Le knockdown de Fus inhibe l’effet profibrogène

Le knockdown de Fus inhibe l’effet profibrogène

| Coryne

Le knockdown de Fus inhibe l’effet profibrogène des fibroblastes cardiaques induits par l’angiotensine II en ciblant Pax3, régulant ainsi la voie TGF- / 1 / Smad

 The Angiotensin II/transforming growth factor-β1 (AngII/TGF-β1) signal axis is an important regulatory pathway for atrial fibrosis, which can contribute to atrial fibrillation (AF). Fused in sarcoma (FUS) was recently found to regulate cardiac diseases. This study aimed to investigate whether FUS could regulate AngII induced fibrosis and uncover the possible mechanisms. The expression of FUS in AF patients and AngII-induced cardiac fibroblasts was measured by RT-qPCR and western blot assays. Fus was silenced in cells using short hairpin RNA (shRNA), then cell proliferation, migration, collagen synthesis and TGF-β1/Smad signaling were detected by CCK-8, wound healing and western blot assays, respectively. The possible target for Fus was predicted by searching Starbase database and verified by RNA-binding protein immunoprecipitation (RIP) and RNA pull down.

Cells were overexpressed with Pax3 in the presence of Fus silence and AngII stimulation, then the above cellular processes were further evaluated. Results showed that FUS was upregulated in AF patients and AngII-induced cardiac fibroblasts. Fus knockdown inhibited AngII-enhanced cell proliferation, migration, collagen synthesis and TGF-β1/Smad signaling activation. Furthermore, Fus functions as an RNA-binding protein to bind to Pax3 mRNA and positively regulate its expression. Further studies demonstrated that Pax3 overexpression canceled the above effects of Fus knockdown on cell proliferation, migration, collagen synthesis, and TGF-β1/Smad signaling activation in AngII-induced cells. In conclusion, Fus could target Pax3 to increase the pro-fibrotic effect of AngII in cardiac fibroblasts via activating TGF-β1/Smad signaling. Knockdown of Fus/Pax3 axis may provide a potential therapy for relieving AF.

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Fibroblasts/Epithelial Cells Antibody (PE)
abx413038-200l 200 µl
EUR 250

Fibroblasts
MBS6507500-01mg 0.1mg
EUR 790

Fibroblasts
MBS6507500-5x01mg 5x0.1mg
EUR 3395

Fibroblasts/Epithelial Cells Antibody (RPE)
abx413037-100tests 100 tests
EUR 811.2

Fibroblasts/Epithelial Cells Antibody (RPE)
abx413038-25tests 25 tests
EUR 427.2

Fibroblasts / Epithelial Cells Antibody (RPE)
GWB-2B065D 100 TESTS Ask for price

Fibroblasts / Epithelial Cells Antibody (RPE)
GWB-F0EF95 25 TESTS Ask for price

OASA02809-25UG - FIBROBLASTS/EPITHELIAL CELLS Antibody
OASA02809-25UG 25ug
EUR 149

OASA02808-200UG - FIBROBLASTS/EPITHELIAL CELLS Antibody
OASA02808-200UG 0.2mg
EUR 499

Rat Lung Fibroblasts
ABC-TC4158 1 vial Ask for price
Description: Rat Primary Lung Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Lung Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Lung Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Skin Fibroblasts
ABC-TC4217 1 vial Ask for price
Description: Rat skin fibroblasts, 2-week Wistar rat

Rat Vein Fibroblasts
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Description: Rat Primary Vein Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Vein Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Vein Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat liver fibroblasts
ABC-TC4153 1 vial Ask for price
Description: Rat liver fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Mouse liver fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Dog liver Fibroblasts
cAP-d0010 1Frozen Vial
EUR 396

Mouse Lung Fibroblasts
ABC-TC3931 1 vial Ask for price
Description: Mouse lung fibroblasts, 2-week ICR mouse

Rat Aortic Fibroblasts
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Description: Rat Primary Aortic Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Aortic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Aortic Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Artery Fibroblasts
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Description: Rat Primary Artery Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Dermal Fibroblasts
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Description: Rat Dermal Fibroblasts (RDF) are derived from the dermis of normal Sprague Dawley rats. They are cryopreserved at first passage and can be cultured and propagated at least 8 population doublings.

Rat Kidney Fibroblasts
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Description: Rat kidney fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Rat kidney fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Rat Spleen Fibroblasts
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Description: Rat Primary Spleen Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Spleen Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Spleen Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Uterus Fibroblasts
ABC-TC4237 1 vial Ask for price
Description: Rat uterus fibroblasts, 6-week Wistar rat

Human Skin Fibroblasts
HXXFB-00001 1*10^6/vial
EUR 1529

Human Liver Fibroblasts
ABC-TC3672 1 vial Ask for price
Description: Human Liver Fibroblasts are isolated from normal human liver tissue. Human Liver Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml.

Mouse Aorta Fibroblasts
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Description: Mouse aorta fibroblasts, 2-week ICR mouse

Rabbit Lung Fibroblasts
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Description: Rabbit Primary Lung Fibroblasts from Gentaur are isolated from lung tissue of New Zealand White Rabbits. Rabbit Primary Lung Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rabbit Primary Lung Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rat bladder fibroblasts
ABC-TC4080 1 vial Ask for price
Description: Rat bladder fibroblasts, 3-week Wistar rat

Rat Cardiac Fibroblasts
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Description: Rat Primary Cardiac Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Cardiac Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Cardiac Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Mammary Fibroblasts
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Description: Rat Primary Mammary Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Mammary Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Mammary Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Ovarian Fibroblasts
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Description: Rat Primary Ovarian Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Ovarian Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Ovarian Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Thyroid Fibroblasts
ABC-TC4232 1 vial Ask for price
Description: Rat Primary Thyroid Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Thyroid Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Thyroid Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Cardiac Fibroblasts
CP-R074 5×105 cells/vial
EUR 550
Description: Rat

Human Fibroblasts, Dermis
ABC-TC3622 1 vial Ask for price
Description: The HFIB-D cultures are produced on the base of human tissues. The HFIB-D are isolated according to known procedures. Then primary cultures are produced. After further culturing, the HFIB-D are frozen in a computer controlled freezing unit.

Human Tonsil Fibroblasts
ABC-TC3835 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Fibroblasts are among the most accessible normal mammalian cell types and still the most amenable to culture in vitro.HTF from Gentaur Research Laboratories are isolated from human tonsils. HTF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HTF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HTF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Monkey Liver Fibroblasts
ABC-TC3908 1 vial Ask for price
Description: Monkey Primary Liver Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Liver Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Liver Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rat Prostate Fibroblasts
ABC-TC4198 1 vial Ask for price
Description: Rat Primary Prostate Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Prostate Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Prostate Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Tracheal Fibroblasts
ABC-TC4234 1 vial Ask for price
Description: Rat Primary Tracheal Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Tracheal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Tracheal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Vascular Fibroblasts
ABC-TC4238 1 vial Ask for price
Description: Rat vascular fibroblasts, 2-week Wistar rat

Human Kidney Fibroblasts
ABC-TC5519 1 vial Ask for price
Description: Human Kidney Fibroblasts are isolated from the human adult healthy renal tissue. HKF-a are cryopreserved after passage one culture and delivered frozen. HKF-a are guaranteed to further expand for 15 population doublings under the conditions provided by in the technical sheet.As an excretory organ in the urinary system, the kidney greatly influences an individual’s well being by preserving homeostasis through regulating electrolytes, pH, and blood pressure among other control mechanisms. Comprising the majority of interstitial cells in the kidney, renal fibroblasts are responsible for the synthesis of collagen I of the extracellular matrix. Also, renal cortical fibroblasts synthesize erythropoietin, an important glycoprotein hormone that controls the production of red blood cells and renal medullary fibroblasts produce prostaglandins, a class of autocrine or paracrine hormones, which help maintain water and electrolyte homeostasis. Renal interstitial fibrosis, a disorder that may escalate to chronic kidney disease, is often correlated with excessive deposition of extracellular matrix by renal fibroblasts, the regulation of which process is under intensive research.

Human Kidney Fibroblasts
CSC-8240W One Frozen vial Ask for price

Human Dermal Fibroblasts
CSC-C0519Z One Frozen vial Ask for price

Human Spleen Fibroblasts
CSC-C4868L One Frozen vial Ask for price

Native Human Fibroblasts
MBS556459-INQUIRE INQUIRE Ask for price

Human Adrenal Fibroblasts
ABC-TC3478 1 vial Ask for price
Description: The adrenal gland plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones. Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.HAdF from Gentaur Research Laboratories are isolated from human adrenal tissue. HAdF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HAdF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HAdF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAdF are guaranteed to further expand for 15 population doublings under the conditions provided by Gentaur Research Laboratories.

Human Cardiac Fibroblasts
ABC-TC3533 1 vial Ask for price
Description: The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors.HCF from Gentaur Research Laboratories are isolated from the human heart. HCF-a are cryopreserved at secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCF-a are characterized by immunofluorescent method with antibody to fibronectin. HCF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-a are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Mammary Fibroblasts
ABC-TC3694 1 vial Ask for price
Description: Fibroblasts are the most common types of mammary stromal cells. Mammary stromal cells differentiate into fibroblasts, adipocytes or vascular structures in a hormone- and substatum-dependent manner, and may explain the dramatic changes in stromal composition during both normal mammary gland development and tumorigenesis. The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. The Mammary stromal fibroblasts express and produce cytokines such as SF witch plays roles in mammary cancer. HMF from Gentaur Research Laboratories are isolated from human mammary tissue. HMF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HMF are characterized by immunofluorescent method with antibodies to fibronectin, CD90 and vimentin. HMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMF are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

Human Ovarian Fibroblasts
ABC-TC3741 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Ovarian fibroblasts stimulate human ovarian cancer cell invasion and expression of 72-kDa gelatinase A (MMP-2).HOF from Gentaur Research Laboratories are isolated from human ovary tissue. HOF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HOF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HOF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Splenic Fibroblasts
ABC-TC3818 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. There is good evidence that fibroblasts in different parts of the body are intrinsically different and display different regulatory functions on surrounding cells. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. HSF from Gentaur Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Thyroid Fibroblasts
ABC-TC3832 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Because the thyroid is easily susceptible to inflammatory autoimmune diseases that can result in tissue damage, thyroid fibroblasts may play an important role in regulating thyroid function and reaction to disease. HThF from Gentaur Research Laboratories are isolated from human thyroid tissue. HThF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HThF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HThF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HThF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Uterine Fibroblasts
ABC-TC3863 1 vial Ask for price
Description: Derived from the mesoderm, connective tissue is involved in maintaining the structural integrity of the body. The predominant cells found in connective tissue are fibroblasts, which continuously secrete various components of the extracellular matrix. Depending on the site of origin and physiological status, fibroblasts may exhibit different morphological phenotypes and various functional properties.Gentaur offers a range of Human Fibroblasts produced at Gentaur's cell culture facility. The cells are isolated from normal human juvenile foreskin or adult skin from different locations. In addition, Gentaur offers Fibroblasts from adult lung tissue, aortic adventitial tissue, and villous placental tissue.Shortly after isolation, all Gentaur Human Fibroblasts are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Monkey Aortic Fibroblasts
ABC-TC3903 1 vial Ask for price
Description: Monkey Primary Aortic Fibroblasts from Gentaur are isolated from aortic tissue of Cynomolgus Monkey. Monkey Primary Aortic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Monkey Dermal Fibroblasts
ABC-TC3905 1 vial Ask for price
Description: Monkey Primary Dermal Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Dermal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rabbit Aortic Fibroblasts
ABC-TC4055 1 vial Ask for price
Description: Rabbit Primary Aortic Fibroblasts from Gentaur are isolated from aortic tissue of New Zealand White Rabbits. Rabbit Primary Aortic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rabbit Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rat Embryonic Fibroblasts
ABC-TC4128 1 vial Ask for price
Description: Rat primary embryonic fibroblasts are isolated from day 13.5 embryos of Sprague-Dawley rats. Rat primary embryonic fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Rat Lymphatic Fibroblasts
ABC-TC4164 1 vial Ask for price
Description: Rat Primary Lymphatic Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Lymphatic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Lymphatic Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Pulmonary Fibroblasts
ABC-TC4204 1 vial Ask for price
Description: The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions.

Human Gastric Fibroblasts
ABC-TC5516 1 vial Ask for price
Description: Gastric fibroblasts (GF), which are located in the stomach, are mesenchymal cells derived from the embryonic mesoderm. Fibroblasts secrete a non-rigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. Studies in vitro and in vivo show that GF can regulate epithelial regeneration by influencing epithelial cell proliferation and differentiation. In addition to their role in wound healing, human GF (HGF) have been shown to be key contributors in the development and progression of gastric carcinoma. HGF are a useful model for examining the underlying mechanisms that cause gastric carcinoma and may provide novel insights for developing new cancer drug therapies.

Human Splenic Fibroblasts 
CSC-C9230J One Frozen vial Ask for price

Mouse Cardiac Fibroblasts
CP-M074 5×105 cells/vial
EUR 580
Description: Mouse

Human Thyroid Fibroblasts 
CSC-7702W One Frozen vial Ask for price

Human Colonic Fibroblasts
CSC-8242W One Frozen vial Ask for price

Human Hepatic Fibroblasts
CSC-C1500 One Frozen vial Ask for price

Human Cardiac Fibroblasts
CSC-C1503 One Frozen vial Ask for price

Human Mammary Fibroblasts
CSC-C1527 One Frozen vial Ask for price

Porcine Aortic Fibroblasts
ABC-HP020X 1 vial Ask for price
Description: Porcine Aortic Fibroblasts are isolated from aorta of porcine. Porcine Aortic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials.

Human Gingival Fibroblasts
ABC-TC3627 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells with many vital functions during development and in adult organisms. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Fibroblasts are among the most accessible normal mammalian cell types and still the most amenable to culture in vitro. Gingival fibroblasts are the major constituents of gingival tissue and play a key role in their health maintenance. Human gingival fibroblasts (HGnF) express a wide surface molecular panel including mainly CD9, CD26, CD55, CD59, CD63, CD71, CD86 CD95, CD99 and CD117. They were also demonstrated to express mRNAs for protease-activated receptor-1 (PAR-1) and PAR-3.HGnF from Gentaur Research Laboratories are isolated from human gingiva. HGnF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HGnF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HGnF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGnF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Prostate Fibroblasts
ABC-TC3765 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of the easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Cultured prostate fibroblasts synthesize FGF-like growth factor that stimulate their growth and is believed to be important in the development of benign prostatic hyperplasia.HPrF from Gentaur Research Laboratories are isolated from human prostate tissue. HPrF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPrF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HPrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPrF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Monkey Cardiac Fibroblasts
ABC-TC3904 1 vial Ask for price
Description: Monkey Primary Cardiac Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Cardiac Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Cardiac Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rat Intestinal Fibroblasts
ABC-TC4141 1 vial Ask for price
Description: Rat Primary Intestinal Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Intestinal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Intestinal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Perineural Fibroblasts
ABC-TC4191 1 vial Ask for price
Description: Perineurial cells are of mesenchymal origin. They make up the perineurium, which has the important role of maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier.

Rabbit Cardiac Fibroblasts
ABC-TC5512 1 vial Ask for price
Description: Rabbit Primary Cardiac Fibroblasts from Gentaur are isolated from heart tissue of New Zealand White Rabbit. Rabbit Primary Cardiac Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen.

Human Prostate Fibroblasts
ALHE28 1ml frozen Vial
EUR 620

Human Prostate Fibroblasts 
CSC-7730W One Frozen vial Ask for price

Human Gingival Fibroblasts
cAP-0140 1Frozen Vial
EUR 693

Human pancreas fibroblasts
P0018025 One Frozen vial
EUR 580

Human Bronchial Fibroblasts
ABC-TC3529 1 vial Ask for price
Description: Human Bronchial Fibroblasts from Gentaur Research Laboratories are isolated from human bronchus tissue. HBF are cryopreserved at secondary culture (P1) and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBF are characterized by immunofluorescent method with antibody to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Lymphatic Fibroblasts
ABC-TC3688 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Besides, dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.HLF from Gentaur Research Laboratories are isolated from human lymph nodes. HLF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Pulmonary Fibroblasts
ABC-TC3776 1 vial Ask for price
Description: The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. HPF from Gentaur Research Laboratories are isolated from human lung tissue. HPF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPF are characterized by immunofluorescent method with antibody to fibronectin. HPF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Rabbit Vascular Fibroblasts
ABC-TC4066 1 vial Ask for price
Description: Rabbit vascular fibroblasts, 5-week rabbit

Human Intestine Fibroblasts
cAP-0206 1Frozen Vial
EUR 544.5

Human Esophageal Fibroblasts
ABC-TC3613 1 vial Ask for price
Description: Esophageal fibroblasts which line the lamina propria of the upper and lower esophagus are mesenchymal cells that originated from the embryonic mesoderm. They have been shown to play an important role in angiogenesis through TGF-β and VEGF signaling pathways in the highly angiogenic esophageal squamous cell carcinoma. Predominantly, fibroblasts secrete a nonrigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in the connective tissues and play major roles in wound healing. Because they are one of the simplest types of cells to grow in culture fibroblasts have been extensively used for a wide range of cellular and molecular studies. In addition, fibroblasts’ durability makes them amenable to a wide variety of manipulations ranging from gene transfection to microinjection.The HEF cells from Gentaur Research Laboratories are isolated from human esophageal tissue. HEF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEF are characterized by immunofluorescence with antibody specific to fibronectin. HEF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEF are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

Human Intestinal Fibroblasts
ABC-TC3655 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.HIF from Gentaur Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Monkey Lymphatic Fibroblasts
ABC-TC3910 1 vial Ask for price
Description: Monkey Primary Lymphatic Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Lymphatic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Lymphatic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Mouse Intestinal Fibroblasts
ABC-TC3929 1 vial Ask for price
Description: Mouse intestinal fibroblasts, 2-week C57B mouse

Human Pancreatic Fibroblasts
ABC-TC5470 1 vial Ask for price
Description: Human Primary Pancreatic Fibroblasts are isolated from normal human pancreatic tissue. Human Primary Pancreatic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml.

Human Pancreatic Fibroblasts
CSC-C4882L One Frozen vial Ask for price

Human Pancreatic Fibroblasts
cAP-0144 1Frozen Vial
EUR 693

Fibroblasts, Epithelial Cells
MBS609129-02mg 0.2mg
EUR 820

Fibroblasts, Epithelial Cells
MBS609129-5x02mg 5x0.2mg
EUR 3550

Human Gallbladder Fibroblasts
ABC-TC3625 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. Because they are one of the easiest types of cells to grow in culture, they have been extensively used for a wide range of cellular and molecular studies. Additionally, their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Human gallbladder fibroblasts (HGBF) are responsible for eicosanoid synthesis, specifically PGI2. Chronic stimulation of HGBF results in up-regulated synthesis and release of PGI2 which aids in formation of early acute cholecystitis. HGBF are very versatile cells and could be used in studies of many conditions, such as the early stages of acute cholecystitis.HGBF from Gentaur Research Laboratories are isolated from human gallbladder tissue. HGBF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HGBF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HGBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGBF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Pericardial Fibroblasts
ABC-TC3748 1 vial Ask for price
Description: Pericardial Fibroblasts are the principal cell type of the serous pericardium. They help to form the strong connective tissue that allows the pericardium to serve as a protective sac for the heart. Pericardial fibroblasts are exposed to a dynamic mechanical environment, being attached to the diaphragm, which influences the structure integrity of both healthy and healing tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen and there is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts have been extensively used for a wide range of cellular and molecular studies because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.HPcF from Gentaur Research Laboratories are isolated from the human heart. HPcF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPcF are characterized by immunofluorescent method with antibody to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPcF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Monkey Intestinal Fibroblasts
ABC-TC3906 1 vial Ask for price
Description: Monkey Primary Intestinal Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Intestinal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Intestinal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rabbit Intestinal Fibroblasts
ABC-TC4060 1 vial Ask for price
Description: Rabbit Primary Intestinal Fibroblasts from Gentaur are isolated from Intestinal tissue of New Zealand White Rabbits. Rabbit Primary Intestinal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rabbit Primary Intestinal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Human Parathyroid Fibroblasts
cAP-0218 each
EUR 782.1

Immortalized Human Fibroblasts
ABI-TC052D 1 vial Ask for price
Description: Fibroblasts are the most common cells of mammalian connective tissue. AcceGen offers an Immortalised fibroblast cell line originated from the human foreskin.

Human Conjunctival Fibroblasts
ABC-TC3574 1 vial Ask for price
Description: The fibroblast is the cellular element responsible for fibrosis. Conjunctival fibroblasts, as other fibroblasts, act not only as structural cells in the production of the matrix components, but are also actively involved as effectors in allergic conjunctivitis. Conjunctival fibroblasts cultured with histamine induce the production of proinflammatory cytokines, such as IL-1, IL-6 and IL-8. Conjunctival fibroblast culture can serve as a useful in vitro model for rapid screening of drugs for their effect on fibroblast morphology, motility, and proliferation, and provide a basis for the development of new treatments for a variety of ocular proliferative disorders.HConF from Gentaur Research Laboratories are isolated from human conjunctiva. HConF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HConF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HConF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HConF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Fibroblasts Depletion Services
fAP-0032 Service Ask for price

Rat Lung Fibroblasts, Adult
ABC-TC4159 1 vial Ask for price
Description: Rat Lung Fibroblasts (RLF) are derived from the lung of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RLF can be cultured and propagated 10 population doublings.

TIMP-2, human fibroblasts
7788-5 each
EUR 405.6

Human Lung Fibroblasts, Adult
ABC-TC3683 1 vial Ask for price
Description: Human Lung Fibroblasts (HLF) are derived from normal human lung parenchyma. They are cryopreserved at first passage and can be cultured and propagated at least 12 population doublings.

Human Skin Cells (Fibroblasts)
ABC-TC3807 1 vial Ask for price
Description: Human fibroblasts are derived from cultured skin explants. These fibroblasts are from a single donor.

Rat Lung Fibroblasts-Adult
ABC-TC4160 1 vial Ask for price
Description: Rat lung fibroblasts are isolated from tissue of adult Sprague-Dawley rats. Rat lung fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

GFP-Mouse Lung Fibroblasts
cAP-m0023GFP 1Frozen Vial
EUR 782.1

FBS, Fibroblasts Optimized I
F0691-050 500ml Ask for price

OASA02810-100T - Fibroblasts/Epithelial Cells Antibody - PE Conjugated
OASA02810-100T 100Tests
EUR 519

OASA02811-25T - Fibroblasts/Epithelial Cells Antibody - PE Conjugated
OASA02811-25T 25Tests
EUR 219

Rat Neonatal Lung Fibroblasts
Ab1004 1ml frozen Vial
EUR 0.2

Wistar Rat Dermal Fibroblasts
ABC-TC3400 1 vial Ask for price
Description: For routine cell cultivation, We recommends to work without antibiotics / antimycotics. For isolation, However, we recommend the use of antibiotics / antimycotics up to passage 2.

Human Lung Fibroblasts, Foetal
ABC-TC3684 1 vial Ask for price
Description: Human Lung Fibroblasts (HLF) are derived from normal human lung parenchyma. They are cryopreserved at first passage and can be cultured and propagated at least 12 population doublings.

Rat cardiac fibroblasts, Adult
ABC-TC4106 1 vial Ask for price
Description: Rat primary cardiac fibroblasts are isolated from heart tissue of adult Sprague-Dawley rats. Rat primary cardiac fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Rat Neonatal Lung Fibroblasts
cAP-r0008Lung 1Frozen Vial
EUR 396

Human Dermal Fibroblasts (HDFa)
P0020022 One Frozen vial
EUR 420

Rat Neonatal Lung Fibroblasts
R1020 1ml frozen Vial
EUR 0.2

Human Dermal Fibroblasts, Adult
ABC-TC3029 1 vial Ask for price
Description: Human Dermal Fibroblasts-Adult are isolated from adult human skin. Human Dermal Fibroblasts-Adult are cryopreserved at P0 and delivered frozen. Human Dermal Fibroblasts-Adult are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. Human Dermal Fibroblasts-Adult are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.Human Dermal Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency.

CD57 Mouse Cardiac Fibroblasts
ABC-TC3319 1 vial Ask for price
Description: CD57-Mouse Cardiac Fibroblasts

Human Dermal Fibroblasts, Fetal
ABC-TC3588 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. In addition, dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.HDF from Gentaur Research Laboratories are isolated from fetal human skin. HDF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HDF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HDF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HDF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Vas Deferens Fibroblasts
ABC-TC3867 1 vial Ask for price
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Gentaur complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.

Rat Brain Vascular Fibroblasts
ABC-TC4100 1 vial Ask for price
Description: Rat Primary Brain Vascular Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Brain Vascular Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Brain Vascular Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Dermal Fibroblasts - neonate
ABC-TC4120 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture.

Rat Dermal Fibroblasts-adult
ABC-TC4121 1 vial Ask for price
Description: Rat Primary Dermal Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Dermal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Dermal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Dermal Fibroblasts-fetal
ABC-TC4122 1 vial Ask for price
Description: Rat Primary Dermal Fibroblasts from Gentaur are isolated from tissue of Sprague-Dawley Rats. Rat Primary Dermal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Dermal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Pulmonary Vein Fibroblasts
ABC-TC4206 1 vial Ask for price
Description: Rat Primary Pulmonary Vein Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Pulmonary Vein Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Pulmonary Vein Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Human Dermal Fibroblasts (Adult)
cAP-0008-ad 1Frozen Vial
EUR 445.5

Mouse Liver Fibroblasts (C57BL6)
cAP-m0027 1Frozen Vial
EUR 396

Mouse Heart Fibroblasts (C57BL6)
cAP-m0028 1Frozen Vial
EUR 396

Mouse Anti Human Fibroblasts/Epithelial Cells Monoclonal Antibody,RPE
DMABT-49595MH 25 TEST
EUR 382.2
Description: Mouse

Rat Neonatal Dermal Fibroblasts
Ab1002 1ml frozen Vial
EUR 0.2

CF1 Mouse Embryonic Fibroblasts
ABC-TC3333 1 vial Ask for price
Description: CF1 Mouse Primary Embryonic Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Embryonic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.Mouse Primary Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Human Dermal Fibroblasts, Pooled
ABC-TC3399 1 vial Ask for price
Description: For routine cell cultivation, We recommends to work without antibiotics / antimycotics. For isolation, However, we recommend the use of antibiotics / antimycotics up to passage 2.

Rat Lung Fibroblasts-Neonatal
ABC-TC4161 1 vial Ask for price
Description: Rat lung fibroblasts (RLUFB) are isolated from tissue of 1-day-old neonatal Sprague-Dawley rats. Rat lung fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Human Lung Fibroblasts-Adults
cAP-0033Ad 1Frozen Vial
EUR 594

Rat Neonatal Dermal Fibroblasts
cAP-r0008Der 1Frozen Vial
EUR 396

Fibroblasts, Epithelial Cells (AP)
MBS6247938-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (AP)
MBS6247938-5x01mL 5x0.1mL
EUR 4815

ICR Mouse Embryonic Fibroblasts
MUIEF-01001 5*10^6/vial
EUR 219

Rat Neonatal Dermal Fibroblasts
R1019 1ml frozen Vial
EUR 0.2

CD57 Mouse Lymphatic Fibroblasts
ABC-TC3323 1 vial Ask for price
Description: CD57-Mouse Lymphatic Fibroblasts

Human Choroid Plexus Fibroblasts
ABC-TC3569 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells, which they derived from the embryonic mesoderm. Although cells loosely called fibroblasts have been isolated from many tissues and assumed to be connective tissue cells, fibroblasts appear to be the least specialized cells. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Fibroblasts are the easiest of cells to grow in culture-a feature that has made them a favorite subject for cell biological studies.HCPF from Gentaur Research Laboratories are isolated from human choroid pluxus. HCPF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCPF are characterized by immunofluorescent method with antibody to fibronectin. HCPF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCPF are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Lymphatic Fibroblasts Cell
ABC-TC3689 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies.

Human Ocular Choroid Fibroblasts
ABC-TC3729 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They are one of the easiest types of cells to grow in culture and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. During wound healing, fibroblasts switch from a migrator, repopulation phenotype to a contractile, matrix-reassembling phenotype. Choroid fibroblasts are exposed to a dynamic environment that influences cellular VEGF expression, which plays a significant role in angiogenesis and macular degeneration.HOCF from Gentaur Research Laboratories are isolated from human ocular choroid tissue. HOCF are cryopreserved at primary culture. Each vial contains 5×10^5 cells in 1 ml volume. HOCF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HOCF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOCF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Rat cardiac fibroblasts, Neonatal
ABC-TC4107 1 vial Ask for price
Description: Rat primary cardiac fibroblasts (RCAFC) are isolated from heart tissue of 1-day-old neonatal Sprague-Dawley rats. Rat primary cardiac fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Rat Pulmonary Artery Fibroblasts
ABC-TC4202 1 vial Ask for price
Description: Rat Primary Pulmonary Artery Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Pulmonary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Pulmonary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Small Intestinal Fibroblasts
ABC-TC5531 1 vial Ask for price
Description: Rat Intestinal Fibroblasts from Gentaur are isolated from small intestinal tissue of 1-day-old neonatal Sprague-Dawley rats. Rat Primary Intestinal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur Culture Complete Growth Medium generally for 2-5 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106 cells per ml and is delivered frozen.

Human Dermal Fibroblasts-fetal 
CSC-7797W One Frozen vial Ask for price

Rat Neonatal Cardiac Fibroblasts
cAP-r0008Cardiac 1Frozen Vial
EUR 396

Fibroblasts, Epithelial Cells (HRP)
MBS6250880-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (HRP)
MBS6250880-5x01mL 5x0.1mL
EUR 4815

Fibroblasts, Epithelial Cells (APC)
MBS6268791-01mL 0.1mL
EUR 1105

Fibroblasts, Epithelial Cells (APC)
MBS6268791-5x01mL 5x0.1mL
EUR 4815

Rat Neonatal Cardiac Fibroblasts
R1018 1ml frozen Vial
EUR 0.2

CD-1 Mouse Lung Fibroblasts
ABC-TC3292 1 vial Ask for price
Description: Mouse lung fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse lung fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

Human Dermal Fibroblasts, Neonatal
ABC-TC3451 1 vial Ask for price
Description: Human Dermal Fibroblasts-Neonatal are isolated form neonatal human skin. Human Dermal Fibroblasts-Neonatal are cryopreserved at P1 and delivered frozen. Human Dermal Fibroblasts-Neonatal are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. Human Dermal Fibroblasts-Neonatal are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

Human Bladder Stromal Fibroblasts
ABC-TC3515 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of the easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechanical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. As cells of mesenchymal origin, human bladder stromal fibroblasts (HBdSF) also secrete FGF-7, which interact primarily with bladder epithelial cells. FGF-7 secreted by HBdSF is essential for stimulating the formation of a multilayered stratified bladder epithelium. HBdSF from Gentaur Research Laboratories are isolated from human bladder tissue. HBdSF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBdSF are characterized by immunofluorescent method with antibody to fibronectin. HBdSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Rabbit Liver Vascular Fibroblasts
ABC-TC4062 1 vial Ask for price
Description: Rabbit liver vascular fibroblasts, 5-week rabbit

Rat Dermal Fibroblasts-neonatal
ABC-TC4123 1 vial Ask for price
Description: Rat Primary Dermal Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Dermal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Dermal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Human Dermal Fibroblasts (Neonatal)
cAP-0008-neo 1Frozen Vial
EUR 445.5

Fibroblasts, Epithelial Cells (FITC)
MBS6249884-01mL 0.1(mL
EUR 1105

Fibroblasts, Epithelial Cells (FITC)
MBS6249884-5x01mL 5x0.1mL
EUR 4815

Canine Primary Dermal Fibroblasts
T0269 5x10^5 cells / 1.0 ml Ask for price

Human Primary Uterine Fibroblasts
T4084 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Pulmonary Fibroblasts
T4801 5x10^5 cells / 1.0 ml Ask for price

L’acide salvianolique B inhibe la différenciation des myofibroblastes et l’accumulation de matrice extracellulaire dans les fibroblastes polypes nasaux via la voie de signalisation TGF-sign1 

In the process of nasal tissue remodeling, nasal fibroblasts serve an important role via myofibroblast differentiation and the production of extracellular matrix (ECM). Nasal fibroblast abnormalities can lead to conditions such as chronic rhinosinusitis. Salvianolic acid B (Sal B), a water-soluble active pharmaceutical compound extract from the root of the traditional Chinese medicine Salvia miltiorrhiza, displays antioxidative, antiproliferative and antifibrosis properties. The present study aimed to investigate the mechanism underlying the effects of Sal B on nasal polyp fibroblast (NPF) myofibroblast differentiation and ECM accumulation. Primary NPFs were obtained from nasal polyps of patients with chronic sinusitis. The proliferative and cytotoxic effects of Sal B on NPFs were evaluated by performing the Cell Counting Kit-8 assay.

The Transwell assay was conducted to assess cell migration. α-smooth muscle actin (α-SMA), TGF-β1 receptor (TβR)-I, TβR-II, Smad2/3 mRNA and protein expression levels and (p)-Smad2/3 phosphorylation levels were measured via reverse transcription-quantitative PCR and western blotting, respectively. Type III collagen and fibronectin levels were analyzed by ELISA. The results indicated that Sal B significantly downregulated TGF-β1-induced α-SMA, fibronectin and collagen III expression levels in NPFs. Similarly, Sal B significantly decreased TGF-β1-induced TβR-I, TβR-II, p-Smad2/3, MMP-2 and MMP-9 mRNA and protein expression levels in NPFs. Furthermore, Sal B significantly decreased TGF-β1-induced NPF migration. Therefore, the present study indicated that Sal B inhibited myofibroblast differentiation and ECM accumulation in nasal fibroblasts, suggesting that Sal B may inhibit nasal polyp formation via certain mechanisms.

E2F2 stimule l’expression de CCR4 et active les cellules de type fibroblaste synovial dans la polyarthrite rhumatoïde 

Aim of the study: E2F transcription factor 2 (E2F2) has increased expression in synovial tissues of rheumatoid arthritis (RA) and stimulates interleukin (IL)-1 α and IL-β production in cultured RA synovial fibroblast-like cells (RASF), which supports the importance of E2F2 in RA pathogenesis. This study investigated the effect and mechanism of E2F2 in RA.

Material and methods: Cultured RASF were transfected with anti-E2F2 siRNA, and the expression profile was analyzed with an inflammatory response and autoimmunity PCR array loaded with 84-relative genes to explore the pathogenic pathway of E2F2. Apoptosis, migration and tube-like structure formation in the RASF with transfection of anti-E2F2 siRNA or E2F2-expressing plasmids were examined using flow cytometry, transwell assays and Matrigel assays, respectively.

Results: Significantly decreased expression of chemokine receptor 4 (CCR4) was detected in RASF with inhibited E2F2 expression, and the CCR4 expression was increased in RASF with transfection of E2F2-expressing plasmids. Silencing E2F2 expression stimulated apoptosis, but retarded migration and tube-like structure formation in RASF. The opposite observation was obtained in RASF with E2F2 overexpression.

Conclusions: High E2F2 expression decreases apoptosis and increases migration and tube-like structure ability in RASF and might perform this role by up-regulating CCR4 expression, which ultimately contributes to the disease progression of RA synovial tissues.

Taux de sécrétion et composition protéique des vésicules extracellulaires libérées par les fibroblastes associés au cancer après irradiation 

Reciprocal communication between the malignant and non-malignant cellular elements in tumors is essential for cancer sustainability and plays an important role in the response of cancers to treatments. Some of this cellular crosstalk takes place via secretion of vesicles that are actively released into the extracellular space by most cell types in tumors. Recent studies have demonstrated radiation-induced changes in the secretion rate and composition of extracellular vesicles (EVs), with impact on radiation-related cellular communication.

However, little is known about the effects of different radiation regimens on the release of EVs by cells of the tumor microenvironment. In this study, we provide a comprehensive molecular characterization of EVs released by cultured primary lung tumor fibroblasts. We explore the quantitative and morphological changes triggered by ionizing radiation (IR), delivered as a single dose of 18 Gy or three consecutive daily medium-doses of 6 Gy. Cancer-associated fibroblasts (CAFs) secrete EVs with sizes ranging from 80 to 200 nm, expressing some of the classical exosome markers. Exposing CAFs to a single-high radiation dose (1 × 18 Gy) or fractionated medium-dose did not alter the release of CAF-EVs.

The protein composition of CAF-EVs was analyzed by LC-MS/MS proteomics and revealed that CAF-EVs are enriched with heat shock proteins, integrins, tetraspanins, proteinases, collagens, growth factors and an array of molecules involved in the regulation of cell migration and the immune system. Quantitative proteomic analyses revealed minor changes in the protein composition of CAF-EVs after radiation exposure. Taken together, this study presents original data on lung tumor CAF-EV composition and reveals that release and protein cargo of CAF-EVs are largely unaltered after exposing CAFs to IR.

Fibroblasts
MBS6507500-5x01mg 5x0.1mg
EUR 3395

Fibroblasts/Epithelial Cells Antibody (RPE)
abx413037-100tests 100 tests
EUR 811.2

Fibroblasts/Epithelial Cells Antibody (RPE)
abx413038-25tests 25 tests
EUR 427.2

Fibroblasts / Epithelial Cells Antibody (RPE)
GWB-F0EF95 25 TESTS Ask for price

Fibroblasts / Epithelial Cells Antibody (RPE)
GWB-2B065D 100 TESTS Ask for price

OASA02809-25UG - FIBROBLASTS/EPITHELIAL CELLS Antibody
OASA02809-25UG 25ug
EUR 149

OASA02808-200UG - FIBROBLASTS/EPITHELIAL CELLS Antibody
OASA02808-200UG 0.2mg
EUR 499

Rat Lung Fibroblasts
ABC-TC4158 1 vial Ask for price
Description: Rat Primary Lung Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Lung Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Lung Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Skin Fibroblasts
ABC-TC4217 1 vial Ask for price
Description: Rat skin fibroblasts, 2-week Wistar rat

Rat Vein Fibroblasts
ABC-TC4240 1 vial Ask for price
Description: Rat Primary Vein Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Vein Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Vein Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat liver fibroblasts
ABC-TC4153 1 vial Ask for price
Description: Rat liver fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Mouse liver fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Dog liver Fibroblasts
cAP-d0010 1Frozen Vial
EUR 396

Mouse Lung Fibroblasts
ABC-TC3931 1 vial Ask for price
Description: Mouse lung fibroblasts, 2-week ICR mouse

Rat Aortic Fibroblasts
ABC-TC4070 1 vial Ask for price
Description: Rat Primary Aortic Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Aortic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Aortic Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Artery Fibroblasts
ABC-TC4074 1 vial Ask for price
Description: Rat Primary Artery Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Dermal Fibroblasts
ABC-TC4119 1 vial Ask for price
Description: Rat Dermal Fibroblasts (RDF) are derived from the dermis of normal Sprague Dawley rats. They are cryopreserved at first passage and can be cultured and propagated at least 8 population doublings.

Rat Kidney Fibroblasts
ABC-TC4147 1 vial Ask for price
Description: Rat kidney fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Rat kidney fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Rat Spleen Fibroblasts
ABC-TC4222 1 vial Ask for price
Description: Rat Primary Spleen Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Spleen Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Spleen Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Uterus Fibroblasts
ABC-TC4237 1 vial Ask for price
Description: Rat uterus fibroblasts, 6-week Wistar rat

Human Skin Fibroblasts
HXXFB-00001 1*10^6/vial
EUR 1529

Human Liver Fibroblasts
ABC-TC3672 1 vial Ask for price
Description: Human Liver Fibroblasts are isolated from normal human liver tissue. Human Liver Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml.

Mouse Aorta Fibroblasts
ABC-TC3912 1 vial Ask for price
Description: Mouse aorta fibroblasts, 2-week ICR mouse

Rabbit Lung Fibroblasts
ABC-TC4064 1 vial Ask for price
Description: Rabbit Primary Lung Fibroblasts from Gentaur are isolated from lung tissue of New Zealand White Rabbits. Rabbit Primary Lung Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rabbit Primary Lung Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rat bladder fibroblasts
ABC-TC4080 1 vial Ask for price
Description: Rat bladder fibroblasts, 3-week Wistar rat

Rat Cardiac Fibroblasts
ABC-TC4105 1 vial Ask for price
Description: Rat Primary Cardiac Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Cardiac Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Cardiac Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Mammary Fibroblasts
ABC-TC4167 1 vial Ask for price
Description: Rat Primary Mammary Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Mammary Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Mammary Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Ovarian Fibroblasts
ABC-TC4187 1 vial Ask for price
Description: Rat Primary Ovarian Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Ovarian Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Ovarian Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Thyroid Fibroblasts
ABC-TC4232 1 vial Ask for price
Description: Rat Primary Thyroid Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Thyroid Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Thyroid Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Cardiac Fibroblasts
CP-R074 5×105 cells/vial
EUR 550
Description: Rat

Human Fibroblasts, Dermis
ABC-TC3622 1 vial Ask for price
Description: The HFIB-D cultures are produced on the base of human tissues. The HFIB-D are isolated according to known procedures. Then primary cultures are produced. After further culturing, the HFIB-D are frozen in a computer controlled freezing unit.

Human Tonsil Fibroblasts
ABC-TC3835 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Fibroblasts are among the most accessible normal mammalian cell types and still the most amenable to culture in vitro.HTF from Gentaur Research Laboratories are isolated from human tonsils. HTF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HTF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HTF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Monkey Liver Fibroblasts
ABC-TC3908 1 vial Ask for price
Description: Monkey Primary Liver Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Liver Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Liver Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rat Prostate Fibroblasts
ABC-TC4198 1 vial Ask for price
Description: Rat Primary Prostate Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Prostate Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Prostate Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Tracheal Fibroblasts
ABC-TC4234 1 vial Ask for price
Description: Rat Primary Tracheal Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Tracheal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Tracheal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Vascular Fibroblasts
ABC-TC4238 1 vial Ask for price
Description: Rat vascular fibroblasts, 2-week Wistar rat

Human Kidney Fibroblasts
ABC-TC5519 1 vial Ask for price
Description: Human Kidney Fibroblasts are isolated from the human adult healthy renal tissue. HKF-a are cryopreserved after passage one culture and delivered frozen. HKF-a are guaranteed to further expand for 15 population doublings under the conditions provided by in the technical sheet.As an excretory organ in the urinary system, the kidney greatly influences an individual’s well being by preserving homeostasis through regulating electrolytes, pH, and blood pressure among other control mechanisms. Comprising the majority of interstitial cells in the kidney, renal fibroblasts are responsible for the synthesis of collagen I of the extracellular matrix. Also, renal cortical fibroblasts synthesize erythropoietin, an important glycoprotein hormone that controls the production of red blood cells and renal medullary fibroblasts produce prostaglandins, a class of autocrine or paracrine hormones, which help maintain water and electrolyte homeostasis. Renal interstitial fibrosis, a disorder that may escalate to chronic kidney disease, is often correlated with excessive deposition of extracellular matrix by renal fibroblasts, the regulation of which process is under intensive research.

Human Kidney Fibroblasts
CSC-8240W One Frozen vial Ask for price

Human Dermal Fibroblasts
CSC-C0519Z One Frozen vial Ask for price

Human Spleen Fibroblasts
CSC-C4868L One Frozen vial Ask for price

Native Human Fibroblasts
MBS556459-INQUIRE INQUIRE Ask for price

Human Adrenal Fibroblasts
ABC-TC3478 1 vial Ask for price
Description: The adrenal gland plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones. Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.HAdF from Gentaur Research Laboratories are isolated from human adrenal tissue. HAdF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HAdF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HAdF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAdF are guaranteed to further expand for 15 population doublings under the conditions provided by Gentaur Research Laboratories.

Human Cardiac Fibroblasts
ABC-TC3533 1 vial Ask for price
Description: The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors.HCF from Gentaur Research Laboratories are isolated from the human heart. HCF-a are cryopreserved at secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCF-a are characterized by immunofluorescent method with antibody to fibronectin. HCF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-a are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Mammary Fibroblasts
ABC-TC3694 1 vial Ask for price
Description: Fibroblasts are the most common types of mammary stromal cells. Mammary stromal cells differentiate into fibroblasts, adipocytes or vascular structures in a hormone- and substatum-dependent manner, and may explain the dramatic changes in stromal composition during both normal mammary gland development and tumorigenesis. The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. The Mammary stromal fibroblasts express and produce cytokines such as SF witch plays roles in mammary cancer. HMF from Gentaur Research Laboratories are isolated from human mammary tissue. HMF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HMF are characterized by immunofluorescent method with antibodies to fibronectin, CD90 and vimentin. HMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMF are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

Human Ovarian Fibroblasts
ABC-TC3741 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Ovarian fibroblasts stimulate human ovarian cancer cell invasion and expression of 72-kDa gelatinase A (MMP-2).HOF from Gentaur Research Laboratories are isolated from human ovary tissue. HOF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HOF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HOF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Splenic Fibroblasts
ABC-TC3818 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. There is good evidence that fibroblasts in different parts of the body are intrinsically different and display different regulatory functions on surrounding cells. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. HSF from Gentaur Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Thyroid Fibroblasts
ABC-TC3832 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Because the thyroid is easily susceptible to inflammatory autoimmune diseases that can result in tissue damage, thyroid fibroblasts may play an important role in regulating thyroid function and reaction to disease. HThF from Gentaur Research Laboratories are isolated from human thyroid tissue. HThF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HThF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HThF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HThF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Uterine Fibroblasts
ABC-TC3863 1 vial Ask for price
Description: Derived from the mesoderm, connective tissue is involved in maintaining the structural integrity of the body. The predominant cells found in connective tissue are fibroblasts, which continuously secrete various components of the extracellular matrix. Depending on the site of origin and physiological status, fibroblasts may exhibit different morphological phenotypes and various functional properties.Gentaur offers a range of Human Fibroblasts produced at Gentaur's cell culture facility. The cells are isolated from normal human juvenile foreskin or adult skin from different locations. In addition, Gentaur offers Fibroblasts from adult lung tissue, aortic adventitial tissue, and villous placental tissue.Shortly after isolation, all Gentaur Human Fibroblasts are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Monkey Aortic Fibroblasts
ABC-TC3903 1 vial Ask for price
Description: Monkey Primary Aortic Fibroblasts from Gentaur are isolated from aortic tissue of Cynomolgus Monkey. Monkey Primary Aortic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Monkey Dermal Fibroblasts
ABC-TC3905 1 vial Ask for price
Description: Monkey Primary Dermal Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Dermal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Dermal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rabbit Aortic Fibroblasts
ABC-TC4055 1 vial Ask for price
Description: Rabbit Primary Aortic Fibroblasts from Gentaur are isolated from aortic tissue of New Zealand White Rabbits. Rabbit Primary Aortic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rabbit Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rat Embryonic Fibroblasts
ABC-TC4128 1 vial Ask for price
Description: Rat primary embryonic fibroblasts are isolated from day 13.5 embryos of Sprague-Dawley rats. Rat primary embryonic fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Rat Lymphatic Fibroblasts
ABC-TC4164 1 vial Ask for price
Description: Rat Primary Lymphatic Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Lymphatic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Lymphatic Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Pulmonary Fibroblasts
ABC-TC4204 1 vial Ask for price
Description: The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions.

Human Gastric Fibroblasts
ABC-TC5516 1 vial Ask for price
Description: Gastric fibroblasts (GF), which are located in the stomach, are mesenchymal cells derived from the embryonic mesoderm. Fibroblasts secrete a non-rigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. Studies in vitro and in vivo show that GF can regulate epithelial regeneration by influencing epithelial cell proliferation and differentiation. In addition to their role in wound healing, human GF (HGF) have been shown to be key contributors in the development and progression of gastric carcinoma. HGF are a useful model for examining the underlying mechanisms that cause gastric carcinoma and may provide novel insights for developing new cancer drug therapies.

Human Splenic Fibroblasts 
CSC-C9230J One Frozen vial Ask for price

Human Thyroid Fibroblasts 
CSC-7702W One Frozen vial Ask for price

Human Colonic Fibroblasts
CSC-8242W One Frozen vial Ask for price

Human Hepatic Fibroblasts
CSC-C1500 One Frozen vial Ask for price

Human Cardiac Fibroblasts
CSC-C1503 One Frozen vial Ask for price

Human Mammary Fibroblasts
CSC-C1527 One Frozen vial Ask for price

Mouse Cardiac Fibroblasts
CP-M074 5×105 cells/vial
EUR 580
Description: Mouse

Human Gingival Fibroblasts
ABC-TC3627 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells with many vital functions during development and in adult organisms. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Fibroblasts are among the most accessible normal mammalian cell types and still the most amenable to culture in vitro. Gingival fibroblasts are the major constituents of gingival tissue and play a key role in their health maintenance. Human gingival fibroblasts (HGnF) express a wide surface molecular panel including mainly CD9, CD26, CD55, CD59, CD63, CD71, CD86 CD95, CD99 and CD117. They were also demonstrated to express mRNAs for protease-activated receptor-1 (PAR-1) and PAR-3.HGnF from Gentaur Research Laboratories are isolated from human gingiva. HGnF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HGnF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HGnF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGnF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Prostate Fibroblasts
ABC-TC3765 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of the easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Cultured prostate fibroblasts synthesize FGF-like growth factor that stimulate their growth and is believed to be important in the development of benign prostatic hyperplasia.HPrF from Gentaur Research Laboratories are isolated from human prostate tissue. HPrF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPrF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HPrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPrF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Monkey Cardiac Fibroblasts
ABC-TC3904 1 vial Ask for price
Description: Monkey Primary Cardiac Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Cardiac Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Cardiac Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rat Intestinal Fibroblasts
ABC-TC4141 1 vial Ask for price
Description: Rat Primary Intestinal Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Intestinal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Intestinal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Rat Perineural Fibroblasts
ABC-TC4191 1 vial Ask for price
Description: Perineurial cells are of mesenchymal origin. They make up the perineurium, which has the important role of maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier.

Rabbit Cardiac Fibroblasts
ABC-TC5512 1 vial Ask for price
Description: Rabbit Primary Cardiac Fibroblasts from Gentaur are isolated from heart tissue of New Zealand White Rabbit. Rabbit Primary Cardiac Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen.

Porcine Aortic Fibroblasts
ABC-HP020X 1 vial Ask for price
Description: Porcine Aortic Fibroblasts are isolated from aorta of porcine. Porcine Aortic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials.

Human Prostate Fibroblasts 
CSC-7730W One Frozen vial Ask for price

Human Prostate Fibroblasts
ALHE28 1ml frozen Vial
EUR 620

Human Gingival Fibroblasts
cAP-0140 1Frozen Vial
EUR 693

Human pancreas fibroblasts
P0018025 One Frozen vial
EUR 580

Human Bronchial Fibroblasts
ABC-TC3529 1 vial Ask for price
Description: Human Bronchial Fibroblasts from Gentaur Research Laboratories are isolated from human bronchus tissue. HBF are cryopreserved at secondary culture (P1) and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBF are characterized by immunofluorescent method with antibody to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Lymphatic Fibroblasts
ABC-TC3688 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Besides, dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.HLF from Gentaur Research Laboratories are isolated from human lymph nodes. HLF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Pulmonary Fibroblasts
ABC-TC3776 1 vial Ask for price
Description: The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. HPF from Gentaur Research Laboratories are isolated from human lung tissue. HPF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPF are characterized by immunofluorescent method with antibody to fibronectin. HPF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Rabbit Vascular Fibroblasts
ABC-TC4066 1 vial Ask for price
Description: Rabbit vascular fibroblasts, 5-week rabbit

Human Intestine Fibroblasts
cAP-0206 1Frozen Vial
EUR 544.5

Human Esophageal Fibroblasts
ABC-TC3613 1 vial Ask for price
Description: Esophageal fibroblasts which line the lamina propria of the upper and lower esophagus are mesenchymal cells that originated from the embryonic mesoderm. They have been shown to play an important role in angiogenesis through TGF-β and VEGF signaling pathways in the highly angiogenic esophageal squamous cell carcinoma. Predominantly, fibroblasts secrete a nonrigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in the connective tissues and play major roles in wound healing. Because they are one of the simplest types of cells to grow in culture fibroblasts have been extensively used for a wide range of cellular and molecular studies. In addition, fibroblasts’ durability makes them amenable to a wide variety of manipulations ranging from gene transfection to microinjection.The HEF cells from Gentaur Research Laboratories are isolated from human esophageal tissue. HEF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEF are characterized by immunofluorescence with antibody specific to fibronectin. HEF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEF are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

Human Intestinal Fibroblasts
ABC-TC3655 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.HIF from Gentaur Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Monkey Lymphatic Fibroblasts
ABC-TC3910 1 vial Ask for price
Description: Monkey Primary Lymphatic Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Lymphatic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Lymphatic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Mouse Intestinal Fibroblasts
ABC-TC3929 1 vial Ask for price
Description: Mouse intestinal fibroblasts, 2-week C57B mouse

Human Pancreatic Fibroblasts
ABC-TC5470 1 vial Ask for price
Description: Human Primary Pancreatic Fibroblasts are isolated from normal human pancreatic tissue. Human Primary Pancreatic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml.

Human Pancreatic Fibroblasts
CSC-C4882L One Frozen vial Ask for price

Human Pancreatic Fibroblasts
cAP-0144 1Frozen Vial
EUR 693

Fibroblasts, Epithelial Cells
MBS609129-02mg 0.2mg
EUR 820

Fibroblasts, Epithelial Cells
MBS609129-5x02mg 5x0.2mg
EUR 3550

Human Gallbladder Fibroblasts
ABC-TC3625 1 vial Ask for price
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. Because they are one of the easiest types of cells to grow in culture, they have been extensively used for a wide range of cellular and molecular studies. Additionally, their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Human gallbladder fibroblasts (HGBF) are responsible for eicosanoid synthesis, specifically PGI2. Chronic stimulation of HGBF results in up-regulated synthesis and release of PGI2 which aids in formation of early acute cholecystitis. HGBF are very versatile cells and could be used in studies of many conditions, such as the early stages of acute cholecystitis.HGBF from Gentaur Research Laboratories are isolated from human gallbladder tissue. HGBF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HGBF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HGBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGBF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Pericardial Fibroblasts
ABC-TC3748 1 vial Ask for price
Description: Pericardial Fibroblasts are the principal cell type of the serous pericardium. They help to form the strong connective tissue that allows the pericardium to serve as a protective sac for the heart. Pericardial fibroblasts are exposed to a dynamic mechanical environment, being attached to the diaphragm, which influences the structure integrity of both healthy and healing tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen and there is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts have been extensively used for a wide range of cellular and molecular studies because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.HPcF from Gentaur Research Laboratories are isolated from the human heart. HPcF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPcF are characterized by immunofluorescent method with antibody to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPcF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Monkey Intestinal Fibroblasts
ABC-TC3906 1 vial Ask for price
Description: Monkey Primary Intestinal Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Intestinal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Intestinal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Rabbit Intestinal Fibroblasts
ABC-TC4060 1 vial Ask for price
Description: Rabbit Primary Intestinal Fibroblasts from Gentaur are isolated from Intestinal tissue of New Zealand White Rabbits. Rabbit Primary Intestinal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rabbit Primary Intestinal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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