La glycation par le glyoxal entraîne de profonds changements dans le comportement des fibroblastes dermiques
Introduction: Diabetes is a worldwide well being downside that’s related to extreme issues. Superior Glycation Finish merchandise (AGEs) akin to Nε-(carboxymethyl)lysine, which end result from power hyperglycemia, accumulate within the pores and skin of sufferers with diabetes. The impact of AGEs on fibroblast performance and their affect on wound therapeutic are nonetheless poorly understood.
Analysis design and strategies: To analyze this, we handled cultured human fibroblasts with 0.6 mM glyoxal to induce acute glycation. The conduct of fibroblasts was analyzed by time-lapse monolayer wounding therapeutic assay, seahorse know-how and atomic drive microscopy. Manufacturing of extracellular matrix was studied by transmission digital microscopy and western blot. Lipid metabolism was investigated by staining of lipid droplets (LDs) with BODIPY 493/503.
Outcomes: We discovered that the proliferative and migratory capacities of the cells had been vastly lowered by glycation, which might be defined by a rise in fibroblast tensile energy. Measurement of the mobile vitality steadiness didn’t point out that there was a change within the price of oxygen consumption of the fibroblasts. Evaluation of collagen I revealed that glyoxal didn’t affect kind I collagen secretion though it did disrupt collagen I maturation and it prevented its deposition within the extracellular matrix. We famous a pronounced enhance within the variety of LDs after glyoxal therapy. AMPK phosphorylation was lowered by glyoxal therapy but it surely was not accountable for the buildup of LDs.
Conclusion: Glyoxal promotes a change in fibroblast conduct in favor of lipogenic exercise that might be concerned in delaying wound therapeutic.
Fibroblasts/Epithelial Cells Antibody |
|||
abx413036-25ug | Abbexa | 25 ug | EUR 360 |
Fibroblasts/Epithelial Cells Antibody |
|||
GWB-Q01116 | GenWay Biotech | 0.2 mg | Ask for price |
Fibroblasts/Epithelial Cells Antibody |
|||
GWB-Q01117 | GenWay Biotech | 25 ug | Ask for price |
Fibroblasts/Epithelial Cells Antibody (PE) |
|||
abx413038-200l | Abbexa | 200 µl | EUR 250 |
Fibroblasts/Epithelial Cells Antibody (RPE) |
|||
abx413037-100tests | Abbexa | 100 tests | EUR 811.2 |
Fibroblasts/Epithelial Cells Antibody (RPE) |
|||
abx413038-25tests | Abbexa | 25 tests | EUR 427.2 |
Fibroblasts / Epithelial Cells Antibody (RPE) |
|||
GWB-2B065D | GenWay Biotech | 100 TESTS | Ask for price |
Fibroblasts / Epithelial Cells Antibody (RPE) |
|||
GWB-F0EF95 | GenWay Biotech | 25 TESTS | Ask for price |
Fibroblasts, Epithelial Cells |
|||
MBS609129-02mg | MyBiosource | 0.2mg | EUR 820 |
Fibroblasts, Epithelial Cells |
|||
MBS609129-5x02mg | MyBiosource | 5x0.2mg | EUR 3550 |
Fibroblasts, Epithelial Cells (AP) |
|||
MBS6247938-01mL | MyBiosource | 0.1(mL | EUR 1105 |
Fibroblasts, Epithelial Cells (AP) |
|||
MBS6247938-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
Fibroblasts, Epithelial Cells (HRP) |
|||
MBS6250880-01mL | MyBiosource | 0.1(mL | EUR 1105 |
Fibroblasts, Epithelial Cells (HRP) |
|||
MBS6250880-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
Fibroblasts, Epithelial Cells (APC) |
|||
MBS6268791-01mL | MyBiosource | 0.1mL | EUR 1105 |
Fibroblasts, Epithelial Cells (APC) |
|||
MBS6268791-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
OASA02809-25UG - FIBROBLASTS/EPITHELIAL CELLS Antibody |
|||
OASA02809-25UG | Aviva Systems Biology | 25ug | EUR 149 |
OASA02808-200UG - FIBROBLASTS/EPITHELIAL CELLS Antibody |
|||
OASA02808-200UG | Aviva Systems Biology | 0.2mg | EUR 499 |
Fibroblasts, Epithelial Cells (FITC) |
|||
MBS6249884-01mL | MyBiosource | 0.1(mL | EUR 1105 |
Fibroblasts, Epithelial Cells (FITC) |
|||
MBS6249884-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
Fibroblasts, Epithelial Cells (Biotin) |
|||
MBS6248958-01mL | MyBiosource | 0.1(mL | EUR 1105 |
Fibroblasts, Epithelial Cells (Biotin) |
|||
MBS6248958-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
Fibroblasts, Epithelial Cells (MaxLight 405) |
|||
MBS6252846-01mL | MyBiosource | 0.1(mL | EUR 1105 |
Fibroblasts, Epithelial Cells (MaxLight 405) |
|||
MBS6252846-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
Fibroblasts, Epithelial Cells (MaxLight 650) |
|||
MBS6255966-01mL | MyBiosource | 0.1(mL | EUR 1105 |
Fibroblasts, Epithelial Cells (MaxLight 650) |
|||
MBS6255966-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
Fibroblasts, Epithelial Cells (MaxLight 550) |
|||
MBS6254926-01mL | MyBiosource | 0.1(mL | EUR 1105 |
Fibroblasts, Epithelial Cells (MaxLight 550) |
|||
MBS6254926-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
Fibroblasts, Epithelial Cells (MaxLight 750) |
|||
MBS6257005-01mL | MyBiosource | 0.1mL | EUR 1105 |
Fibroblasts, Epithelial Cells (MaxLight 750) |
|||
MBS6257005-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
Fibroblasts, Epithelial Cells (MaxLight 490) |
|||
MBS6253886-01mL | MyBiosource | 0.1(mL | EUR 1105 |
Fibroblasts, Epithelial Cells (MaxLight 490) |
|||
MBS6253886-5x01mL | MyBiosource | 5x0.1mL | EUR 4815 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS |
|||
MBS214991-02mg | MyBiosource | 0.2mg | EUR 550 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS |
|||
MBS214991-5x02mg | MyBiosource | 5x0.2mg | EUR 2295 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS |
|||
MBS214992-0025mg | MyBiosource | 0.025mg | EUR 225 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS |
|||
MBS214992-5x0025mg | MyBiosource | 5x0.025mg | EUR 835 |
OASA02810-100T - Fibroblasts/Epithelial Cells Antibody - PE Conjugated |
|||
OASA02810-100T | Aviva Systems Biology | 100Tests | EUR 519 |
OASA02811-25T - Fibroblasts/Epithelial Cells Antibody - PE Conjugated |
|||
OASA02811-25T | Aviva Systems Biology | 25Tests | EUR 219 |
Mouse Anti Human Fibroblasts/Epithelial Cells Monoclonal Antibody,RPE |
|||
DMABT-49595MH | Creative Diagnostics | 25 TEST | EUR 382.2 |
Description: Mouse |
Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, PE |
|||
SM1214R | Origene Technologies GmbH | 100 Tests | Ask for price |
Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, PE |
|||
SM1214RT | Origene Technologies GmbH | 25 Tests | Ask for price |
Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, Purified |
|||
SM1214P | Origene Technologies GmbH | 200 µg | Ask for price |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE |
|||
MBS214993-100Tests | MyBiosource | 100Tests | EUR 575 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE |
|||
MBS214993-5x100Tests | MyBiosource | 5x100Tests | EUR 2410 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE |
|||
MBS214994-25Tests | MyBiosource | 25Tests | EUR 270 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE |
|||
MBS214994-5x25Tests | MyBiosource | 5x25Tests | EUR 1035 |
Cartilage Dissociation System 1 (Mesenchymal, Fibroblasts, Epithelial-like), Mouse and Rat |
|||
4-20231 | CHI Scientific | ea | EUR 161.87 |
Human Skin Cells (Fibroblasts) |
|||
ABC-TC3807 | AcceGen | 1 vial | Ask for price |
Description: Human fibroblasts are derived from cultured skin explants. These fibroblasts are from a single donor. |
Human Primary Pulmonary Fibrosis Bronchial Epithelial Cells |
|||
T5462 | ABM | 1 x 10^6 / 1.0 ml | Ask for price |
Human Skin Cells (Fibroblasts, Prenata) |
|||
ABC-TC3809 | AcceGen | 1 vial | Ask for price |
Description: Human fibroblasts are derived from cultured skin explants. Human fibroblasts are from a single donor. These cells enable researchers to study skin diseases such as dermatitis, wound healing, and other diseases that are expressed in fibroblast. In addition, they may be used to study the development of skin and production of inducible pluripotent stem cells. Development period: Prenata |
Normal Lung Fibroblasts 1,000,000 cells per vial. |
|||
C07ADDON | VITRO BIOPHARMAVITRO BIOPHARMA | 1,000,000 cells | EUR 682.5 |
Normal Colon Fibroblasts 1,000,000 cells per vial. |
|||
C05DON | VITRO BIOPHARMAVITRO BIOPHARMA | 1,000,000 cells | EUR 682.5 |
Human Skin Cells (Fibroblasts, Postnatal) |
|||
ABC-TC3808 | AcceGen | 1 vial | Ask for price |
Description: Human fibroblasts are derived from cultured skin explants. Human fibroblasts are from a single donor. These cells enable researchers to study skin diseases such as dermatitis, wound healing, and other diseases that are expressed in fibroblast. In addition, they may be used to study the development of skin and production of inducible pluripotent stem cells. Development period: Postnatal |
Human Primary ∆F508/∆1152H Cystic Fibrosis Bronchial Epithelial Cells |
|||
T5425 | ABM | 5 x 10^5 / 1.0 ml | Ask for price |
DHBE-CF (Diseased Human Bronchial Epithelial Cells - Cystic Fibrosis) |
|||
LO196979 | Westburg | each | EUR 2914.41 |
Human Primary DF508/DF508 Cystic Fibrosis Bronchial Epithelial Cells |
|||
T5423 | ABM | 5 x 10^5 / 1.0 ml | Ask for price |
Human Primary DF508/N1303K Cystic Fibrosis Bronchial Epithelial Cells |
|||
T5424 | ABM | 5 x 10^5 / 1.0 ml | Ask for price |
Human Primary DF508/S549N Cystic Fibrosis Bronchial Epithelial Cells |
|||
T5427 | ABM | 1 x 10^6 / 1.0 ml | Ask for price |
D-SAEC (Diseased Small Airway Epithelial Cells – Cystic Fibrosis), cryo |
|||
LOCC-2933 | Westburg | each | EUR 2181.55 |
Immortalized Human Pulmonary Fibroblasts Cells |
|||
CSC-I9127L | Creative Bioarray | One Frozen vial | Ask for price |
Immortalized Human Pulmonary Fibroblasts Cells |
|||
T0490 | ABM | 1x106 cells / 1.0 ml | EUR 2750 |
Mouse Fibroblasts cryo amp, 4 mil cells |
|||
LOM-FB-481 | Westburg | each | EUR 895.66 |
Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1) |
|||
T0681 | ABM | 1x106 cells / 1.0 ml | EUR 686.4 |
Rat Lens Epithelial Cells |
|||
ABC-TC4150 | AcceGen | 1 vial | Ask for price |
Description: The cells that make up the mammalian lens consist of two types: lens fiber cells, which form the bulk of the lens, and a monolayer of epithelial cells cover the anterior surface of the fibers. |
Rat Liver Epithelial Cells |
|||
ABC-TC4152 | AcceGen | 1 vial | Ask for price |
Description: Rat Liver Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Liver Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Liver Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Liver Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Human Primary DF508/621+1G>T Cystic Fibrosis Bronchial Epithelial Cells |
|||
T5426 | ABM | 5 x 10^5 / 1.0 ml | Ask for price |
Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells-SV40 |
|||
CSC-I2271Z | Creative Bioarray | One Frozen vial | Ask for price |
Human Lens Epithelial Cells |
|||
ABC-TC3669 | AcceGen | 1 vial | Ask for price |
Description: Human Lens Epithelial Cells from Gentaur Research Laboratories are isolated from the human lens. HLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLEpiC are guaranteed to further culture in the conditions provided by Gentaur Research Laboratories. |
Rat Cornea Epithelial Cells |
|||
ABC-TC4115 | AcceGen | 1 vial | Ask for price |
Description: Rat cornea epithelial cells, 3-week Wistar rat |
Rat Dermal Epithelial Cells |
|||
ABC-TC4118 | AcceGen | 1 vial | Ask for price |
Description: Rat Dermal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Dermal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Dermal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Dermal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Kidney Epithelial Cells |
|||
ABC-TC4146 | AcceGen | 1 vial | Ask for price |
Description: Rat Kidney Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Kidney Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Kidney Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Kidney Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Spleen Epithelial Cells |
|||
ABC-TC4221 | AcceGen | 1 vial | Ask for price |
Description: Rat Spleen Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Spleen Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Spleen Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Spleen Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Human Oral Epithelial Cells |
|||
ABC-TC4365 | AcceGen | 1 vial | Ask for price |
Description: Cells are only guaranteed with purchase of Gentaur Media, Extra Cellular Matrix, Trypsin EDTA, 1X PBS, and freezing media for the appropriate cell culture, for 30 days from the date of shipment. |
Human Lens Epithelial Cells |
|||
CSC-7764W | Creative Bioarray | One Frozen vial | Ask for price |
Human Dermal Fibroblasts-fetal Feeder Cells |
|||
T2002 | ABM | 10^6 cells/1.0 ml | EUR 200 |
Human Nasal Epithelial Cells |
|||
ABC-TC3719 | AcceGen | 1 vial | Ask for price |
Description: The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane). Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) . Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Human Renal Epithelial Cells |
|||
ABC-TC3782 | AcceGen | 1 vial | Ask for price |
Description: Human Renal Epithelial Cells(Human kidney epithelial cells) are derived from whole kidneys that have been dissociated into single cells and purified in culture. Human kidney epithelial cells are from a single donor. These are highly purified epithelial cells as determined by specific epithelial cell markers. Human kidney epithelial cells enable researchers to study the development and biochemical function of the kidneys. In addition, they are an ideal cell for studying absorption and secretion that occurs in the kidneys. Development period: Prenatal |
Rat Colonic Epithelial Cells |
|||
ABC-TC4113 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Colonic Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Colonic Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Colonic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Colonic Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Corneal Epithelial Cells |
|||
ABC-TC4116 | AcceGen | 1 vial | Ask for price |
Description: Rat Corneal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Corneal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Corneal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Corneal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Mammary Epithelial Cells |
|||
ABC-TC4166 | AcceGen | 1 vial | Ask for price |
Description: Rat Mammary Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Mammary Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Mammary Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Mammary Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Ovarian Epithelial Cells |
|||
ABC-TC4186 | AcceGen | 1 vial | Ask for price |
Description: Rat Ovarian Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Ovarian Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Ovarian Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Ovarian Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Stomach Epithelial Cells |
|||
ABC-TC4228 | AcceGen | 1 vial | Ask for price |
Description: Rat Stomach Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Stomach Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Stomach Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Stomach Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Thyroid Epithelial Cells |
|||
ABC-TC4231 | AcceGen | 1 vial | Ask for price |
Description: Rat Thyroid Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Thyroid Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Thyroid Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Thyroid Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Bladder Epithelial Cells |
|||
ABC-TC5507 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Bladder Epithelial Cells from Gentaur are isolated from bladder tissue of 6-8 week old laboratory Sprague–Dawley rat. Rat Primary Bladder Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5x106 cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody. |
Human Renal Epithelial Cells |
|||
ALHE35 | Alphabioregen | 1ml frozen Vial | EUR 620 |
Human Nasal Epithelial Cells |
|||
CSC-C1511 | Creative Bioarray | One Frozen vial | Ask for price |
Human Tonsil Epithelial Cells |
|||
ABC-TC3834 | AcceGen | 1 vial | Ask for price |
Description: Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of actively differentiating epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium. The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues. In Situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial culture in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ. HTonEpiC from Gentaur Research Laboratories are isolated from human normal tonsil tissue. HTonEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HTonEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HTonEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTonEpiC are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories. |
Porcine Lens Epithelial Cells |
|||
ABC-TC4033 | AcceGen | 1 vial | Ask for price |
Description: The cells that make up the mammalian lens consist of two types: lens fiber cells, which form the bulk of the lens, and a monolayer of epithelial cells that cover the anterior surface of the fibers. |
Rat Prostate Epithelial Cells |
|||
ABC-TC4197 | AcceGen | 1 vial | Ask for price |
Description: Rat Prostate Epithelial Cells from Gentaur are isolated from tissue of adult laboratory Sprague–Dawley Rats. Rat Prostate Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Prostate Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Prostate Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Tracheal Epithelial Cells |
|||
ABC-TC4233 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Tracheal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Tracheal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Tracheal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Tracheal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Human Thymic Epithelial Cells |
|||
ABC-TC5517 | AcceGen | 1 vial | Ask for price |
Description: Thymic epithelial cells (TyEpiC) are vital regulators of thymocyte development and T lymphocyte (T-cell) tolerance. The thymus contains two types of TyEpiC, cortical and medullary thymic epithelial cells, which regulate the positive selection of thymocytes and the negative selection of autoreactive T-cells. The inability of TyEpiC to remove autoreactive T-cells in the thymus can affect self-tolerance and contribute to the development of autoimmune diseases such as myasthenia gravis, type 1 diabetes, rheumatoid arthritis, and multiple sclerosis [3]. Studies have shown that changes in the expression of tumor necrosis factor receptor family members and transcription factors Foxn1 and autoimmune regulator (Aire) can alter the thymic microenvironment and self-tolerance. Human TyEpiC are an excellent in vitro model to study the mechanisms that modulate TyEpiC functionality and to develop targeted treatments for autoimmune disorders. |
Renal Cancer Associated Fibroblasts, 1,000,000 Cells, cryopreserved |
|||
CAF04 | VITRO BIOPHARMAVITRO BIOPHARMA | 1,000,000 cells | EUR 1010.1 |
Human Stomach Epithelial Cells |
|||
ABC-HP021X | AcceGen | 1 vial | Ask for price |
Description: Human Stomach Epithelial Cells are isolated from normal human stomach tissue. Cells are cryopreserved at passage 3 and delivered frozen. |
Human Colonic Epithelial Cells |
|||
ABC-TC3570 | AcceGen | 1 vial | Ask for price |
Description: The colorectum is a major organ for both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host’s internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component of the mucosal immune system. Human colon epithelial cells can process and present antigens to T cells in vitro, and can be stimulated to express HLA class II and intercellular adhesion molecules in vivo. They also respond to a broad array of cytokines with altered gene expression and growth characteristics. In addition, normal colonic epithelial cells were found to express the integrin α3, α5, α6, β1 and β4 chains. HCoEpiC from Gentaur Research Laboratories are isolated from human colonic tissue. HCoEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCoEpiC are characterized by immunofluorescent method with antibodies to CK18 and CK19. HCoEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoEpiC are guaranteed to further culture for 15 population doublings in the conditions provided by Gentaur Research Laboratories. |
Human Corneal Epithelial Cells |
|||
ABC-TC3577 | AcceGen | 1 vial | Ask for price |
Description: The cornea is a unique tissue for two reasons: the transparency function and the synthesis of most of its proteins due to the cornea being an avascular tissue. The cornea consists of three distinct cell layers, the outer epithelium, the inner endothelium and the central stroma (the keratocytes). The corneal epithelium plays a role in the innate immune response by sensing the presence of pathogens and providing signals that activate the corneal defense system. It also highly expresses aldehyde dehydrogenase to protect against UV- and 4-hydroxynonenal-induced cellular damage. Corneal epithelial basal cell proliferation is controlled by a host of cytokines, e.g., epidermal growth factor, that activate their cognate receptors in the deeper layers of the epithelium. Instead of testing products in the eyes of animals, cultured corneal epithelial cells could be used for toxicology tests in in vitro models and for studying the molecular mechanisms involved in regulating human corneal epithelial cell differentiation.HCEpiC from Gentaur Research Laboratories are isolated from human cornea. HCEpiC are cryopreserved from passage one culture. Each vial contains 5×10^5 cells in 1 ml volume. HCEpiC are characterized by their cobble stone morphology in serum-free culture and immunofluorescent method with antibody to CK-18 and -19. HCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCEpiC are guaranteed to further expand for 10 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Urothel Epithelial Cells |
|||
ABC-TC3860 | AcceGen | 1 vial | Ask for price |
Description: The HUEPC cultures are produced on the base of human tissues. The HUEPC are isolated according to known procedures. Then primary cultures are produced. After further culturing, the HUEPC are frozen in a computer controlled freezing unit. |
Rat Bronchial Epithelial Cells |
|||
ABC-TC4284 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Bronchial Epithelial Cells from Gentaur are isolated from bronchial tissue of 6-8 week old laboratory Sprague–Dawley rat. Rat Primary Bronchial Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur’ Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5×10^6 cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody. |
Human Bladder Epithelial Cells |
|||
ABC-TC4367 | AcceGen | 1 vial | Ask for price |
Description: Human Primary Bladder Epithelial Cells from Gentaur are isolated from normal human bladder tissue. Human Primary Bladder Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaurandrsquo; Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody. |
Human Stomach Epithelial Cells |
|||
CSC-C9381W | Creative Bioarray | One Frozen vial | Ask for price |
Human Bladder Epithelial Cells |
|||
ALHE36 | Alphabioregen | 1ml frozen Vial | EUR 620 |
Human Colonic Epithelial Cells |
|||
CSC-C1488 | Creative Bioarray | One Frozen vial | Ask for price |
Human Corneal Epithelial Cells |
|||
cAP-0211 | Angio Proteomie | 1Frozen Vial | EUR 594 |
Human Amniotic Epithelial Cells |
|||
ABC-TC3486 | AcceGen | 1 vial | Ask for price |
Description: Human amniotic membrane is composed of an epithelial cell layer, a basement membrane and an avascular matrix. The amniotic epithelial cells (AEC) are formed from epiblasts on the 8th day after fertilization. A probable result of their embryonic origin, AEC lack major histocompatibility complex antigens and have been used for allotranplantation to treat patients with lysosomal diseases. Studies have shown that AEC have multiple functions such as synthesis and release of acetylcholine and catecholamine as well as expressing mRNA coding for DA receptors and DA transporters. They express neuronal and glial cell marks, produce basic fibroblast growth factors and hepatocyte growth factors and transform growth factor-beta. Human AEC has been suggested as an appropriate human cell model for studying DA release and uptake processes, receptor signal transduction and exploring newly developed drugs acting at these receptors.HAEpiC from Gentaur Research Laboratories are isolated from human amniotic membranes. HAEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HAEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories. |
Human Tracheal Epithelial Cells |
|||
ABC-TC3839 | AcceGen | 1 vial | Ask for price |
Description: The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelia Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi,and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Human Prostate Epithelial Cells |
|||
ABC-TC3995 | AcceGen | 1 vial | Ask for price |
Description: Human Prostate Epithelial Cells (HPrEC), when grown in LIPro Medium, provides an ideal serum-free culture model, without retinoic acid, for the study of prostate cells. Common uses for HPrEC are studies of hormonal regulation of the prostate, regulation and control of the secretory function of prostate cells and as a control for the study of prostate cancer. HPrEC are cryopreserved as secondary cells to ensure the highest viability and plating efficiency. Our HPrEC are quality tested in LIPro Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented media. Cell Features:HPrEC are cryopreserved as secondary cells, e.g. cells are isolated fromhuman prostate tissue and expanded twice in culture vessels before beingharvested for cryopreservation.HPrEC proliferate in a serum-free, retinoic acid-free medium withoutphenol red or antimicrobials when cultured in ProstaLifeTM medium.HPrEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality. |
Rat Esophageal Epithelial Cells |
|||
ABC-TC4133 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Esophageal Epithelial Cells from Gentaur are isolated from tissue of adult laboratory Sprague–Dawley Rats. Rat Primary Esophageal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Esophageal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Esophageal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Intestinal Epithelial Cells |
|||
ABC-TC4140 | AcceGen | 1 vial | Ask for price |
Description: Rat Intestinal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Intestinal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Intestinal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Intestinal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Pancreatic Epithelial Cells |
|||
ABC-TC4190 | AcceGen | 1 vial | Ask for price |
Description: Rat Pancreatic Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Pancreatic Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Pancreatic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Pancreatic Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Human Cervical Epithelial Cells |
|||
ABC-TC5466 | AcceGen | 1 vial | Ask for price |
Description: Cervical Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreserved to ensure the highest purity, viability and plating efficiency. HCxEC are quality tested in Epithelial Cell Medium to ensure optimal morphology and growth over a period of at least 5 population doublings. Cervical Epithelial Cells are not exposed to antimicrobials or phenol red when cultured in Epithelial Cell Medium. Gentaur offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation. |
Human Prostate Epithelial Cells |
|||
ALHE25 | Alphabioregen | 1ml frozen Vial | EUR 620 |
Human Alveolar Epithelial Cells |
|||
CSC-7845W | Creative Bioarray | One Frozen vial | Ask for price |
Human Alveolar Epithelial Cells |
|||
cAP-0208 | Angio Proteomie | 1Frozen Vial | EUR 643.5 |
Prostate Cancer Associated Fibroblasts, 1,000,000 Cells, cryopreserved |
|||
CAF10 | VITRO BIOPHARMAVITRO BIOPHARMA | 1,000,000 cells | EUR 1052.22 |
Gastric Cancer Associated Fibroblasts, 1,000,000 Cells, cryopreserved |
|||
CAF11 | VITRO BIOPHARMAVITRO BIOPHARMA | 1,000,000 cells | EUR 1052.22 |
Human Bronchial Epithelial Cells |
|||
ABC-TC3528 | AcceGen | 1 vial | Ask for price |
Description: The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelial Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Human Placental Epithelial Cells |
|||
ABC-TC3753 | AcceGen | 1 vial | Ask for price |
Description: The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelial Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Porcine Corneal Epithelial Cells |
|||
ABC-TC4029 | AcceGen | 1 vial | Ask for price |
Description: The cornea is a unique tissue for two reasons: the transparency function and the synthesis of most of its proteins due to the cornea being an avascular tissue. |
Canine Alveolar Epithelial Cells |
|||
ABC-TC5511 | AcceGen | 1 vial | Ask for price |
Description: Canine Primary Alveolar Epithelial Cells are isolated from alveolar tissue of beagle dog. Canine Primary Alveolar Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Culture Complete Growth Medium for 3-5 days. Prior to shipping, cells at passage 2 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. |
Human Laryngeal Epithelial Cells |
|||
ABC-TC5518 | AcceGen | 1 vial | Ask for price |
Description: Laryngeal Epithelial Cells are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. |
Human Bronchial Epithelial Cells |
|||
ALHE47 | Alphabioregen | 1ml frozen Vial | EUR 620 |
Human Bronchial Epithelial Cells |
|||
CSC-C4388X | Creative Bioarray | One Frozen vial | Ask for price |
Retinal Pigment Epithelial Cells |
|||
MBS556451-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Retinal Pigment Epithelial Cells |
|||
RPE001 | Neuromics | 500,000 Cells | EUR 1230.12 |
Human Esophageal Epithelial Cells |
|||
ABC-TC3612 | AcceGen | 1 vial | Ask for price |
Description: The human esophagus is lined by a non-keratinizing, moist stratified squamous epithelium whose apical cell membranes and intercellular junctional complexes combine to produce an effective permeability barrier against the influx of luminal content. In particular, the barrier created by these structures limits exposure of the surface cells' basolateral cell membranes and entire membrane of cells of the deeper layers to the wide swings in osmolality occurring regularly within the esophageal lumen. Histologically, the esophageal epithelium consists of two zones, the basal and differentiated zones. Cellular proliferation is limited to the basal zone, and cells are thought to migrate from this area towards the esophageal lumen. Migration is associated with the initiation of differentiation and the sequential expression of differentiation markers. The availability of human esophageal epithelial cell culture provides an excellent in vitro model in the study of the physiology of esophageal epithelium and the mechanisms of the esophageal carcinogenesis.HEEC from Gentaur Research Laboratories are isolated from the human esophagus. HEEC are cryopreserved on passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEEC are characterized by immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. HEEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEEC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories. |
Human Pancreatic Epithelial Cells |
|||
ABC-TC3744 | AcceGen | 1 vial | Ask for price |
Description: Human Pancreatic Epithelial Cell were initiated by elutriation of dispase dissociated normal pancreatic tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask. |
Human Intestinal Epithelial Cells |
|||
ABC-TC3879 | AcceGen | 1 vial | Ask for price |
Description: Cryopreserved InEpC are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures. In combination with human intestinal myofibroblasts (InMyoFib). |
Human Esophageal Epithelial Cells |
|||
CSC-7783W | Creative Bioarray | One Frozen vial | Ask for price |
Human Pancreatic Epithelial Cells |
|||
CSC-8246W | Creative Bioarray | One Frozen vial | Ask for price |
Human Endometrial Epithelial Cells |
|||
ABC-TC4601 | AcceGen | 1 vial | Ask for price |
Description: Proliferation and Morphology: Normal morphology for 5 population doublings, minimum 70% viability when thawed from cryopreservation. Normal Human Endometrial (Uterine) Epithelial Cells provide an ideal serum-free culture model for many areas of research. These cells may be used to study cellular physiology of the reproductive tract, the cellular response to infectious agents, and other areas of research, Endometrial Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency. |
Bovine Endometrium Epithelial Cells |
|||
ABC-TC3131 | AcceGen | 1 vial | Ask for price |
Description: Bovine Endometrial Epithelial Cells (BEnEpC) are isolated from epithelium of the bovine endometrium. The cells are cryopreserved at second passage. BEnEpC can be cultured and propagated for at least 10 population doublings. |
Human Conjunctival Epithelial Cells |
|||
ABC-TC3573 | AcceGen | 1 vial | Ask for price |
Description: The conjunctival epithelium is a stratified, non-keratinized epithelium. It can be distinguished by the corneal epithelium by the expression of different cytokeratins, mucins and by the presence of glycocalix. Decrease or loss of mucin/glycocalix production generates squamous metaplasia, which may lead to dry eye and ocular surface diseases. Human conjunctival epithelial cells represent potential target cells for mast cell mediators. They respond to histamine by generating inositol phosphates, mobilizing intracellular calcium, and then secreting proinflammatory cytokines like interleukin-6 and interleukin-8.HConEC from Gentaur Research Laboratories are isolated from human conjunctiva. HConEC are cryopreserved from passage one culture. Each vial contains 5×10^5 cells in 1 ml volume. HConEC are characterized by their cobble stone morphology in serum-free culture and immunofluorescent method with antibody to CK-18 and -19. HConEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HConEC are guaranteed to further expand for 10 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Conjunctival Epithelial Cells |
|||
CSC-7802W | Creative Bioarray | One Frozen vial | Ask for price |
Human Nasopharyngeal Epithelial Cells |
|||
cAP-0222 | Angio Proteomie | 1Frozen Vial | EUR 4455 |
Epithelial Dissociation System 11 (Epithelial Cells), Mouse and Rat |
|||
4-20261 | CHI Scientific | ea | EUR 144.82 |
Rat Primary Lung Epithelial Cells |
|||
T4800 | ABM | 5x10^5 cells / 1.0 ml | EUR 625 |
Rat Renal Tubular Epithelial Cells |
|||
ABC-TC4210 | AcceGen | 1 vial | Ask for price |
Description: Renal tubular epithelial cells (RTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. |
Human Renal Mixed Epithelial Cells |
|||
ABC-TC4245 | AcceGen | 1 vial | Ask for price |
Description: Normal Human Renal Epithelial Cells, when grown in LIRen Medium, provide an ideal low-serum culture model for the study of renal function, metabolism, nephrotoxicity or cancer research. Renal Mixed Epithelial Cells are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our Renal Epithelial Cells are quality tested in LIRen Medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium. Cell Features:Mixed Renal Epithelial, Renal Cortical Epithelial, and Renal Medullary Epithelial are cryopreserved as primary cells isolated from human kidney tissue and expanded in culture vessels once before cryopreservation.Renal Proximal Tubule Epithelial are cryopreserved as secondary cells isolated from human kidney tissue and expanded in culture vessels twice before cryopreservation.All Renal Epithelial Cell types can be grown in a 0.5% serum medium without phenol red or antimicrobials when cultured in LIRen Medium.All Renal Epithelial Cell types are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality. |
Rat Primary Renal Epithelial Cells |
|||
T4936 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Human Iris Pigment Epithelial Cells |
|||
ABC-TC3658 | AcceGen | 1 vial | Ask for price |
Description: The iris is a pigmented disk with a variable aperture which forms the pupil of the eye. It is covered by squamous epithelium on anterior surface and 2 layers of pigmented epithelium on posterior. The iris pigment epithelial cells (IPE) share functional properties with retinal pigment epithelial cells (RPE) such as phagocytosis, degradation of rod outer segments and synthesis of trophic factors. In recent years, IPE have been transplanted into the subretinal space of eye to treat RPE defects. The grafted IPE were seen to survive for months. There was a remodeling of cellular layers in the subretinal space over time where grafted IPE joined the native RPE. The cellular response that developed exhibited macrophages and were similar to that observed following RPE transplantation. In vitro study also show that IPE expressing CD86, directly suppresses T cell activation via binding to cytotoxic T lymphocyte-associated antigen 4.HIPEpiC from Gentaur Research Laboratories are isolated from the human iris. HIPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HIPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, vimentin and fibronectin. HIPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIPEpiC are guaranteed to further expand for two passages (around 10 population doublings) in the conditions provided by Gentaur Research Laboratories. |
Human Mammary Epithelial Cells, Male |
|||
ABC-TC3693 | AcceGen | 1 vial | Ask for price |
Description: Human Mammary Epithelial Cells, when grown in LIMam Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.Cell Features:HMEC are cryopreserved as tertiary cells, e.g. cells are isolated from human mammary tissue and expanded three times in culture vessels before being harvested for cryopreservation.HMEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality. |
Human Small Airway Epithelial Cells |
|||
ABC-TC3811 | AcceGen | 1 vial | Ask for price |
Description: The small airways are located at the interface between alveoli and conducting airways. Airway epithelial cells forming a continuous lining to the airways play a unique role as a protective physical and functional barrier to external deleterious agents. These cells function in the regulation of immune responses by contributing to host defense through chemokine production, adhesion molecule expression, and possibly antigen presentation via HLA-DR expression. They also produce liquids contributing to pulmonary fluid balance. Many airway diseases, such as asthma, bronchiolitis, chronic obstructive pulmonary disease, and cystic fibrosis, involve damage to the airway surface epithelium. The human small airway epithelial cell culture may identify new therapeutic options in preventing amplified airway ailment and remodeling.HSAEpiC from Gentaur Research Laboratories are isolated from human lung tissue. HSAEpiC are cryopreserved at either primary culture or passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HSAEpiC are characterized by immunofluorescent method with antibodies CK-18, -19, and vimentin. HSAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSAEpiC are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories. |
Human Small Airway Epithelial Cells |
|||
CSC-C9229J | Creative Bioarray | One Frozen vial | Ask for price |
Human Small Airway Epithelial Cells |
|||
ALHE50 | Alphabioregen | 1ml frozen Vial | EUR 620 |
Human Mammary Basal Epithelial Cells |
|||
ABC-TC3690 | AcceGen | 1 vial | Ask for price |
Description: human mammary basal epithelial cells are isolated from mammary tissue of healthy non-diabetic donors between 18 and 60 years old who have undergone elective surgery. The cells are isolated by digestion of the tissue to generate organoid structures. |
Human Mammary Epithelial Cells, Adult |
|||
ABC-TC3691 | AcceGen | 1 vial | Ask for price |
Description: The mammary gland consists of pseudostratified epithelia, with myoepithelila cells resting upon a basement membrane and apical glandular epithelial cells facing the lumen of the ducts and alveoli. Mammary epithelial cells undergo changes in growth, invasion, and differentiation throughout much of adulthood, and most strikingly during pregnancy, lactation, and involution. They proliferate and invade the surrounding extracellular matrix during the fetal and postnatal development of the gland, and then more vigorously at puberty as the branches of the mammary epithelial tree are formed.HMEpiC from Gentaur Research Laboratories are isolated from normal human breast tissue. HMEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HMEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HMEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMEpiC are guaranteed to further expand for 15 population doublings in the conditions provided by Gentaur Research Laboratories |
GFP-Human Corneal Epithelial Cells |
|||
cAP-0211GFP | Angio Proteomie | 1Frozen Vial | EUR 841.5 |
Human Primary Renal Epithelial Cells |
|||
T4090 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Rat Primary Bladder Epithelial Cells |
|||
T4940 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Human Primary Nasal Epithelial Cells |
|||
T4014 | ABM | 5x10^5 cells / 1.0 ml | EUR 1375 |
Human Choroid Plexus Epithelial Cells |
|||
ABC-TC3568 | AcceGen | 1 vial | Ask for price |
Description: The choroid plexus epithelial cells (CPEpiC) are the structural basis, form a selective barrier between the nervous system and the blood, and secrete cerebrospinal fluid into the ventricles of the brain. CPEpiC express inward-rectifying anion channels which are thought to have an important role in the secretion of cerebrospinal fluid. CPEpiC synthesize and secrete many growth factors and other peptides that are of trophic benefit following injury to regions of the cerebroventricular system. For example, several growth factors are upregulated in choroid plexus after ischemic and traumatic insults to the central nervous system. The presence of numerous types of growth factor receptors in choroid plexus allows growth factor mediation of recovery processes by autocrine and paracrine mechanisms. CPEpiC culture has been widely used to evaluate their morphological and physiological properties, and its capacity to serve as an in vitro model to access the cellular mechanisms of organic cation transport across the cerebrospinal fluid-blood barrier.HCPEpiC from Gentaur Research Laboratories are isolated from human choroid plexus. HCPEpiC are cryopreserved after purification and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HCPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCPEpiC are guaranteed to further expand for 15 population doublings in the conditions specified by Gentaur Research Laboratories. |
Human Mammary Epithelial Cells, Female |
|||
ABC-TC3692 | AcceGen | 1 vial | Ask for price |
Description: Human Mammary Epithelial Cells, when grown in LIMam Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HMEC include the stages of breast cancer development, three dimensional culture and carcinogen screening.Cell Features:HMEC are cryopreserved as tertiary cells, e.g. cells are isolated from human mammary tissue and expanded three times in culture vessels before being harvested for cryopreservation.HMEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality. |
Human Renal Cortical Epithelial Cells |
|||
ABC-TC4242 | AcceGen | 1 vial | Ask for price |
Description: Renal epithelial cells (REpiC) play a crucial role in renal function. Similar to most other epithelial cells, renal epithelial cells display a polarized morphology which is essential for their function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. Human REpiC are capable of internalizing bacteria. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. REpiC can produce inflammatory mediators such as cytokines and chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. REpiC express IL-2R alpha and MHC class II antigens during inflammation, indicating their capacity to participate in the pathogenesis of immune renal injury. To be able to study the relationship between renal epithelial cells and a variety of renal diseases, the HREpiC culture provides a useful in vitro model.HRCEpiC from Gentaur Research Laboratories are isolated from human kidneys. HRCEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HRCEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HRCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRCEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories. |
Human Fallopian Tube Epithelial Cells |
|||
ABC-TC5521 | AcceGen | 1 vial | Ask for price |
Description: Fallopian Tube Epithelial Cells have been isolated, plated and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest viability and plating efficiency. Fallopian Tube Cells are quality tested in our Medium to ensure optimal morphology and growth over a period of at least 5 population doublings. |
Human Renal Cortical Epithelial Cells |
|||
cAP-0139 | Angio Proteomie | 1Frozen Vial | EUR 693 |
Human Choroid Plexus Epithelial Cells. |
|||
cAP-0213 | Angio Proteomie | 1Frozen Vial | EUR 445.5 |
Rat Primary Tracheal Epithelial Cells |
|||
T4804 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Rat Primary Cervical Epithelial Cells |
|||
T4952 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Rat Primary Prostate Epithelial Cells |
|||
T4956 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Human Primary Thymic Epithelial Cells |
|||
T4980 | ABM | 5x10^5 cells / 1.0 ml | EUR 975 |
Human Large Intestine Epithelial Cells |
|||
ABC-TC3667 | AcceGen | 1 vial | Ask for price |
Description: This cell line is available in limited quantities. Please contact us for more information. |
Human Mammary Luminal Epithelial Cells |
|||
ABC-TC3695 | AcceGen | 1 vial | Ask for price |
Description: Human Mammary Luminal Epithelial Cell |
Human Ovarian Surface Epithelial Cells |
|||
ABC-TC3743 | AcceGen | 1 vial | Ask for price |
Description: The human ovary is covered with a single layer of flat to cuboidal surface epithelial cells (OSE). OSE are physiologically involved in follicular rupture and the subsequent repair of the follicle wall during reproductive age. OSE exhibit a gland formation in coculture with endometrial stromal cells in an estrogen-rich environment. The phenotypic plasticity of these cells shares a mesenchymal property when they are cultured on two layers of extracellular matrix and collagen gel. OSE are the source of epithelial ovarian carcinomas. Understanding the mechanisms that regulate OSE cell survival, apoptosis, and cell growth is of great clinical importance because an aberrant signaling of any of these pathways is likely to be involved in epithelial ovarian cancer.HOSEpiC from Gentaur Research Laboratories are isolated from human ovarian tissue. HOSEpiC are cryopreserved either at primary or passage one cultures and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HOSEpiC are characterized by immunofluorescent method with cytokeratine antibodies (CK-14, -18 and -19). HOSEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOSEpiC are guaranteed to further expand for over 5 population doublings in the conditions provided by Gentaur Research Laboratories. |
Human Retinal Pigment Epithelial Cells |
|||
ABC-TC3791 | AcceGen | 1 vial | Ask for price |
Description: The human retina is a multi-layered structure lining the rear of the eye. It consists of photoreceptor cells and retinal pigment epithelial cells (RPE). RPE situated between the neurosensory retina and the choroid, form the outer blood-retina barrier that controls the chemical composition of the subretinal space. RPE has long been recognized to differ from many other epithelia in the polarity of a number of proteins and whereas almost all other epithelia face an apical lumen devoid of matrix, the RPE apical plasma membrane is in contact with the extracellular matrix. During early embryonic development, RPE spontaneously transdifferentiate into neural retinal tissue. RPE play a critical role in the development and maintenance of adjacent photoreceptors. They generate a number of reactive oxygen species when illuminated with light and their proliferation is an important step in the pathogenesis of ocular diseases such as proliferative retinopathy. HRPEpiC from Gentaur Research Laboratories are isolated from human retina. HRPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HRPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HRPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRPEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories. |
Human Seminal Vesicle Epithelial Cells |
|||
ABC-TC3795 | AcceGen | 1 vial | Ask for price |
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Gentaur complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established. |
Human Small Intestine Epithelial Cells |
|||
ABC-TC3814 | AcceGen | 1 vial | Ask for price |
Description: Human small intestines cells are derived from whole small intestines that have been dissociated into multi-cellular aggregates and single cells then frozen. Human whole small intestines cells are from a single donor. The small intestine consists of several specialized epithelial cells that could help researchers determine the absorption potential of drug candidates. In addition, these cells can be used with studies of transport and metabolism of drugs and dietary chemicals. |
L’agoniste CD40 ciblé sur la protéine α d’activation des fibroblastes se synergise avec la radiothérapie dans les tumeurs murines de la tête et du cou HPV-positives
Goal: The incidence of human papillomavirus-associated head and neck squamous cell carcinoma (HPV+-HNSCC) is rising worldwide and though present therapeutic modalities are environment friendly within the majority of sufferers, there’s a excessive price of therapy failures. Thus, novel mixture approaches are urgently wanted to attain higher illness management in sufferers with HPV+-HNSCC. We investigated the security and therapeutic efficacy of a novel fibroblast activation protein (FAP)-targeted CD40 agonist (FAP-CD40) together with native hypofractionated radiation in a syngeneic HPV+-HNSCC mannequin.
Experimental design: Utilizing an established orthotopic mannequin, we handled tumor-bearing mice with native hypofractionated radiotherapy (2 × 6 Gy) alone or together with a systemic administration of the FAP-CD40 antibody. Following up the mice, we evaluated the adjustments within the tumor microenvironment (TME) by immunofluorescence, FACS, and NanoString RNA evaluation.
Outcomes: The suboptimal radiotherapy routine chosen failed to manage tumors within the handled mice. The FAP-CD40 administered in monotherapy transiently managed tumor development, whereas the mixed remedy induced sturdy full responses in additional than 80% of the tumor-bearing mice. This notable efficacy relied on the radiotherapy-induced transforming of the TME and activation of the CD8+ T-cell-cDC1 axis and was devoid of the systemic toxicity regularly related to CD40-targeted remedy. Furthermore, the strong immunologic reminiscence developed successfully prevented tumor relapses, a typical function in sufferers with HNSCC.
Conclusions: Our examine offers proof of idea, in addition to mechanistic insights of the therapeutic efficacy of a bispecific FAP-CD40 mixed with native radiotherapy in a FAP+-HNSCC mannequin rising general survival and inducing long-term antitumor immunity.
a restriction tumorale par le collagène de kind I s’oppose aux effets de promotion des tumeurs des fibroblastes associés au most cancers
Most cancers-associated fibroblasts (CAF) might exert tumor-promoting and tumor-suppressive features, however the mechanisms underlying these opposing results stay elusive. Right here, we sought to grasp these doubtlessly opposing features by interrogating purposeful relationships between CAF subtypes, their mediators, desmoplasia and tumor development in wide selection of tumor varieties metastasizing to the liver, the commonest organ website for metastasis.
Depletion of hepatic stellate cells (HSC), which represented primary supply of CAF in mice and sufferers in our examine, or depletion of all CAF decreased tumor development and mortality in desmoplastic colorectal and pancreatic metastasis, however not in non-desmoplastic metastatic tumors. Single cell RNA-sequencing together with CellPhoneDB ligand-receptor evaluation, in addition to research in immune cell-depleted and HSC-selective knockout mice uncovered direct CAF-tumor interactions as tumor-promoting mechanism, mediated by myofibroblastic CAF (myCAF)-secreted hyaluronan and inflammatory CAF (iCAF)-secreted HGF.
These results had been opposed by myCAF-expressed type-I collagen, which suppressed tumor development by mechanically restraining tumor unfold, overriding its personal stiffness-induced mechanosignals. In abstract, mechanical restriction by type-I collagen opposes the general tumor-promoting results of CAF, thus offering a mechanistic rationalization for his or her twin features in most cancers. Therapeutic focusing on of tumor-promoting CAF mediators whereas preserving type-I collagen might convert CAF from tumor-promoting to tumor-restricting.
Évaluation clinique, biométrique et échographique des effets de la greffe de fibroblastes autologues sur les rides du pli nasolabial
Background: Feeling stunning and staying younger have all the time been necessary to the individuals. Due to this fact, an in depth physique of analysis has centered on the efforts made to take away the pores and skin issues, particularly wrinkles. Fibroblast cells of the pores and skin are the assorted autologous cells at present utilized in repairing a number of wounds, scars, and pores and skin getting old. Thus, the current examine was carried out to evaluate the efficacy of the transplantation of the fibroblast cells in eliminating the facial wrinkles utilizing the biometric evaluation and to optimize the utility of this system on this therapy.
Strategies: The current examine was carried out on 22 male and feminine sufferers aged between 35 – 60 years previous. Samples had been collected from the retro-auricular area, and the fibroblast cells had been remoted and cultured. Topics acquired three injections with autologous fibroblasts at 2-week intervals. The sufferers had been adopted up for six months and structural adjustments of their wrinkles had been assessed by the sonography in addition to the VisioFace software program, cutometer, tewameter, and colorimeter.
Outcomes: The outcomes obtained utilizing the VisioFace software program confirmed the numerous phenotypic adjustments within the sufferers after the nasolabial injections (81.42±23.97 vs. 60.91±21.91, P=0.0001). The outcomes confirmed a major enhance within the complete pores and skin density (13.73±6.30 vs. 26.27±7.93, P=0.0001), dermis density (11.28±5.21 vs. 31.88±7.96, P=0.0001), dermis density (27.68±23.15 vs. 49.21±45.68, P=0.046), and dermis thickness (798.09±133.51 μm vs. 905.59±240.67 μm, P=0.036) in comparison with pre-treatment.
Conclusion: The findings of the examine revealed that the injection of autologous fibroblasts could be efficient in restoring the getting old pores and skin, particularly within the nasolabial area, and can be utilized as a secure rejuvenating technique.
Fibroblasts Antibody |
|||
GWB-833798 | GenWay Biotech | 0.1 mg | Ask for price |
Human Skin Fibroblasts |
|||
HXXFB-00001 | Cyagen | 1*10^6/vial | EUR 1529 |
Human Liver Fibroblasts |
|||
ABC-TC3672 | AcceGen | 1 vial | Ask for price |
Description: Human Liver Fibroblasts are isolated from normal human liver tissue. Human Liver Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml. |
Human Fibroblasts, Dermis |
|||
ABC-TC3622 | AcceGen | 1 vial | Ask for price |
Description: The HFIB-D cultures are produced on the base of human tissues. The HFIB-D are isolated according to known procedures. Then primary cultures are produced. After further culturing, the HFIB-D are frozen in a computer controlled freezing unit. |
Human Tonsil Fibroblasts |
|||
ABC-TC3835 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Fibroblasts are among the most accessible normal mammalian cell types and still the most amenable to culture in vitro.HTF from Gentaur Research Laboratories are isolated from human tonsils. HTF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HTF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HTF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Kidney Fibroblasts |
|||
ABC-TC5519 | AcceGen | 1 vial | Ask for price |
Description: Human Kidney Fibroblasts are isolated from the human adult healthy renal tissue. HKF-a are cryopreserved after passage one culture and delivered frozen. HKF-a are guaranteed to further expand for 15 population doublings under the conditions provided by in the technical sheet.As an excretory organ in the urinary system, the kidney greatly influences an individual’s well being by preserving homeostasis through regulating electrolytes, pH, and blood pressure among other control mechanisms. Comprising the majority of interstitial cells in the kidney, renal fibroblasts are responsible for the synthesis of collagen I of the extracellular matrix. Also, renal cortical fibroblasts synthesize erythropoietin, an important glycoprotein hormone that controls the production of red blood cells and renal medullary fibroblasts produce prostaglandins, a class of autocrine or paracrine hormones, which help maintain water and electrolyte homeostasis. Renal interstitial fibrosis, a disorder that may escalate to chronic kidney disease, is often correlated with excessive deposition of extracellular matrix by renal fibroblasts, the regulation of which process is under intensive research. |
Human Kidney Fibroblasts |
|||
CSC-8240W | Creative Bioarray | One Frozen vial | Ask for price |
Human Dermal Fibroblasts |
|||
CSC-C0519Z | Creative Bioarray | One Frozen vial | Ask for price |
Human Spleen Fibroblasts |
|||
CSC-C4868L | Creative Bioarray | One Frozen vial | Ask for price |
Native Human Fibroblasts |
|||
MBS556459-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Human Adrenal Fibroblasts |
|||
ABC-TC3478 | AcceGen | 1 vial | Ask for price |
Description: The adrenal gland plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones. Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.HAdF from Gentaur Research Laboratories are isolated from human adrenal tissue. HAdF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HAdF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HAdF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAdF are guaranteed to further expand for 15 population doublings under the conditions provided by Gentaur Research Laboratories. |
Human Cardiac Fibroblasts |
|||
ABC-TC3533 | AcceGen | 1 vial | Ask for price |
Description: The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors.HCF from Gentaur Research Laboratories are isolated from the human heart. HCF-a are cryopreserved at secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCF-a are characterized by immunofluorescent method with antibody to fibronectin. HCF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-a are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Mammary Fibroblasts |
|||
ABC-TC3694 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are the most common types of mammary stromal cells. Mammary stromal cells differentiate into fibroblasts, adipocytes or vascular structures in a hormone- and substatum-dependent manner, and may explain the dramatic changes in stromal composition during both normal mammary gland development and tumorigenesis. The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. The Mammary stromal fibroblasts express and produce cytokines such as SF witch plays roles in mammary cancer. HMF from Gentaur Research Laboratories are isolated from human mammary tissue. HMF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HMF are characterized by immunofluorescent method with antibodies to fibronectin, CD90 and vimentin. HMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMF are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories. |
Human Ovarian Fibroblasts |
|||
ABC-TC3741 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Ovarian fibroblasts stimulate human ovarian cancer cell invasion and expression of 72-kDa gelatinase A (MMP-2).HOF from Gentaur Research Laboratories are isolated from human ovary tissue. HOF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HOF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HOF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Splenic Fibroblasts |
|||
ABC-TC3818 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. There is good evidence that fibroblasts in different parts of the body are intrinsically different and display different regulatory functions on surrounding cells. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. HSF from Gentaur Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Thyroid Fibroblasts |
|||
ABC-TC3832 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Because the thyroid is easily susceptible to inflammatory autoimmune diseases that can result in tissue damage, thyroid fibroblasts may play an important role in regulating thyroid function and reaction to disease. HThF from Gentaur Research Laboratories are isolated from human thyroid tissue. HThF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HThF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HThF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HThF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Uterine Fibroblasts |
|||
ABC-TC3863 | AcceGen | 1 vial | Ask for price |
Description: Derived from the mesoderm, connective tissue is involved in maintaining the structural integrity of the body. The predominant cells found in connective tissue are fibroblasts, which continuously secrete various components of the extracellular matrix. Depending on the site of origin and physiological status, fibroblasts may exhibit different morphological phenotypes and various functional properties.Gentaur offers a range of Human Fibroblasts produced at Gentaur's cell culture facility. The cells are isolated from normal human juvenile foreskin or adult skin from different locations. In addition, Gentaur offers Fibroblasts from adult lung tissue, aortic adventitial tissue, and villous placental tissue.Shortly after isolation, all Gentaur Human Fibroblasts are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Human Gastric Fibroblasts |
|||
ABC-TC5516 | AcceGen | 1 vial | Ask for price |
Description: Gastric fibroblasts (GF), which are located in the stomach, are mesenchymal cells derived from the embryonic mesoderm. Fibroblasts secrete a non-rigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. Studies in vitro and in vivo show that GF can regulate epithelial regeneration by influencing epithelial cell proliferation and differentiation. In addition to their role in wound healing, human GF (HGF) have been shown to be key contributors in the development and progression of gastric carcinoma. HGF are a useful model for examining the underlying mechanisms that cause gastric carcinoma and may provide novel insights for developing new cancer drug therapies. |
Human Splenic Fibroblasts |
|||
CSC-C9230J | Creative Bioarray | One Frozen vial | Ask for price |
Human Thyroid Fibroblasts |
|||
CSC-7702W | Creative Bioarray | One Frozen vial | Ask for price |
Human Colonic Fibroblasts |
|||
CSC-8242W | Creative Bioarray | One Frozen vial | Ask for price |
Human Hepatic Fibroblasts |
|||
CSC-C1500 | Creative Bioarray | One Frozen vial | Ask for price |
Human Cardiac Fibroblasts |
|||
CSC-C1503 | Creative Bioarray | One Frozen vial | Ask for price |
Human Mammary Fibroblasts |
|||
CSC-C1527 | Creative Bioarray | One Frozen vial | Ask for price |
Human Gingival Fibroblasts |
|||
ABC-TC3627 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells with many vital functions during development and in adult organisms. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Fibroblasts are among the most accessible normal mammalian cell types and still the most amenable to culture in vitro. Gingival fibroblasts are the major constituents of gingival tissue and play a key role in their health maintenance. Human gingival fibroblasts (HGnF) express a wide surface molecular panel including mainly CD9, CD26, CD55, CD59, CD63, CD71, CD86 CD95, CD99 and CD117. They were also demonstrated to express mRNAs for protease-activated receptor-1 (PAR-1) and PAR-3.HGnF from Gentaur Research Laboratories are isolated from human gingiva. HGnF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HGnF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HGnF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGnF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Prostate Fibroblasts |
|||
ABC-TC3765 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of the easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Cultured prostate fibroblasts synthesize FGF-like growth factor that stimulate their growth and is believed to be important in the development of benign prostatic hyperplasia.HPrF from Gentaur Research Laboratories are isolated from human prostate tissue. HPrF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPrF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HPrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPrF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Prostate Fibroblasts |
|||
ALHE28 | Alphabioregen | 1ml frozen Vial | EUR 620 |
Human Prostate Fibroblasts |
|||
CSC-7730W | Creative Bioarray | One Frozen vial | Ask for price |
Human Gingival Fibroblasts |
|||
cAP-0140 | Angio Proteomie | 1Frozen Vial | EUR 693 |
Human pancreas fibroblasts |
|||
P0018025 | Addexbio | One Frozen vial | EUR 580 |
Human Bronchial Fibroblasts |
|||
ABC-TC3529 | AcceGen | 1 vial | Ask for price |
Description: Human Bronchial Fibroblasts from Gentaur Research Laboratories are isolated from human bronchus tissue. HBF are cryopreserved at secondary culture (P1) and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBF are characterized by immunofluorescent method with antibody to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Lymphatic Fibroblasts |
|||
ABC-TC3688 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Besides, dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.HLF from Gentaur Research Laboratories are isolated from human lymph nodes. HLF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Pulmonary Fibroblasts |
|||
ABC-TC3776 | AcceGen | 1 vial | Ask for price |
Description: The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. HPF from Gentaur Research Laboratories are isolated from human lung tissue. HPF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPF are characterized by immunofluorescent method with antibody to fibronectin. HPF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Intestine Fibroblasts |
|||
cAP-0206 | Angio Proteomie | 1Frozen Vial | EUR 544.5 |
Human Esophageal Fibroblasts |
|||
ABC-TC3613 | AcceGen | 1 vial | Ask for price |
Description: Esophageal fibroblasts which line the lamina propria of the upper and lower esophagus are mesenchymal cells that originated from the embryonic mesoderm. They have been shown to play an important role in angiogenesis through TGF-β and VEGF signaling pathways in the highly angiogenic esophageal squamous cell carcinoma. Predominantly, fibroblasts secrete a nonrigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in the connective tissues and play major roles in wound healing. Because they are one of the simplest types of cells to grow in culture fibroblasts have been extensively used for a wide range of cellular and molecular studies. In addition, fibroblasts’ durability makes them amenable to a wide variety of manipulations ranging from gene transfection to microinjection.The HEF cells from Gentaur Research Laboratories are isolated from human esophageal tissue. HEF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEF are characterized by immunofluorescence with antibody specific to fibronectin. HEF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEF are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories. |
Human Intestinal Fibroblasts |
|||
ABC-TC3655 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.HIF from Gentaur Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Pancreatic Fibroblasts |
|||
ABC-TC5470 | AcceGen | 1 vial | Ask for price |
Description: Human Primary Pancreatic Fibroblasts are isolated from normal human pancreatic tissue. Human Primary Pancreatic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml. |
Human Pancreatic Fibroblasts |
|||
CSC-C4882L | Creative Bioarray | One Frozen vial | Ask for price |
Human Pancreatic Fibroblasts |
|||
cAP-0144 | Angio Proteomie | 1Frozen Vial | EUR 693 |
Human Gallbladder Fibroblasts |
|||
ABC-TC3625 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. Because they are one of the easiest types of cells to grow in culture, they have been extensively used for a wide range of cellular and molecular studies. Additionally, their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Human gallbladder fibroblasts (HGBF) are responsible for eicosanoid synthesis, specifically PGI2. Chronic stimulation of HGBF results in up-regulated synthesis and release of PGI2 which aids in formation of early acute cholecystitis. HGBF are very versatile cells and could be used in studies of many conditions, such as the early stages of acute cholecystitis.HGBF from Gentaur Research Laboratories are isolated from human gallbladder tissue. HGBF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HGBF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HGBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGBF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Pericardial Fibroblasts |
|||
ABC-TC3748 | AcceGen | 1 vial | Ask for price |
Description: Pericardial Fibroblasts are the principal cell type of the serous pericardium. They help to form the strong connective tissue that allows the pericardium to serve as a protective sac for the heart. Pericardial fibroblasts are exposed to a dynamic mechanical environment, being attached to the diaphragm, which influences the structure integrity of both healthy and healing tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen and there is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts have been extensively used for a wide range of cellular and molecular studies because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.HPcF from Gentaur Research Laboratories are isolated from the human heart. HPcF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPcF are characterized by immunofluorescent method with antibody to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPcF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Parathyroid Fibroblasts |
|||
cAP-0218 | Angio Proteomie | each | EUR 782.1 |
Immortalized Human Fibroblasts |
|||
ABI-TC052D | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are the most common cells of mammalian connective tissue. AcceGen offers an Immortalised fibroblast cell line originated from the human foreskin. |
Human Conjunctival Fibroblasts |
|||
ABC-TC3574 | AcceGen | 1 vial | Ask for price |
Description: The fibroblast is the cellular element responsible for fibrosis. Conjunctival fibroblasts, as other fibroblasts, act not only as structural cells in the production of the matrix components, but are also actively involved as effectors in allergic conjunctivitis. Conjunctival fibroblasts cultured with histamine induce the production of proinflammatory cytokines, such as IL-1, IL-6 and IL-8. Conjunctival fibroblast culture can serve as a useful in vitro model for rapid screening of drugs for their effect on fibroblast morphology, motility, and proliferation, and provide a basis for the development of new treatments for a variety of ocular proliferative disorders.HConF from Gentaur Research Laboratories are isolated from human conjunctiva. HConF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HConF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HConF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HConF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
TIMP-2, human fibroblasts |
|||
7788-5 | Biovision | each | EUR 405.6 |
Human Lung Fibroblasts, Adult |
|||
ABC-TC3683 | AcceGen | 1 vial | Ask for price |
Description: Human Lung Fibroblasts (HLF) are derived from normal human lung parenchyma. They are cryopreserved at first passage and can be cultured and propagated at least 12 population doublings. |
Human Skin Cells (Fibroblasts) |
|||
ABC-TC3807 | AcceGen | 1 vial | Ask for price |
Description: Human fibroblasts are derived from cultured skin explants. These fibroblasts are from a single donor. |
Human Lung Fibroblasts, Foetal |
|||
ABC-TC3684 | AcceGen | 1 vial | Ask for price |
Description: Human Lung Fibroblasts (HLF) are derived from normal human lung parenchyma. They are cryopreserved at first passage and can be cultured and propagated at least 12 population doublings. |
Human Dermal Fibroblasts (HDFa) |
|||
P0020022 | Addexbio | One Frozen vial | EUR 420 |
Fibroblasts/Epithelial Cells Antibody |
|||
abx413035-02mg | Abbexa | 0.2 mg | EUR 777.6 |
Fibroblasts/Epithelial Cells Antibody |
|||
abx413036-200l | Abbexa | 200 µl | EUR 200 |
Fibroblasts/Epithelial Cells Antibody |
|||
abx413036-25ug | Abbexa | 25 ug | EUR 360 |
Fibroblasts/Epithelial Cells Antibody |
|||
GWB-Q01116 | GenWay Biotech | 0.2 mg | Ask for price |
Fibroblasts/Epithelial Cells Antibody |
|||
GWB-Q01117 | GenWay Biotech | 25 ug | Ask for price |
Human Dermal Fibroblasts, Adult |
|||
ABC-TC3029 | AcceGen | 1 vial | Ask for price |
Description: Human Dermal Fibroblasts-Adult are isolated from adult human skin. Human Dermal Fibroblasts-Adult are cryopreserved at P0 and delivered frozen. Human Dermal Fibroblasts-Adult are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. Human Dermal Fibroblasts-Adult are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.Human Dermal Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency. |
Human Dermal Fibroblasts, Fetal |
|||
ABC-TC3588 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. In addition, dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype.HDF from Gentaur Research Laboratories are isolated from fetal human skin. HDF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HDF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HDF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HDF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Vas Deferens Fibroblasts |
|||
ABC-TC3867 | AcceGen | 1 vial | Ask for price |
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Gentaur complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established. |
Human Dermal Fibroblasts (Adult) |
|||
cAP-0008-ad | Angio Proteomie | 1Frozen Vial | EUR 445.5 |
Human Dermal Fibroblasts, Pooled |
|||
ABC-TC3399 | AcceGen | 1 vial | Ask for price |
Description: For routine cell cultivation, We recommends to work without antibiotics / antimycotics. For isolation, However, we recommend the use of antibiotics / antimycotics up to passage 2. |
Human Lung Fibroblasts-Adults |
|||
cAP-0033Ad | Angio Proteomie | 1Frozen Vial | EUR 594 |
Human Choroid Plexus Fibroblasts |
|||
ABC-TC3569 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells, which they derived from the embryonic mesoderm. Although cells loosely called fibroblasts have been isolated from many tissues and assumed to be connective tissue cells, fibroblasts appear to be the least specialized cells. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Fibroblasts are the easiest of cells to grow in culture-a feature that has made them a favorite subject for cell biological studies.HCPF from Gentaur Research Laboratories are isolated from human choroid pluxus. HCPF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCPF are characterized by immunofluorescent method with antibody to fibronectin. HCPF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCPF are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories. |
Human Lymphatic Fibroblasts Cell |
|||
ABC-TC3689 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. |
Human Ocular Choroid Fibroblasts |
|||
ABC-TC3729 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They are one of the easiest types of cells to grow in culture and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. During wound healing, fibroblasts switch from a migrator, repopulation phenotype to a contractile, matrix-reassembling phenotype. Choroid fibroblasts are exposed to a dynamic environment that influences cellular VEGF expression, which plays a significant role in angiogenesis and macular degeneration.HOCF from Gentaur Research Laboratories are isolated from human ocular choroid tissue. HOCF are cryopreserved at primary culture. Each vial contains 5×10^5 cells in 1 ml volume. HOCF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HOCF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOCF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Dermal Fibroblasts-fetal |
|||
CSC-7797W | Creative Bioarray | One Frozen vial | Ask for price |
Human Dermal Fibroblasts, Neonatal |
|||
ABC-TC3451 | AcceGen | 1 vial | Ask for price |
Description: Human Dermal Fibroblasts-Neonatal are isolated form neonatal human skin. Human Dermal Fibroblasts-Neonatal are cryopreserved at P1 and delivered frozen. Human Dermal Fibroblasts-Neonatal are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. Human Dermal Fibroblasts-Neonatal are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. |
Human Bladder Stromal Fibroblasts |
|||
ABC-TC3515 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of the easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechanical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. As cells of mesenchymal origin, human bladder stromal fibroblasts (HBdSF) also secrete FGF-7, which interact primarily with bladder epithelial cells. FGF-7 secreted by HBdSF is essential for stimulating the formation of a multilayered stratified bladder epithelium. HBdSF from Gentaur Research Laboratories are isolated from human bladder tissue. HBdSF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBdSF are characterized by immunofluorescent method with antibody to fibronectin. HBdSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Dermal Fibroblasts (Neonatal) |
|||
cAP-0008-neo | Angio Proteomie | 1Frozen Vial | EUR 445.5 |
Human Primary Uterine Fibroblasts |
|||
T4084 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Human Primary Cardiac Fibroblasts |
|||
T4038 | ABM | 5x10^5 cells / 1.0 ml | EUR 1375 |
Gaucher Disease Human Fibroblasts |
|||
T4135 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
Human Cardiac Fibroblasts-Atrial |
|||
ABC-TC3537 | AcceGen | 1 vial | Ask for price |
Description: Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or verticle. |
Human Fibroblasts-GFP Expressing |
|||
GFP07 | Neuromics | 500,000 Cells in T25 flask | EUR 1294.92 |
Human Fibroblasts-GFP Expressing |
|||
MBS556000-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Human Primary Tracheal Fibroblasts |
|||
T4005 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Human Primary Neonatal Fibroblasts |
|||
T4104 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Human Aortic Adventitia Fibroblasts |
|||
ABC-TC3490 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells which derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. The pulmonary arterial vesculature responds to various insults with a characteristic pattern of remodeling that includes an increase in connective tissue in the medial layer of the vessel wall. One of the pathogenetic mechanisms involved is the functional derangements of vascular fibroblasts. Their proliferative response contributes to the adventitial thickening observed during the development of hypoxia-induced hypertension. HAAF from Gentaur Research Laboratories are isolated from human aortic artery. HAAF are cryopreserved at first passage of primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HAAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HAAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAAF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Bladder Fibroblasts, Secondary |
|||
ABC-TC3512 | AcceGen | 1 vial | Ask for price |
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Gentaur complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established. |
Human Primary Epidermal Fibroblasts |
|||
T0300 | ABM | 5x10^5 cells / 1.0 ml | EUR 625 |
Human Primary Pulmonary Fibroblasts |
|||
T4009 | ABM | 5x10^5 cells / 1.0 ml | EUR 975 |
Fibroblasts/Epithelial Cells Antibody (PE) |
|||
abx413038-200l | Abbexa | 200 µl | EUR 250 |
Human Aortic Adventitial Fibroblasts |
|||
ABC-TC3033 | AcceGen | 1 vial | Ask for price |
Description: Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or verticle. |
Human Primary Esophageal Fibroblasts |
|||
T4232 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Fibroblasts |
|||
MBS6507500-01mg | MyBiosource | 0.1mg | EUR 790 |
Fibroblasts |
|||
MBS6507500-5x01mg | MyBiosource | 5x0.1mg | EUR 3395 |
Mouse Anti Human Fibroblasts/Epithelial Cells Monoclonal Antibody,RPE |
|||
DMABT-49595MH | Creative Diagnostics | 25 TEST | EUR 382.2 |
Description: Mouse |
Fibroblasts/Epithelial Cells Antibody (RPE) |
|||
abx413037-100tests | Abbexa | 100 tests | EUR 811.2 |
Fibroblasts/Epithelial Cells Antibody (RPE) |
|||
abx413038-25tests | Abbexa | 25 tests | EUR 427.2 |
Fibroblasts / Epithelial Cells Antibody (RPE) |
|||
GWB-2B065D | GenWay Biotech | 100 TESTS | Ask for price |
Fibroblasts / Epithelial Cells Antibody (RPE) |
|||
GWB-F0EF95 | GenWay Biotech | 25 TESTS | Ask for price |
Human Peridontal Ligament Fibroblasts |
|||
ABC-TC3750 | AcceGen | 1 vial | Ask for price |
Description: The periodontal ligament is a connective tissue located between the cementum of teeth and the alveolar bone of the mandibula. It plays an integral role in the maintenance and regeneration of periodontal tissue. The cells responsible for maintaining this tissue are thought to be fibroblasts, which can be either multipotent or composed of heterogenous cell populations that differ in their function. Despite the similar morphology with gingival fibroblasts, periodontal ligament fibroblasts (PDLF) appear to display distinct functional activities in the maintenance of tissue integrity. It is known that PDLF produce osteoblast-related extracellular matrix proteins and show higher alkaline phosphatase activity compared with gingival fibroblasts. PDLF actively participate in immune and inflammatory events in periodontal diseases.HPDLF from Gentaur Research Laboratories are isolated from human periodontal tissue. HPDLF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPDLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HPDLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPDLF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Villous Mesenchymal Fibroblasts |
|||
ABC-TC3869 | AcceGen | 1 vial | Ask for price |
Description: Derived from the mesoderm, connective tissue is involved in maintaining the structural integrity of the body. The predominant cells found in connective tissue are fibroblasts, which continuously secrete various components of the extracellular matrix. Depending on the site of origin and physiological status, fibroblasts may exhibit different morphological phenotypes and various functional properties.Gentaur offers a range of Human Fibroblasts produced at Gentaur's cell culture facility. The cells are isolated from normal human juvenile foreskin or adult skin from different locations. In addition, Gentaur offers Fibroblasts from adult lung tissue, aortic adventitial tissue, and villous placental tissue.Shortly after isolation, all Gentaur Human Fibroblasts are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur. |
Immortalized Human Cardiac Fibroblasts |
|||
CSC-I9134L | Creative Bioarray | One Frozen vial | Ask for price |
Human Periodontal Ligament Fibroblasts |
|||
CSC-C4607J | Creative Bioarray | One Frozen vial | Ask for price |
Immortalized Human Uterine Fibroblasts |
|||
T0610 | ABM | 1x106 cells / 1.0 ml | EUR 2750 |
Immortalized Human Cardiac Fibroblasts |
|||
T0446 | ABM | 1x106 cells / 1.0 ml | EUR 3950 |
MMP-1 proenzyme (human fibroblasts) |
|||
MBS566029-0005mg | MyBiosource | 0.005mg | EUR 400 |
MMP-1 proenzyme (human fibroblasts) |
|||
MBS566029-5x0005mg | MyBiosource | 5x0.005mg | EUR 1750 |
MMP-2 proenzyme (human fibroblasts) |
|||
MBS566030-0005mg | MyBiosource | 0.005mg | EUR 375 |
MMP-2 proenzyme (human fibroblasts) |
|||
MBS566030-5x0005mg | MyBiosource | 5x0.005mg | EUR 1630 |
TIMP-2 (human fibroblasts) (purified) |
|||
MBS566662-0005mg | MyBiosource | 0.005mg | EUR 425 |
TIMP-2 (human fibroblasts) (purified) |
|||
MBS566662-5x0005mg | MyBiosource | 5x0.005mg | EUR 1875 |
Human Cardiac Fibroblasts-Ventricular |
|||
ABC-TC3539 | AcceGen | 1 vial | Ask for price |
Description: Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or verticle. |
Immortalized Human Foreskin Fibroblasts |
|||
CSC-I2034Z | Creative Bioarray | One Frozen vial | Ask for price |
Human Skin Cells (Fibroblasts, Prenata) |
|||
ABC-TC3809 | AcceGen | 1 vial | Ask for price |
Description: Human fibroblasts are derived from cultured skin explants. Human fibroblasts are from a single donor. These cells enable researchers to study skin diseases such as dermatitis, wound healing, and other diseases that are expressed in fibroblast. In addition, they may be used to study the development of skin and production of inducible pluripotent stem cells. Development period: Prenata |
Human MMC treated Dermal Fibroblasts |
|||
ABC-TC3983 | AcceGen | 1 vial | Ask for price |
Description: MCFibs provide an ideal cell system to establish human feeder layers for the culture of embryonic stem cells or other cell types requiring a feeder layer for optimal proliferation. MCFibs are quality tested in LIFib's Serum-Free Medium to ensure high viability and plating efficiency. Cell Features:MCFibs are cryopreserved after secondary culture and treatment with MMC.MCFibs remain viable and attached in serum-free, animal protein-free, phenol red-free, and antimicrobial-free medium.MCFibs are extensively tested for quality and optimal performance.MCFibs are ready to use.MCFibs are pretreated to help reduce toxic agents in your lab.Gentaur guarantees performance and quality.MCFibs are tested for suitability as feeder layers for human embryonic stem cell cultures. |
Complete Medium For Human Fibroblasts |
|||
HXXFB-90011 | Cyagen | 500mL | EUR 250 |
Human Skin Cells (Fibroblasts, Postnatal) |
|||
ABC-TC3808 | AcceGen | 1 vial | Ask for price |
Description: Human fibroblasts are derived from cultured skin explants. Human fibroblasts are from a single donor. These cells enable researchers to study skin diseases such as dermatitis, wound healing, and other diseases that are expressed in fibroblast. In addition, they may be used to study the development of skin and production of inducible pluripotent stem cells. Development period: Postnatal |
Human Dermal Fibroblasts - Stretch Marks |
|||
LOCC-7221 | Westburg | each | Ask for price |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS |
|||
MBS214991-02mg | MyBiosource | 0.2mg | EUR 550 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS |
|||
MBS214991-5x02mg | MyBiosource | 5x0.2mg | EUR 2295 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS |
|||
MBS214992-0025mg | MyBiosource | 0.025mg | EUR 225 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS |
|||
MBS214992-5x0025mg | MyBiosource | 5x0.025mg | EUR 835 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE |
|||
MBS214993-100Tests | MyBiosource | 100Tests | EUR 575 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE |
|||
MBS214993-5x100Tests | MyBiosource | 5x100Tests | EUR 2410 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE |
|||
MBS214994-25Tests | MyBiosource | 25Tests | EUR 270 |
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE |
|||
MBS214994-5x25Tests | MyBiosource | 5x25Tests | EUR 1035 |
Human Cardiac Fibroblasts-Adult Atrial |
|||
ABC-TC3535 | AcceGen | 1 vial | Ask for price |
Description: The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors.HCFaa from Gentaur Research Laboratories are isolated from the human heart. HCF-aa are cryopreserved at secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCF-aa are characterized by immunofluorescent method with antibody to fibronectin. HCF-aa are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-aa are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Cardiac Fibroblasts-Fetal Atrial |
|||
ABC-TC3538 | AcceGen | 1 vial | Ask for price |
Description: Cardiac fibroblasts (CF) provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction and cardiac fibrosis. CF cultures have been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors. CF also has been found to form electric coupling with myocytes through gap junctions exerting electrotonic influences on the cells. These electronic couplings are suggested to play an important role in genesis of complex fractionated atrial electrogram sites and thus are a perfect model for study of atrial fibrillation.HCF-fa from Gentaur Research Laboratories are isolated from the human fetal atrium. HCF-fa are cryopreserved at secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCF-fa are characterized by immunofluorescence with antibody specific to fibronectin. HCF-fa are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-fa are guaranteed to further expand for 15 population doublings under the conditions provided by Gentaur Research Laboratories. |
Human Cardiac Fibroblasts-fetal atrial |
|||
CSC-7818W | Creative Bioarray | One Frozen vial | Ask for price |
Human Primary Bladder Stromal Fibroblasts |
|||
T4162 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Immortalized Human Liver Fibroblasts - SV40 |
|||
T9450 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Human iPSC Line from human dermal fibroblasts |
|||
CSC-00851L | Creative Bioarray | One Frozen vial | Ask for price |
Human Cardiac Fibroblasts-Adult Ventrical |
|||
ABC-TC3536 | AcceGen | 1 vial | Ask for price |
Description: The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors.HCFav from Gentaur Research Laboratories are isolated from the human heart. HCF-av are cryopreserved at secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCFav are characterized by immunofluorescent method with antibody to fibronectin. HCF-av are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-av are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Colon Cancer-Associated Fibroblasts |
|||
CSC-C2847 | Creative Bioarray | One Frozen vial | Ask for price |
Human Cardiac Fibroblasts-adult ventrical |
|||
CSC-C4872L | Creative Bioarray | One Frozen vial | Ask for price |
Immortalized Human Corneal Fibroblasts (IHCF) |
|||
T0578 | ABM | 1x106 cells / 1.0 ml | EUR 3950 |
Human Brain Vascular Adventitial Fibroblasts |
|||
ABC-TC3525 | AcceGen | 1 vial | Ask for price |
Description: Human Brain Vascular Adventitial Fibroblasts from Gentaur are isolated from human brain vascular tissue. HBVAF are cryopreserved at first passage of primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBVAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HBVAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBVAF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Immortalized Human Kidney Fibroblasts-SV40 |
|||
CSC-I2121Z | Creative Bioarray | One Frozen vial | Ask for price |
Immortalized Human Mammary Fibroblasts (HMF3A) |
|||
T0153 | ABM | 1x106 cells / 1.0 ml | EUR 3950 |
Immortalized Human Mammary Fibroblasts (HMF3S) |
|||
T0154 | ABM | 1x106 cells / 1.0 ml | EUR 3950 |
Immortalized Human Diploid Fibroblasts - hTERT |
|||
T1013 | ABM | 1x106 cells / 1.0 ml | EUR 2750 |
Human Pulmonary Artery Adventitia Fibroblasts |
|||
ABC-TC3773 | AcceGen | 1 vial | Ask for price |
Description: Fibroblasts are mesenchymal cells which derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. The pulmonary arterial vesculature responds to various insults with a characteristic pattern of remodeling that includes an increase in connective tissue in the medial layer of the vessel wall. One of the pathogenetic mechanisms involved is the functional derangements of vascular fibroblasts. Their proliferative response contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. HPAAF from Gentaur Research Laboratories are isolated from human pulmonary artery. HPAAF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HPAAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HPAAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAAF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories. |
Human Breast Carcinoma Associated Fibroblasts |
|||
ABC-TC5536 | AcceGen | 1 vial | Ask for price |
Description: CAFs reside within tumor tissue and serve various functions including suppression of immune responses to cancerous cells, support of tumor cell growth and enhancement of metastasis.Product is manufactured, tested and validated in an ISO 9001/ISO13485/CLIA certified facility. |
Human Pulmonary Artery Adventitia Fibroblasts |
|||
CSC-C4028X | Creative Bioarray | One Frozen vial | Ask for price |
Immortalized Human Gingival Fibroblasts- hTERT |
|||
T0026 | ABM | 1x106 cells / 1.0 ml | EUR 3950 |
Immortalized Human Neonatal Fibroblasts - hTERT |
|||
T0344 | ABM | 1x106 cells / 1.0 ml | EUR 2750 |
Human Primary Villous Mesenchymal Fibroblasts |
|||
T4112 | ABM | 5x10^5 cells / 1.0 ml | Ask for price |
Immortalized Human Gingival Fibroblasts-SV40 |
|||
CSC-I2120Z | Creative Bioarray | One Frozen vial | Ask for price |
Immortalized Human Pulmonary Fibroblasts Cells |
|||
CSC-I9127L | Creative Bioarray | One Frozen vial | Ask for price |
Immortalized Human Pulmonary Fibroblasts Cells |
|||
T0490 | ABM | 1x106 cells / 1.0 ml | EUR 2750 |
Human Primary Periodontal Ligament Fibroblasts |
|||
T4075 | ABM | 5x10^5 cells / 1.0 ml | EUR 975 |
NHCF-A Human Cardiac Fibroblasts-Atrial |
|||
LOCC-2903 | Westburg | each | EUR 1960.39 |
Human Melanoma Carcinoma Associated Fibroblasts |
|||
ABC-TC5541 | AcceGen | 1 vial | Ask for price |
Description: Human Melanoma Cancer Associate Fibroblasts are isolated from malignant melanoma and cryopreserved at a low passage.Product is manufactured, tested and validated in an ISO 9001/ISO13485/CLIA certified facili |
Immortalized human Intestinal Fibroblasts-SV40 |
|||
CSC-I2043Z | Creative Bioarray | One Frozen vial | Ask for price |
Human Dermal Fibroblasts-fetal Feeder Cells |
|||
T2002 | ABM | 10^6 cells/1.0 ml | EUR 200 |
Human Primary Osteoarthritis Synovial Fibroblasts |
|||
T5408 | ABM | 5 x 10^5/ 1.0 ml | Ask for price |
OASA02809-25UG - FIBROBLASTS/EPITHELIAL CELLS Antibody |
|||
OASA02809-25UG | Aviva Systems Biology | 25ug | EUR 149 |
GFP Expressing Human Lung Fibroblasts-Adults |
|||
GF13 | Alphabioregen | 1ml frozen Vial | EUR 850 |
OASA02808-200UG - FIBROBLASTS/EPITHELIAL CELLS Antibody |
|||
OASA02808-200UG | Aviva Systems Biology | 0.2mg | EUR 499 |
GFP Expressing Human Dermal Fibroblasts-Adult |
|||
cAP-0008-adGFP | Angio Proteomie | 1Frozen Vial | EUR 782.1 |
RFP Expressing Human Dermal Fibroblasts-Adult |
|||
cAP-0008-adRFP | Angio Proteomie | 1Frozen Vial | EUR 782.1 |
Human Primary COPD Lung Parenchymal Fibroblasts |
|||
T5428 | ABM | 1 x 10^6 / 1.0 ml | Ask for price |
NHCF-V Human Cardiac Fibroblasts-Ventricular |
|||
LOCC-2904 | Westburg | each | EUR 1687.78 |
Rat Lung Fibroblasts |
|||
ABC-TC4158 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Lung Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Lung Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Lung Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Skin Fibroblasts |
|||
ABC-TC4217 | AcceGen | 1 vial | Ask for price |
Description: Rat skin fibroblasts, 2-week Wistar rat |
Rat Vein Fibroblasts |
|||
ABC-TC4240 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Vein Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Vein Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Vein Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Human Skin PrimaCell™: Normal Skin Fibroblasts |
|||
2-96103 | CHI Scientific | 1 Kit | EUR 1132.11 |
HighQC™ Human IPSC From Foreskin Fibroblast (Foreskin Fibroblasts) |
|||
ABC-SC0018T | AcceGen | 1 vial | Ask for price |
Description: iPSC (induced Pluripotent Stem cells) reprogrammed from human Foreskin FibroblastsThis iPS cell line was created using a normal human foreskin fibroblast. The iPS cell line was generated using an episomal reprogramming containing a prprietary mix of vectors, containing Oct-4, Sox-2, Klf-4 along with p53 Anti-sense, EBNA-1.Thus, a newborn foreskin fibroblast was reprogrammed into induced pluripotent stem cells (iPSC).The cell line was validated for pluripotency based on colony morphology, alkaline phosphatase expression, and expression of SSEA-4.iPS colonies exhibit classical morphology and growth.Absence of Mycoplasma was confirmed. |
GFP Expressing Human Dermal Fibroblasts-neonatal |
|||
cAP-0008-neoGFP | Angio Proteomie | 1Frozen Vial | EUR 782.1 |
RFP Expressing Human Dermal Fibroblasts-neonatal |
|||
cAP-0008-neoRFP | Angio Proteomie | 1Frozen Vial | EUR 782.1 |
GFP-Expressing Human Neonatal Dermal Fibroblasts |
|||
GF7 | Alphabioregen | 1ml frozen Vial | EUR 850 |
RFP-Expressing Human Neonatal Dermal Fibroblasts |
|||
RFP6 | Alphabioregen | 1ml frozen Vial | EUR 850 |
SV40-Human Intestine Fibroblasts |
|||
cAP-0206-SV40 | Angio Proteomie | 1Frozen Vial | EUR 5049 |
Rat liver fibroblasts |
|||
ABC-TC4153 | AcceGen | 1 vial | Ask for price |
Description: Rat liver fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Mouse liver fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium. |
Dog liver Fibroblasts |
|||
cAP-d0010 | Angio Proteomie | 1Frozen Vial | EUR 396 |
DHLF-As (Diseased Human Lung Fibroblasts - Asthma ) |
|||
LO194912 | Westburg | each | EUR 1673.23 |
DHLF-COPD (Diseased Human Lung Fibroblasts - COPD ) |
|||
LO195277 | Westburg | each | EUR 1673.23 |
hTert-Human Intestine Fibroblasts |
|||
cAP-0206-hTert | Angio Proteomie | 1Frozen Vial | EUR 5049 |
Mouse Lung Fibroblasts |
|||
ABC-TC3931 | AcceGen | 1 vial | Ask for price |
Description: Mouse lung fibroblasts, 2-week ICR mouse |
Rat Aortic Fibroblasts |
|||
ABC-TC4070 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Aortic Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Aortic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Aortic Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Artery Fibroblasts |
|||
ABC-TC4074 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Artery Fibroblasts from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Dermal Fibroblasts |
|||
ABC-TC4119 | AcceGen | 1 vial | Ask for price |
Description: Rat Dermal Fibroblasts (RDF) are derived from the dermis of normal Sprague Dawley rats. They are cryopreserved at first passage and can be cultured and propagated at least 8 population doublings. |
Rat Kidney Fibroblasts |
|||
ABC-TC4147 | AcceGen | 1 vial | Ask for price |
Description: Rat kidney fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Rat kidney fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium. |
Rat Spleen Fibroblasts |
|||
ABC-TC4222 | AcceGen | 1 vial | Ask for price |
Description: Rat Primary Spleen Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Spleen Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Spleen Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications. |
Rat Uterus Fibroblasts |
|||
ABC-TC4237 | AcceGen | 1 vial | Ask for price |
Description: Rat uterus fibroblasts, 6-week Wistar rat |