thymic Epithelial Cells

T4980 5x10^5 cells / 1.0 ml
EUR 975

Rat Primary Tracheal Epithelial Cells

T4804 5x10^5 cells / 1.0 ml Ask for price

Human Renal Cortical Epithelial Cells

cAP-0139 1Frozen Vial
EUR 693

Human Choroid Plexus Epithelial Cells.

cAP-0213 1Frozen Vial
EUR 445.5

Human Large Intestine Epithelial Cells

ABC-TC3667 1 vial Ask for price
Description: This cell line is available in limited quantities. Please contact us for more information.

Human Mammary Luminal Epithelial Cells

ABC-TC3695 1 vial Ask for price
Description: Human Mammary Luminal Epithelial Cell

Human Ovarian Surface Epithelial Cells

ABC-TC3743 1 vial Ask for price
Description: The human ovary is covered with a single layer of flat to cuboidal surface epithelial cells (OSE). OSE are physiologically involved in follicular rupture and the subsequent repair of the follicle wall during reproductive age. OSE exhibit a gland formation in coculture with endometrial stromal cells in an estrogen-rich environment. The phenotypic plasticity of these cells shares a mesenchymal property when they are cultured on two layers of extracellular matrix and collagen gel. OSE are the source of epithelial ovarian carcinomas. Understanding the mechanisms that regulate OSE cell survival, apoptosis, and cell growth is of great clinical importance because an aberrant signaling of any of these pathways is likely to be involved in epithelial ovarian cancer.HOSEpiC from Gentaur Research Laboratories are isolated from human ovarian tissue. HOSEpiC are cryopreserved either at primary or passage one cultures and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HOSEpiC are characterized by immunofluorescent method with cytokeratine antibodies (CK-14, -18 and -19). HOSEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOSEpiC are guaranteed to further expand for over 5 population doublings in the conditions provided by Gentaur Research Laboratories.

Human Retinal Pigment Epithelial Cells

ABC-TC3791 1 vial Ask for price
Description: The human retina is a multi-layered structure lining the rear of the eye. It consists of photoreceptor cells and retinal pigment epithelial cells (RPE). RPE situated between the neurosensory retina and the choroid, form the outer blood-retina barrier that controls the chemical composition of the subretinal space. RPE has long been recognized to differ from many other epithelia in the polarity of a number of proteins and whereas almost all other epithelia face an apical lumen devoid of matrix, the RPE apical plasma membrane is in contact with the extracellular matrix. During early embryonic development, RPE spontaneously transdifferentiate into neural retinal tissue. RPE play a critical role in the development and maintenance of adjacent photoreceptors. They generate a number of reactive oxygen species when illuminated with light and their proliferation is an important step in the pathogenesis of ocular diseases such as proliferative retinopathy. HRPEpiC from Gentaur Research Laboratories are isolated from human retina. HRPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HRPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HRPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRPEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Seminal Vesicle Epithelial Cells

ABC-TC3795 1 vial Ask for price
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Gentaur complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.

Human Small Intestine Epithelial Cells

ABC-TC3814 1 vial Ask for price
Description: Human small intestines cells are derived from whole small intestines that have been dissociated into multi-cellular aggregates and single cells then frozen. Human whole small intestines cells are from a single donor. The small intestine consists of several specialized epithelial cells that could help researchers determine the absorption potential of drug candidates. In addition, these cells can be used with studies of transport and metabolism of drugs and dietary chemicals.

Human Lobar Bronchial Epithelial Cells

ABC-TC3881 1 vial Ask for price
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Gentaur complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.

Human Renal Medullary Epithelial Cells

ABC-TC4244 1 vial Ask for price
Description: Normal Human Renal Epithelial Cells, when grown in LIRen Medium, provide an ideal low-serum culture model for the study of renal function, metabolism, nephrotoxicity or cancer research. Renal Nedullary Epithelial Cells are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our Renal Epithelial Cells are quality tested in LIRen Medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium. Cell Features:Mixed Renal Epithelial, Renal Cortical Epithelial, and Renal Medullary Epithelial are cryopreserved as primary cells isolated from human kidney tissue and expanded in culture vessels once before cryopreservation.Renal Proximal Tubule Epithelial are cryopreserved as secondary cells isolated from human kidney tissue and expanded in culture vessels twice before cryopreservation.All Renal Epithelial Cell types can be grown in a 0.5% serum medium without phenol red or antimicrobials when cultured in LIRen Medium.All Renal Epithelial Cell types are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Human Primary Mammary Epithelial Cells

T4036 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Corneal Epithelial Cells

T4058 5x10^5 cells / 1.0 ml
EUR 1475

Mouse Primary Corneal Epithelial Cells

T4571 5x10^5 cells / 1.0 ml
EUR 1375

Mouse Primary Bladder Epithelial Cells

T4640 5x10^5 cells / 1.0 ml
EUR 975

Rat Primary Bronchial Epithelial Cells

T4803 5x10^5 cells / 1.0 ml Ask for price

Human Primary Bladder Epithelial Cells

T4161 5x10^5 cells / 1.0 ml Ask for price

Human Retinal Pigment Epithelial Cells

cAP-0037 1Frozen Vial
EUR 594

Human Hepatic Biliary Epithelial Cells

cAP-0112 1Frozen Vial
EUR 594

Human Renal Medullary Epithelial Cells

cAP-0138 1Frozen Vial
EUR 693

Human Large Intestine Epithelial Cells

CSC-8241W One Frozen vial Ask for price

Human Colon Carcinoma Epithelial Cells

CSC-C4053X One Frozen vial Ask for price

Human Ovarian Surface Epithelial Cells

CSC-C9227J One Frozen vial Ask for price

Human Retinal Pigment Epithelial Cells

ALHE26 1ml frozen Vial
EUR 620

Human Small Intestine Epithelial Cells

ALHE42 1ml frozen Vial
EUR 620

Human Placental Epithelial Cells, Foetal

ABC-TC3754 1 vial Ask for price
Description: Human Placental Epithelial Cells (HPIEpC) are isolated from normal human amnion. They are cryopreserved at the end of primary culture. These epithelial cells proliferate well when they are thawed and plated from cryopreservation.

Rat Pulmonary Alveolar Epithelial Cells

ABC-TC4200 1 vial Ask for price
Description: Pulmonary alveolar epithelial cells (PAEpiC) comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover 95%.Rat Pulmonary alveolar epithelial cells are isolated from tissue of pathogen-free laboratory Sprague–Dawley (SD). They are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Human Primary Tracheal Epithelial Cells

T4007 5x10^5 cells / 1.0 ml
EUR 625

Rat Primary Esophageal Epithelial Cells

T4912 5x10^5 cells / 1.0 ml Ask for price

Human Primary Prostate Epithelial Cells

T4078 5x10^5 cells / 1.0 ml
EUR 625

Mouse Primary Tracheal Epithelial Cells

T4502 5x10^5 cells / 1.0 ml Ask for price

Mouse Primary Cervical Epithelial Cells

T4650 5x10^5 cells / 1.0 ml Ask for price

Mouse Primary Prostate Epithelial Cells

T4654 5x10^5 cells / 1.0 ml Ask for price

Human Primary Amniotic Epithelial Cells

T4109 5x10^5 cells / 1.0 ml
EUR 625

Human Small Intestinal Epithelial Cells

CSC-C4359X One Frozen vial Ask for price

Primary Human Prostate Epithelial Cells

CSC-C9039J One Frozen vial Ask for price

FIBROBLASTS, Antibody

GWB-4390F9 0.1 mg Ask for price

Fibroblasts Antibody

GWB-833798 0.1 mg Ask for price

CD-1 Mouse Kidney Epithelial Cells

ABC-TC3286 1 vial Ask for price
Description: Mouse primary kidney epithelial cells (MPKIDEpiCs) are isolated from tissue of pathogen-free laboratory mice. MPKIDEpiCs are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium for 3-7 days.

BALB/c Mouse Liver Epithelial Cells

ABC-TC3082 1 vial Ask for price
Description: BALB/c Mouse Primary Liver Epithelial Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Liver Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Liver Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Human Primary Bronchial Epithelial Cells

T4006 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Placental Epithelial Cells

T4076 5x10^5 cells / 1.0 ml
EUR 625

Immortalized Human Lung Epithelial Cells

T0488 1x106 cells / 1.0 ml Ask for price

C57BL/6 Mouse Liver Epithelial Cells

ABC-TC3206 1 vial Ask for price
Description: C57BL/6 Mouse Primary Liver Epithelial Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Liver Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.Mouse Primary Liver Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

BALB/c Mouse Dermal Epithelial Cells

ABC-TC3067 1 vial Ask for price
Description: BALB/c Mouse Primary Dermal Epithelial Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Dermal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Dermal Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

La myostatine régule la manufacturing du facteur de croissance 23 des fibroblastes (FGF23) dans les cellules de kind ostéoblaste UMR106 

Myostatin is a signaling molecule produced by skeletal muscle cells (myokine) that inhibits muscle hypertrophy and has additional paracrine and endocrine results in different organs together with bone. Myostatin binds to activin receptor kind 2B which varieties a posh with remodeling development factor-β kind I receptor (TGF-βRI) and induces intracellular p38MAPK and NFκB signaling. Fibroblast development issue 23 (FGF23) is a paracrine and endocrine mediator produced by bone cells and regulates phosphate and vitamin D metabolism within the kidney. P38MAPK and NFκB-dependent store-operated Ca2+ entry (SOCE) are optimistic regulators of FGF23 manufacturing.

Right here, we explored whether or not myostatin influences the synthesis of FGF23. Fgf23 gene expression was decided by qRT-PCR and FGF23 protein by ELISA in UMR106 osteoblast-like cells. UMR106 cells expressed activin receptor kind 2A and B. Myostatin upregulated Fgf23 gene expression and protein manufacturing. The myostatin impact on Fgf23 was considerably attenuated by TGF-βRI inhibitor SB431542, p38MAPK inhibitor SB202190, and NFκB inhibitor withaferin A. Furthermore, SOCE inhibitor 2-APB blunted the myostatin impact on Fgf23. Taken collectively, myostatin is a stimulator of Fgf23 expression in UMR106 cells, an impact not less than partially mediated by downstream TGF-βRI/p38MAPK signaling in addition to NFκB-dependent SOCE.

La diaphonie desmoplasique dans l’adénocarcinome canalaire pancréatique est reflétée par différentes réponses des lignées cellulaires Panc-1, MIAPaCa-2, PaTu-8902 et CAPAN-2 aux fibroblastes normaux associés au most cancers 

Background/intention: Pancreatic ductal adenocarcinoma (PDAC) nonetheless represents one of the vital aggressive cancers. Understanding of the epithelial-mesenchymal crosstalk as a vital a part of the tumor microenvironment ought to pave the best way for therapies to enhance affected person survival charges. Nicely-established cell strains current a helpful and reproducible mannequin to review PDAC biology. Nonetheless, the tumor-stromal interactions between most cancers cells and cancer-associated fibroblasts (CAFs) are nonetheless poorly understood.

Supplies and strategies: We studied interactions between 4 PDAC cell strains (Panc-1, CAPAN-2, MIAPaCa-2, and PaTu-8902) and conditioned media derived from major cultures of regular fibroblasts/PDAC-derived CAFs (PANFs).

Outcomes: When the examined PDAC cell strains have been stimulated by PANF-derived conditioned media, probably the most aggressive habits was acquired by the Panc-1 cell line (elevated quantity and dimension of colonies, remaining expression of vimentin and keratin eight in addition to improve of epithelial-to-mesenchymal polarization markers), whereas PaTu-8902 cells have been somewhat inhibited. Of be aware, administration of the conditioned media to MIAPaCa-2 cells resulted in an inverse impact on the dimensions and variety of colonies, whereas CAPAN-2 cells have been somewhat stimulated.

To clarify the heterogeneous sample of the noticed PDAC crosstalk on the in vitro stage, we additional in contrast the phenotype of major cultures of cells derived from ascitic fluid with that of the examined PDAC cell strains, analyzed tumor samples of PDAC sufferers, and carried out gene expression profiling of PANFs. Immuno-cyto/histo-chemical evaluation discovered particular phenotype variations inside the group of examined sufferers and examined PDAC cell strains, whereas the genomic strategy in PANFs discovered the important thing molecules (IL6, IL8, MFGE8 and periostin) which will contribute to the most cancers aggressive habits.

Conclusion: The desmoplastic patient-specific regulation of most cancers cells by CAFs (additionally demonstrated by the heterogeneous response of PDAC cell strains to fibroblasts) precludes easy focusing on and growth of an efficient remedy technique and somewhat requires institution of an individualized tumor-specific remedy protocol.

 

Analyse protéomique quantitative sans étiquette des vésicules extracellulaires libérées par des fibroblastes provenant de sufferers atteints d’amyotrophie spinale

 

Spinal muscular atrophy (SMA) is an autosomal recessive dysfunction that represents a big reason for toddler mortality. SMA is characterised by decreased ranges of the Survival Motor Neuron protein resulting in the lack of alpha motor neurons within the spinal wire and mind stem in addition to defects in peripheral tissues equivalent to skeletal muscle and liver.

With progress in promising therapies equivalent to antisense oligonucleotide and gene alternative, there stays a necessity to raised perceive illness subtypes and develop biomarkers for improved diagnostics and therapeutic monitoring. On this examine, we’ve got examined the utility of extracellular vesicles as a supply of biomarker discovery in patient-derived fibroblast cells. Proteome examination using data-independent acquisition and ion mobility mass spectrometry recognized 684 protein teams current in all organic replicates examined.

Label-free quantitative evaluation recognized 116 statistically important protein alterations in comparison with management cells, together with a number of identified SMA biomarkers. Protein stage variations have been additionally noticed in regulators of Wnt signaling and Cajal our bodies. Lastly, ranges of insulin development issue binding protein-Three was validated as being considerably greater in extracellular vesicles remoted from SMA cells.

We conclude that extracellular vesicles signify a promising supply for SMA biomarker discovery in addition to a related constituent for advancing our understanding of SMA pathophysiology. This text is protected by copyright. All rights reserved.

 

Sos1 module la synthèse, la prolifération et la migration de la matrice extracellulaire dans les fibroblastes 

Non-reversible fibrosis is frequent in varied illnesses equivalent to persistent renal failure, liver cirrhosis, persistent pancreatitis, pulmonary fibrosis, rheumatoid arthritis and atherosclerosis. Reworking development issue beta 1 (TGF-β1) is concerned in nearly all varieties of fibrosis. We beforehand described the involvement of Ras GTPase isoforms within the regulation of TGF-β1-induced fibrosis.

The guanine nucleotide trade issue Son of Sevenless (Sos) is the principle Ras activator, however the position of the ubiquitously expressed Sos1 within the growth of fibrosis has not been studied. For this function, we remoted and cultured Sos1 knock-out (KO) mouse embryonic fibroblasts, the principle extracellular matrix proteins (ECM)-producing cells, and we analyzed ECM synthesis, cell proliferation and migration within the absence of Sos1, in addition to the position of the principle Sos1-Ras effectors, Erk1/2 and Akt, in these processes.

The absence of Sos1 will increase collagen I expression (by the PI3K-Akt signaling pathway), complete collagen proteins, and barely will increase fibronectin expression; Sos1 regulates fibroblast proliferation by each PI3K-Akt and Raf-Erk pathways, and Sos1-PI3K-Akt signaling regulates fibroblast migration.

These examine exhibits that Sos1 regulates ECM synthesis and migration (by Ras-PI3K-Akt) and proliferation (by Ras-PI3K-Akt and Ras-Raf-Erk) in fibroblasts, and describe for the primary time the position of the Sos1-Ras signaling axis within the regulation of mobile processes concerned within the growth of fibrosis.

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